Subculture Study

2536 Words 11 Pages
Preparing Subcultures. We obtained a stock vial with hot cheeto and a stock vial of wild type Drosophila melanogaster. We began our study of hot cheeto, a bristle mutation, by setting up subcultures of the mutant. We used the hot cheeto stock to create a new subculture to collect virgin females to be used for the genetic crosses. To reduce the chance of infection when creating subcultures, we disinfected the workplace station with a solution of 70% ethanol. Flies were anesthetized using the FlowBuddy device which regulates the carbon dioxide (CO2) gas for both, the gun used to anesthetize the flies inside the vial and the pad located underneath the stereomicroscope. We first anesthetized the flies within the vials. Once the flies were anesthetized, …show more content…
The complementation test cross was set up with the purpose of identifying the unknown gene. The cross was set up between parents with similar phenotypes. Two results were expected from this crosses, one of the crosses would show the complementation of two mutant alleles and the other cross would show two allelic mutations. If the two mutant allele complement each other all the progeny will be wild type, and thus are likely mutations in two different genes. However, if the progeny displays hot cheeto the two mutations are allelic and in the same gene. We crossed hot cheeto with singed. The progeny that appeared from this cross was all wild type. Indicating that they complement each thus hot cheeto cannot be singed. The other cross consisted of hot cheeto and forked. The progeny from this cross was all mutants therefore both mutations are allelic finally revealing that our unknown gene is …show more content…
The gene forked is located in the chromosome 1. It is a sex-linked recessive mutation. Even though our observational data provided us with the results necessary to identify forked, our statistical data deviated in all the genetic crosses except for the marker discriminant cross one control. For the marker discriminant cross one, our chi-square values showed a very large deviation when forked present. The chi-square value of the males was at 40.92 while females had a square value of 14.78. We think that the data might suggest that forked is having some genetic interaction with the marker genes thus deviating the expected data. Using more than three vials might have deviated our statistical results. The first genetic crosses revealed the inheritance pattern of the gen forked. This inheritance pattern allowed us to identify the gene. Further studies must be developed to understand more about the exact location of the gene in the chromosome, we also still need to research into the identity of the allele since our mutation does not look like f1. Future research should be based on the molecular basis of forked to understand better the nature of this

Related Documents

Related Topics