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16 Cards in this Set
- Front
- Back
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Dioxane is a reagent that can be used
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for both dehydrating and clearing tissues
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The chief objection to the use of xylene as a clearing agent for processing tissues is that xylene
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likely to harden tissue
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Overheating of the paraffin used to embed may cause
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cracking of the block
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Paraffin is cooled down as fast as possible to prevent
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formation of large ice crystals
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the dehydration and clearing steps can be omitted when using
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water soluble wax
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Should bone sections be embedded parallel to the long axis of the block
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NO it should be diagonal
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Formic and Nitric acid are both used for
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Decalcifying
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Nitric acid impairs
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staining reactions
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After how many hours will nitric acid damage tissue
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48
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Is formic or Nitric acid used with ion exchange resins
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Formic
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Tetrahydrofuran is what type of solvent
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universal
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Will xylene and tetrahydrofuran clear tissue for infiltration
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yes
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chloroform and cedar wood oil are both
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clearing agents
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Does chloroform make tissue transparent
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NO
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When undecalcified bone is to be embedded it must be embedded in
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plastics/resins
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What is used to check end point decalcification
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ammonium oxalate which precips the phosphates.
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