Bacterial growth

We first needed to collect all the proper materials to conduct the tests on our unknown bacterial sample. To begin the lab, we had to collect a nonpathogenic bacterial sample by swabbing an item or surface inside the biology building with a sterile cotton swab. Our lab group decided to swab a Sharpie permanent marker left at our lab station inside our lab room. We then opened a petri dish and smeared the swab across the surface of the agar several times. Once the bacteria was on the agar, we…

Materials and Methods Overview The whole study consists of four phases. To start with, the suitable media need to be identified for the growth of Av. paragallinarum. Secondly, the media used in disc diffusion and broth dilution testing need to be validated. This validation process is completed by performing disc diffusion and broth dilution testing of quality control strain on candidate agar or broth. Then, the antimicrobial sensitivity of Av. paragallinarum can be tested on validated media, by…

two unknown bacterial colonies. The plates were then incubated for 12 hours at 37 ̊C. The blood agar plate was used to create a greater contrast in color to help distinguish between bacterial colonies by size and color. Two distinct colonies could be seen growing on the blood agar plate shown in figure 1. The smaller, opaque colored bacterial colonies were labeled “A” and the larger, more transparent bacterial colonies were labeled “B”. After observing the different colony growth, isolation…

and ampicillin [LB/amp], another plate contained LB broth, ampicillin, and arabinose [LB/amp/ara], and the last plate contained only LB broth [LB]. We pipetted 100 ul of transformed bacterial suspension to the center of an LB/amp plate and then to the LB/amp/ara plate. Next, we pipetted 100 ul of untransformed bacterial suspension to the center of an LB/amp plate and the LB plate; these plates served as our control plates. We used a new sterile loop for each plate to spread the…

specimens. Journal Entries: April 17, 2018. Today we began the unknown project. I obtained the unknown broth tube #15. I used two Tryptic Soy Agar (TSA) plates in order to make a streak plate. The streak plate is used to separate the two different bacterial organisms that were present in the broth. The plates were placed in the incubator at 37C and in 48 hours there should be isolated colonies on the plates. April 19, 2018. I obtained my TSA plate from the incubator and determined that…

37 °C for 48 hours. This process was done on October 11th and repeated on October 18th. The growth was observed and recorded in Table 4. A sterile inoculating loop was used to quadrant streak samples of Staphylococcus epidermidis and Micrococcus luteus on agar plates. This process was done on October 11th and repeated on October 18th. The plates were incubated at 37 °C for 48 hours. The resulting growth was recorded in Table 6. A sterile inoculating loop was used to spot inoculate Escherichia…

Mtb is classified as a Gram-positive bacteria, and is one of the few bacteria that can be cultured in the laboratory5. Its cell surface is partly composed of various virulence lipids, such as phthiocerol dimycocerosates (PDIMs) and phenolic glycolipids (PGLs). and mycolic acid3. Mtb, like many other bacteria, is capable of sensing and responding to their surrounding environment. It does so via the use of two-component systems, one of which is called PhoPR two-component system6. In order for Mtb…

Drosophila hemolymph, IPCs are necessary for carbohydrate homeostasis. When IPCs are explicitly removed, developmental delays occur growth is hindered. Late-life mortality is diminished in these model organisms, suggesting that life span is regulated by dilps secreted from these pancreatic β cells. c. In specific target tissues, dilps activate the insulin-like growth factor receptor (InR). InR signals through chico, a homolog of the mammalian insulin receptor substrates, PI3 kinase (PI3K), as…

was to learn the proper procedures and techniques used on daily basis by microbiologists and other working professionals. Some of the same procedures used throughout this lab are used by scientists working to discover more information on their own bacterial species (1). Additionally, one learned how to organize and keep up to date a laboratory notebook that contains pertinent information. By using the lab notebook with systematized exercises’ results, one categorized the environmental isolate’s…

an error while contaminating the second Petri plate as no bacteria colony was observed on the surface of agar plate. The stool and sink in the laboratory was probably disinfected with 70% alcohol and anti-bacterial agent before the experiment was done resulting in the lack of microorganism growth on the agar surface. The bacteria might also be obligate anaerobes that cannot live in the presence of…