Using Appendix E of the General Microbiology Laboratory lab handbook, I narrowed down the unknown microorganism to Enterobacter aerogenes. Every test matched with my experimental results except for the fermentation of lactose. According to Appendix E, E.aerogenes is able to ferment lactose however my enterotube experiment yielded a negative result. I assume this difference is due to human error of misidentification of the color of the enterotube chamber. Besides, the MacConkey plate yielded a…
should avoid whey protein as a part of their diet. If a person has lactose intolerance, it is not wise that these individuals use whey. Also, different whey protein powders come with a varying amount of calories, fat, and lactose content. Whey protein Isolates are about 90% protein and very little to no fat, cholesterol, or lactose. Whey concentrates can have 29%-90% protein, and as proportion of protein goes down, the fat and lactose levels come up. Protein supplements of any kind may cause…
and also a cure to people, but what I am saying is that don’t look at the bad side, but the bright side is really what counts when it comes to being healthy for the body. The main purpose of milk is to help a person grow, but some people are very lactose, which prevents a lot of people from using milk, which is a big reason people don’t buy too much milk nowadays. Sales prices are going down due to the people who don’t want milk because the side effects of being on and off with it; For…
this experiment an unknown sample was obtained randomly to identify the possible microorganisms. Using comparative analysis, different test were performed to rule out which organism was given. The tests performed were an Oxidase test, PR Glucose, PR Lactose, Pr Sucrose, Indole test, and a Urea Agar test. In order to determine the organism characteristics the sample was isolated in a streak plate and incubated at both 25 and 37 degree celsius. Once growth was found the colonies were gram stained…
inhibits the growth of most Gram-positive organisms and can determine if an isolate is a lactose fermenter. If the isolate were a lactose fermenter this would be indicated by a dark color change in the colonies that formed. The EMB test results showed isolate 6B to form a metallic dark blue color, which, indicated isolate 6B was most likely a Gram-negative organism and a lactose fermenter. To ensure isolate 6B was a lactose fermenter, a test on MacConkey agar was performed. MacConkey agar is…
These solutions include glucose, maltose, honey, lactose and beer. There was precipitation formed in these solutions, which resulted in the colour change indicating that sugar must be present in these solutions for this to occur (Nigam et.al, 2003). Monosaccharides such as glucose, maltose and lactose are all defined as simple sugars and since they showed a positive result in this test, it indicates that the Benedict’s test…
Identification of Unknown Bacterium #8 Report. Aim: The objective of this report is to identify the unknown bacteria by completing different tests. The test includes things such as a Nutrient agar plate, MacConkey’s agar plate, Gram staining, Oxidase test, UTI ID Membrane, and Eosin Ethylene Blue Agar. Materials: Testing Unknown Bacteria #8 Materials: 1. Nutrient Agar Plate (NA) 9. Hydrogen Peroxide - 2 g/ml. MacConkey’s Agar Plate (MAC) 10. UTI filter 3. Eosin Methylene Blue Agar Plate (EMB)…
Carbohydrate Utilization: Materials: - Phenol red lactose Broth - Phenol Red Sucrose Broth - Unknown organism # 40 - Inoculating tool Procedure: Phenol red lactose and Phenol Sucrose were cultures used to identify fermentation of carbohydrates for the unknown organism #40. Phenol red is a ph. indicator as the inverted Durham tube indicates gas production. The unknown organism was inoculated into each phenol red broth with either sucrose or lactose. The inoculating tool was sterilized by heat…
Lactose Intolerance: Lactose bigotry is a standout amongst the most well-known whey protein reactions. I know numerous individuals that experience this issue when they take whey protein shakes. On the off chance that you are one of those, your most solid option is to run with whey protein isolate in light of the fact that it contains just 1% of lactose, which is low contrasted with the common 5-6% or standard whey protein. Generally individuals with lactose narrow mindedness switch…
Love in the Sahel In the Fulani culture the men are known as the cattle herders. It has been a tradition for centuries that every year the men leave home, leaving behind their girlfriends, wives and families to go on a eight month migration with their cattle. They do this for one specific reasons, and that is to feed their cattle. While on this migration they have one goal to accomplish and that is to return home with fat and healthy cattle. In the Fulani culture, the women’s parents choose who…