Bacterial growth

However, the indole test from the SIM test, citrate test, and the growth of the EMB plate was deemed to be the most important test because the unknown bacteria was tested positive for indole, negative for citrate, and had metallic green sheen, with purple and black centers on it. This indicates that unknown 18 for the…

In addition, procedure part H contains the viewing of cheek cells, cheek cells are a high-caliber option for identifying the membrane, and cytoplasm of a cell. To begin, place a drop of methylene blue on a slide, then smear a toothpick amongst the inside of the cheek, lastly smear the toothpick onto the stained slide. Cheek cells just like starch cells are hard to be viewed without the addition of a staining chemical, in this case, the methylene blue will make the cheek cells have a slight blue…

bacteria can be a difficult process due to the fact that individual bacterial cells can possess similar structural morphology to other types of bacteria. As we have demonstrated in previous lab experiments there are key biological and chemical processes that are characteristic to different types of bacteria. Using various biochemical tests we can evaluate and detect these different processes to aid in our identification of unknown bacterial samples. The implications of these test results can be…

circumstance the bacteria could be resistant to that particular drug. (5 pts) As the name implies 50s subunit protein synthesis inhibitors such as erythromycin inhibit protein synthesis by binding to the 50S subunit of the 23S rRNA molecule of the bacterial ribosome. This ultimately acts to block the exit of the growing peptide chain. It should be noted that gram+ bacteria more effectively accumulate erythromycin than gram negative bacteria thereby making it more effective in this group of…

It is a selective media because, it kills Gram positives but allows Gram negatives to survive. If there is growth present on the EMB media it means it is a Gram negative rod. The color of the results determine if lactose fermentation is present. If the sample is colorless then there is no lactose fermentation present. If the bacteria is purple then lactose fermentation…

After part 1, the experimenters found two things about the Bacteria: First, that the bacterium was bacilli (rod) shaped. This was determined by viewing individual cells through a microscope at 400x power. Secondly, that the bacterium was most likely a Gram-stain-negative because it appeared to be a pink color after gram-staining. This had several implications: The experimenters now knew the bacteria’s cell wall composition, its outer membrane composition, peptidoglycan concentration, outer…

adjusted bacterial concentration inoculum (5 × 105 CFU/mL) were added in the 96-well micro-titer plates, the growth control wells contained MH broth medium with tested bacterial concentrations and sterility control wells contained only MH broth medium as shown in figure 2. The plates were then covered to ensure that the bacteria were not dehydrated. Then the plates were incubated at 37°C for 18 to 20 hours. The lowest concentration of each antibacterial agent that inhibited the bacterial growth…

fast. This is proved by the fact that biotechnology has a growth rate of 7 percent in the market of food and pharmaceutical applications. The advent of the industrial revolution resulted in impairment of the environment through the release…

experiment was to examine the different growths of minority organisms within a variety of media cultures. These cultures included types of selective, differential, and enriched media and were applied to split sections on the agar plate. Each of the four broth cultures: Escherichia coli, Enterobacter aerogenes, Staphylococcus epidermidis, and Streptococcus faecalis showed observable growth when applied. The different medias will either promote or inhibit the growth of the organisms allowing their…

The spectrophotometer analysis of Escherichia coli growth during heat application The purpose of this experiment is to determine the rate of Escherichia coli (E. coli) growth over 100 minutes in a liquid growth medium (control), and to determine the rate of E. coli growth over 100 minutes in a heated liquid growth medium. The prediction is that the control E. coli culture will grow at a faster rate exponentially over 100 minutes than the experimental bacteria culture. Consequently due to heat…