Subculture Study

Improved Essays
Preparing Subcultures. We obtained a stock vial with hot cheeto and a stock vial of wild type Drosophila melanogaster. We began our study of hot cheeto, a bristle mutation, by setting up subcultures of the mutant. We used the hot cheeto stock to create a new subculture to collect virgin females to be used for the genetic crosses. To reduce the chance of infection when creating subcultures, we disinfected the workplace station with a solution of 70% ethanol. Flies were anesthetized using the FlowBuddy device which regulates the carbon dioxide (CO2) gas for both, the gun used to anesthetize the flies inside the vial and the pad located underneath the stereomicroscope. We first anesthetized the flies within the vials. Once the flies were anesthetized, …show more content…
The complementation test cross was set up with the purpose of identifying the unknown gene. The cross was set up between parents with similar phenotypes. Two results were expected from this crosses, one of the crosses would show the complementation of two mutant alleles and the other cross would show two allelic mutations. If the two mutant allele complement each other all the progeny will be wild type, and thus are likely mutations in two different genes. However, if the progeny displays hot cheeto the two mutations are allelic and in the same gene. We crossed hot cheeto with singed. The progeny that appeared from this cross was all wild type. Indicating that they complement each thus hot cheeto cannot be singed. The other cross consisted of hot cheeto and forked. The progeny from this cross was all mutants therefore both mutations are allelic finally revealing that our unknown gene is …show more content…
The gene forked is located in the chromosome 1. It is a sex-linked recessive mutation. Even though our observational data provided us with the results necessary to identify forked, our statistical data deviated in all the genetic crosses except for the marker discriminant cross one control. For the marker discriminant cross one, our chi-square values showed a very large deviation when forked present. The chi-square value of the males was at 40.92 while females had a square value of 14.78. We think that the data might suggest that forked is having some genetic interaction with the marker genes thus deviating the expected data. Using more than three vials might have deviated our statistical results. The first genetic crosses revealed the inheritance pattern of the gen forked. This inheritance pattern allowed us to identify the gene. Further studies must be developed to understand more about the exact location of the gene in the chromosome, we also still need to research into the identity of the allele since our mutation does not look like f1. Future research should be based on the molecular basis of forked to understand better the nature of this

Related Documents

  • Decent Essays

    Meiosis Research Paper

    • 735 Words
    • 3 Pages

    This is what forms traits such as hair or eye color; genes from parents are mixed, and depending on the dominant/recessive alleles, the offspring could inherit one of the parents traits. Genetic diversity is caused by the crossing over of chromosomes during Prophase I, the random assignment of chromosomes at the equator during Metaphase II, and the random combination of gametes at fertilization. Say a red chromosome and a blue chromosome, homologous of course, are paired together during Prophase I, the chromosomes are crossed over, so now the blue chromosome contains a small amount of the red, and the red contains a small amount of the blue. This allows for genes to be mixed, which also leads to alleles being mixed from the two parent cells. Genetic diversity is caused by these variations that occur within meiosis simply because genetic material is mixed and passed on.…

    • 735 Words
    • 3 Pages
    Decent Essays
  • Decent Essays

    The intragenic recombination causes a distinct fragment pattern from both parents (Okagaki and Weil 1997). To easily discover the intragenic recombination, two individuals carried non-functional alleles of targeted genes, whose intragenic recombination causes a functional allele and obtained a distinct phenotype change, are priority used to develop a population for estimation of intragenic recombination rate. The functional alleles/plants in the progeny are resulted from intragenic recombination, and the ratio of functional allele plants to mutation allele plants was deemed as intragenic recombination rate.For example, the wxgene encodes a granule-bound starch synthase required for the synthesis of amylose in both the endosperm and pollen of maize and rice (Shure et al. 1983; Wang et al. 1990).Wxpollen grains stain deep blue in I2KI solution, whereas wxpollen grains stain light brown.…

    • 991 Words
    • 4 Pages
    Decent Essays
  • Improved Essays

    These different types of pairs rely on each other so that they can continue the process of making base pairs. If there is an omission, it will result in a mutation in the sequence and this will have to undergo the process of gene…

