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19 Cards in this Set

  • Front
  • Back

Investigate how light intensity effects the abundance of plant species in an area

• IV - dif light intensity


• DV - % cover to estimate the % of area of the quadrat taken up by each species


• CV - 1) mineral contents of soil as this effects the growth of plants 2) water content of soil as used in photosynthesis so effects growth


• repeats > reliable & statistical tests to see if real dif


• belt transect & systematic sampling > each 2nd quadrat used

Transect

A line across a habitat

Line transect

Records species making contact with the tape measure at regular intervals

Belt transect

Uses quadrats places at regular intervals along the transect to measure the abundance of species


Using 1) % cover & 2) freq

Random sample

- uniform area


- randomly select area using a random no. generator & co-ordinate grid

Systematic sampling

- non uniform area


- measurements taken at regular & fixed intervals

Percentage cover

An estimate of how much of the quadrat is covered by a particular species

Creation of ATP in photosynthesis

• ADP + inorganic phosphate > ATP (phosphorylation)


• CONDENSATION reaction


• chemical energy is stored in the phosphate bond


• requires energy > from moving protons down ATP synthase

Creation of ADP in photosynthesis

• ATP +H20 > ADP + inorganic phosphate (+ energy)


• HYDROLYSIS > uses water (hydro) to split (lysis) ATP


• releases energy (chemical energy released from the phosphate bond)


• used in biological processes e.g. contraction of cardiac muscle

DCPIP

• blue > colourless when reduced (accepting electrons)


• takes place of NADP


• colour change indicates that photosynthesis has occurred

Investigate photosynthesis using isolated chloroplasts

• IV - dif light intensities


• DV - rate of photosynthesis measured by the amount of DCPIP reduced (rate of colour change from blue to colourless)


• CV - DCPIP of the same volume (takes longer to decoulrise more), temperature (water baths), chloroplasts from same species


• amount of time recording colour change


• repeats > reliable, means & statistical tests


• use of colorimeter

Use of colorimeter

• set up colorimeter with a red filter (because DCPIP is a blue liquid)


• zero it using a clean cuvette containing (chloroplast extract &) distilled water


• then used to measure absorbance of light

Investigate the effect of temperature on the initial rate of an enzyme catalysed reaction

• IV - temperatures e.g. use water baths at 10, 20, 30, 40, 50 degrees


• DV - the amount of gas produced (oxygen) is measured


• record gas every 10 seconds for 3 minutes using stopwatch


• CV - conc. & volume of substrates, pH of solution


• repeats > means, reliable, stats tests


• equipment; gas syringe, conical flask, bung, (catalase & hydrogen peroxide)

What is Q10 & how is it calculated?

The factor by which the rate changes with every 10 degrees rise in temperature


Q10 = rate at T+10/ rate at T degrees


Does not apply above the optimum

Investigate the effects of temperature on seedling growth rate

• IV - temperature at water baths of 10, 20, 30, 40, 50 degrees


• DV - height measured as an indication of growth


• CV - same species plant (get clones that are genetically identical), minerals in soil, water content of soil/ light exposure (effects photosynthesis)


• repeats > plant many e.g. 5 for each condition in the same pot


• allow to grow for same time period e.g. 2 weeks

Investigate the effects of temperature on the the hatching rate of brine shrimp

• IV - temperature at water baths of 10, 20, 30, 40, 50 degrees


• DV - count no. of brine shrimp after 24 hrs


• CV - starting no. of brine shrimp eggs, volume of water, time interval left for at each temperature


• repeats

Carbon cycle applied to reduce Co2 levels

1) reforestation > plants photosynthesise so reduce Co2


2) decrease burning of fossil fuels > less Co2 released

Main aspects of carbon cycle

1) Co2 absorbed > photosynthesis


2) Co2 released > respiration, combustion, decomposition


3) carbon sinks (in areas w/ no decomposers)

Cyclic photophosphorylation

• only uses PSI & does not reduce NADP


• light absorbed by chlorophyll in PSI


• excited electrons enter ETC > move H+ > generation of ATP


• cyclic as the de energised electron returns to PSI