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18 Cards in this Set
- Front
- Back
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What is the application for the TSI Agar?
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Used to differentiate members of Enterobacteria and to distinguish them form other Gram-negative bacilli.
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What is in the media of the TSI?
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Glucose. Lactose. Sucrose. Beef extract. Yeast extract. Peptone. Sodium thiosulfate. Phenol red. Ferrous sulfate.
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What is the purpose of the triple sugars in the medium?
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Used to differentiate bacteria on the basis on specific carbohydrate fermentation.
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What is the the purpose of beef extract and yeast extract and peptone in the media?
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Used as sources of carbon and nitrogen.
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What is the purpose for sodium thiosulfate in the media?
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Used as a source of reducible sulfur.
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What is the purpose for phenol red in the media?
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pH indicator.
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What is the purpose of ferrous sulfate in the media?
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Used as the hydrogen sulfide indicator.
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If the butt of an inoculated tube is totally black
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what color do you assume the slant is?
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Hydrogen may be produced by the reduction of ___________ in the medium or by ________________.
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Thiosulfate or by the breakdown of cysteine in the peptone.
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If the medium has turned red (slant and butt) what has happened?
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No fermentation Peptone catabolized aerobically and anaerobically with alkaline products. Not Enterobacteria.
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TSI is used to primarily differentiate members of what family?
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Enterobacteriaceae.
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What is the interpretation for a result of yellow slant/yellow butt?
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Glucose and lactose and/or sucrose fermentation with acid accumulation in slant and butt.
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What is the interpretation for a result of red slant/yellow butt?
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Glucose fermentation with acid production. Peptone catabolized aerobically in the slant with alkaline products (reversion)
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What is the interpretation for a result of red slant/red butt?
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No fermentation. Peptone catabolized aerobically and annaerobically with alkaline products. Not from enterobacteria.
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What is the interpretation for a result of red slant/no change in the butt?
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No fermentation. Peptone catabolized aerobically with alkaline products. Not Enterobacteria.
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What is the interpretation for a result of no change in slant/no change in butt?
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Organism is growing slowly or not at all. Not from Enterobacteria.
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What is the interpretation for a result of Black precipitate in the agar?
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Sulfur reduction. (An acid condition (from fermentation of one or more of the sugar) exists in the butt even if the yellow color is obscured by the black precipitate).
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What is the interpretation for a result of cracks in or lifting of agar?
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Gas production.
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