Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
11 Cards in this Set
- Front
- Back
|
Name and describe four different mechanisms by which protein activities are regulated |
1.) Gene expression: 2.) Compartmentalization: 3.) Altering protein conformation: Binding to allosteric sites, covalently attaching bulky/charged group. (Example: phosphorylation 4.) Proteolytic cleavage |
|
|
What is the difference between a competitive and an noncompetitive antagonist? |
Competitive antagonists bind at the same site as receptor agonists. Non-competitive antagonists bind at an allosteric site that changes the conformation of the receptor, preventing the agonist from binding. |
|
|
How does a competitive antagonist affect Km and Vmax? |
Competitive enzyme inhibitor will increase the Km, which will increase the slope and x-intercept. However, the y-intercept will remain unchanged because Vmax remains the same. |
|
|
How does a non-competitive antagonist affect Km and Vmax? |
Noncompetitive enzyme inhibitor will decrease Vmax so it will increase the slope and y-intercept. However, the x-intercept will remain unchanged because Km does not change. |
|
|
What is the slope in a Lineweaver-Burke plot? |
Km/Vmax |
|
|
What is the x-intercept for a Lineweaver-Burke plot? |
-1/Km |
|
|
What is the y-intercept for a Lineweaver-Burke plot? |
1/Vmax |
|
|
Describe the path that proteins take from nucleus to exocytosis |
mRNA is produced in the nucleus mRNA travels to RER, where translation occurs Folded proteins travel to Golgi Apparatus to be sorted for secretion/transport to lysosomes, plasma membrane, or secretory vesicles |
|
|
Why do plasma cells contain so much RER? |
RER is needed to synthesize antibodies |
|
|
What are the three classes of structure in the cytoskeleton? |
microfilaments, intermediate filaments, and microtubules |
|
|
Why does the nucleolus stain dark blue in an H&E stain? |
Nucleolus is responsible for synthesizing rRNA. Nucleic acids are acidic and thus basophilic, attracting hematoxylin (blue) |
