bit of background information to know about the Brassica lab, in order to fully understand what is being tested and the mechanisms behind it. There are questions that need to be asked such as: why use Wisconsin fast plants, what gamma radiation does to organisms on a molecular level, and even what it does to organisms on a physical level. Why did we use Wisconsin fast plants for this experiment? Wisconsin fast plants are more formally known as Brassica Rapa and are rapid cycling plants. They…
Similarity indices and genetic distance The overall mean similarity index for Brassica oleracea accessions calculated based on all AFLP fragments amplified using Nei’s (1978) similarity index, ranged from 0.297 to 0.999 with an average of 0.744 (Table 3). The highest similarity indices (0.999) and the lowest genetic distance (0.001) were between the accessions of the same crop variety and geographical region, e.g. spring cabbage HRIGRU4564 and HRIGRU4571 from Cork. Accessions having close…
The rapid-cycling Brassica rapa was developed by Dr. Paul Williams over a fifteen year period of using simple breeding techniques. These Brassica rapa plants develop remarkably quickly, flowering between thirteen and eighteen days after being planted. These plants are relatively small but need a continuous amount of water and cool white fluorescent lighting. Students in the botany lab were told to conduct experiments and to conduct research using the Brassica rapa. The purpose of the experiment…
steady growth rate in stem elongation in the rosette Brassica rapa that were treated with the gibberellic acid. This is because the rosette plants had a gene mutation that caused a deficiency of GA in the plants’ genetic structure as mentioned in the introduction, which without the combination of additional gibberellic acid and the necessary water to activate it would result in a lack of growth. This mutation also caused the rosette-type Brassica rapa, that was only given water, to grow at a…
characterise Brassica oleracea accessions. Among the 136 reproducible fragments generated, 25 (18.4%) fragments were common for all Brassica, 27 (19.9%) were unique and 84 (61.7%) were phylogenetically informative. Flannery et al. (2006) assessed polymorphisms in Brassica, Arabidopsis, Camelina, Raphanus and Sinapis using 10 plastid SSR primer sets. Eight loci were polymorphic, and separated the individuals of Brassicaceae into taxon-specific groups (Arabidopsis, Camelina, Sinapis and Brassica…
Determination of GAPC Gene Sequence for Brassica Oleracea var. acephala and Brassica Oleracea var. capitate INTRODUCTION GAPDH is an enzyme that is encoded by the GAPC gene and catalyzes glycolysis’ sixth step which is responsible for energy metabolism (Bio Rad 2017). Aside from its role in glycolysis, GAPDH is also known for its evolutionary significance specifically that it has roles in cellular function like nuclear RNA export and DNA replication and repair (Tatton et al. 2001). GAPDH has…
Introduction: Brassica Rapa comes from the family of Brassicaceae, also known as the mustard family; the genus Brassica L is known as mustard, and the species Brassica Rapa L is commonly known as field mustard (Plant Profile). There are several subspecies of Brassica Rapa, like a turnip, and they all have varying uses like oil and root production. We are using the Wisconsin Fast Plants that have artificially selected to be optimal for educational use. We are trying to increase the number of…
These Wisconsin Fast plants are a member of the family Brassica, called Brassica Rapa. The Brassica, family consist of many commercially produced consumable goods, such as, greens, broccoli, cauliflower, Brussel sprouts and turnips. The Brassica Rapa is native species commonly found throughout the continents of Europe and Asia. The seeds of the Brassica Rapa, were known for their disease resistance that commonly affected crops in the United States. In…
Genetics and fine mapping of a purple leaf gene, BoPr, in ornamental kale (Brassica oleracea L. var. acephala The Ornamental Kale, also known as the “brassica oleracea L. var. acephla”, is a popular widely known plant worldwide due to the beautiful diverse colors that leaves display. Its name, which derived from the Latin language means a cabbage leaf. The colors consist of: a hunter green, greenish-gray, or yellowish-white with its center consisting of white, purple, a rosy pink and many other…
When you think of bone broth, do you think of it as meat water? Well, this meat water has plenty of benefits! But what makes it so special? The answer is easy – collagen! Not only does bone broth provide you with a healthy gut, it also gives you radiant skin and a boosted immune system! Keep reading below to find out what bone broth can do for your body! Heal Your Gut Did you know that having a cup of bone broth a day works miracles for those who suffer from leaky gut syndrome? It is also good…