Determination of GAPC Gene Sequence for Brassica Oleracea var. acephala and Brassica Oleracea var. capitate
INTRODUCTION
GAPDH is an enzyme that is encoded by the GAPC gene and catalyzes glycolysis’ sixth step which is responsible for energy metabolism (Bio Rad 2017). Aside from its role in glycolysis, GAPDH is also known for its evolutionary significance specifically that it has roles in cellular function like nuclear RNA export and DNA replication and repair (Tatton et al. 2001). GAPDH has been found in apoptosis, neurodegenerative disease, and viral pathogens which is part of its biomedical significance (Tatton et al. 2001). In addition, Altenberg and Greulich (2004) claim that genes like GAPC that are involved in glycolysis have been …show more content…
The gDNA for the control, A. thaliana was supplied. PCR was then utilized in two rounds with the second using nested PCR. Next, the PCR results were analyzed, and the PCR was purified. Following this step, the PCR product was ligated into a plasmid vector and bacteria was transformed. Next the plasmid had to be isolated from the bacteria and this allowed for sequencing of the DNA (Bio Rad 2017).
The ultimate goal of this study was to determine the gene sequence of both Brassica Oleracea, var capitate and Brassica Oleracea var. acephala while verifying the gene sequence of Arabidopsis thaliana.
REFERENCES
Altenberg B, Greulich KO. 2004. Genes of glycolysis are ubiquitously overexpressed in 24 cancer classes. Genomics. 84(6):1014.
Bio Rad. 2017. Plant Technology. 1st ed.
Gardiner J, Marc J. 2011. Arabidopsis thaliana, a plant model organism for the neuronal microtubule cytoskeleton. Experimental Botany. 62(1):89
Maggioni L. 2015. Domestication of Brassica oleracea L. Acta Universitatis. 74(1): 5-13.
Tatton WG, Chalmers-Redman RME, Elstner M, Leesch W, Jagodzinski FB, Stupak DP, Sugrue MM, Tatton NA. 2001. Glyceraldehyde-3-phosphate dehydrogenase in neurodegeneration and apoptosis signaling. Advances in Research on Neurodegeneration.
INTRODUCTION
GAPDH is an enzyme that is encoded by the GAPC gene and catalyzes glycolysis’ sixth step which is responsible for energy metabolism (Bio Rad 2017). Aside from its role in glycolysis, GAPDH is also known for its evolutionary significance specifically that it has roles in cellular function like nuclear RNA export and DNA replication and repair (Tatton et al. 2001). GAPDH has been found in apoptosis, neurodegenerative disease, and viral pathogens which is part of its biomedical significance (Tatton et al. 2001). In addition, Altenberg and Greulich (2004) claim that genes like GAPC that are involved in glycolysis have been …show more content…
The gDNA for the control, A. thaliana was supplied. PCR was then utilized in two rounds with the second using nested PCR. Next, the PCR results were analyzed, and the PCR was purified. Following this step, the PCR product was ligated into a plasmid vector and bacteria was transformed. Next the plasmid had to be isolated from the bacteria and this allowed for sequencing of the DNA (Bio Rad 2017).
The ultimate goal of this study was to determine the gene sequence of both Brassica Oleracea, var capitate and Brassica Oleracea var. acephala while verifying the gene sequence of Arabidopsis thaliana.
REFERENCES
Altenberg B, Greulich KO. 2004. Genes of glycolysis are ubiquitously overexpressed in 24 cancer classes. Genomics. 84(6):1014.
Bio Rad. 2017. Plant Technology. 1st ed.
Gardiner J, Marc J. 2011. Arabidopsis thaliana, a plant model organism for the neuronal microtubule cytoskeleton. Experimental Botany. 62(1):89
Maggioni L. 2015. Domestication of Brassica oleracea L. Acta Universitatis. 74(1): 5-13.
Tatton WG, Chalmers-Redman RME, Elstner M, Leesch W, Jagodzinski FB, Stupak DP, Sugrue MM, Tatton NA. 2001. Glyceraldehyde-3-phosphate dehydrogenase in neurodegeneration and apoptosis signaling. Advances in Research on Neurodegeneration.