    • 1214 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    As a result, it makes up to ninety percent of human genetic variation and if SNPs happens in a gene sequence, it can cause a change in protein structure which can be detrimental. Moreover, a haplotype is referred as a combination of alleles or a set of SNPS that can be found in the same chromosome. 3. If PAV which codes for proline, alanine and valine is the original, therefore the AVI which codes for alanine, valine, and isoleucine is the missense codon (Overath, 2011). As a result, when there is an alteration in which a only one nucleotide changes to a different amino acid it’s a missense mutation.…

    • 1066 Words
    • 5 Pages
    Improved Essays
  • Superior Essays

    When DNA sequence appears in the genes, it has the same effect as mutations-nature can select for or against it resulting in evolution. Genetic drift, works on all mutations. It is the mechanism we relate to adaptation in an environment Darwin kept his ideas open, he knew that his ideas lacked efficient cause,” but hypotheses may often be of service to science, when they involve a certain portion of incompleteness, and even…

    • 1064 Words
    • 4 Pages
    Superior Essays
  • Improved Essays

    Crossing over is an event during meiosis when homologous chromosomes line up and exchange genetic information (Bhagat, R, et al.,2004). Resulting offspring follow set phenotypic ratio’s, which are presumed to be the null hypothesis that experiments are tested against (Brooker). In this experiment, the hypothesis for each of the crosses was that they follow the law of independent assortment resulting in a F2 generation with a 9:3:3:1…

    • 891 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    They Can occur in several ways likes when genes for formerly existing phenotypical features are conserved in DNA, and then these become expressed through a mutation that either knock out the overriding genes for the new traits or make the old traits override the new one. The Genes do not determine what the organism is, but with atavism, they can sometimes serve as reminders of our evolutionary past. However, there are disadvantages to atavism…

    • 930 Words
    • 4 Pages
    Improved Essays
  • Superior Essays

    Since Upf1 knockout is embryonic lethal but Upf1 heterozygotes are normal (Medghalchi, Frischmeyer et al. 2001), I will generate conditional Upf1 mutants. Previous studies have shown that majority of UPF1 mutations identified in pancreatic ASC promotes skipping of exons 10 and exon11 that cause a truncation in the essential RNA helicase domain. Mutations in the RNA helicase domain of UPF1 has been shown to be dominant negative(Sun, Perlick et al. 1998).…

    • 1047 Words
    • 4 Pages
    Superior Essays
  • Superior Essays

    Dna Synthesis

    • 1283 Words
    • 6 Pages

    The nick or spaces between the new DNA strands are joined using the enzyme DNA ligase resulting in the formation of two long daughter strands of DNA (Campbell et al., 2015). After the two daughter strands of DNA have been produced, it is time to proofread and repair the DNA sequence. This process is so important because any mistake in DNA replication can result to DNA mutation and will lead to various genetic diseases such as sickle cell anaemia, Huntington’s disease and many more (Cooper, 2000). Thus, the accuracy of DNA replication cannot be attributed solely to the specificity of the base pairing. According to Khan Academy (2017), to avert the mistake from happening, an enzyme which is DNA polymerase will proofread each nucleotide against its template as soon as it is covalently bonded to the growing strand.…

    • 1283 Words
    • 6 Pages
    Superior Essays
  • Improved Essays

    Besides that, I observed Long Branch lengths, frequent gene duplication and many duplicated genes subsequently become nonfunctional. These evidences suggest that the hypothesis of concerted evolution is not the best model fits to observed pattern of rbcS phylogeny in Angiosperms. Regarding the results, I hypothesize that the birth and death model (Nei and Hughes 1992, Ota and Nei 1994) of evolution can explain this pattern of rbcS evolution. In this model, new genes are created by repeated gene duplications, and some duplicate genes are maintained in the genome for a long time, but others are deleted or have become nonfunctional through deleterious mutations. I need further investigation to conclude that the observed pattern of rbcS…

    • 993 Words
    • 4 Pages
    Improved Essays

Related Topics