Processing

Front 1

1.What is the problem shown

2. how do you prevent this problem

3. which layer is missing from the section

Back 1

1. The section of small intestine has rolled and is not oriented correctly

2. Gastrointestinal specimen should be opened and pinned

3. Muscularis externa cannot be seen

Hint 1

Small intestine has 4 layers that should be seen

Front 2

1. What is the tissue shown

2. What is the problem and how could it be prevented

3. The problem show is due to

Back 2

1. Bone marrow

2. Carefully monitoring the endpoint of decalcification

3. Overdecalcification

Front 3

What is this specimen

Back 3

Bone marrow

Hint 3

Notice red blood cells

And (white) cavities

Front 4

1. What is the problem shown

2. How could it be prevented

Back 4

1. Improper embedding , tissue was not flat like in the block

2. Ensuring that both sections are embedded flat and at the same level

Front 5

1. What is the problem shown

2. How do you prevent this

3. The problem occurred during which step

Back 5

1. Forcep metastasis , the tissue in the upper right is from another specimen

2. Prevent by better cleaning of forceps between specimen

3. Occurred during embedding

Front 6

What is the problem and how to prevent

Back 6

Parched earth

Cause: over dehydration

Fix: soak in ice water before cut

Front 7

What is the Problem and how to fix

Back 7

Dry/ brittle powdery tissue

Cause: over dehydration

Fix: soak in water before cut

Front 8

What is the problem and how do you fix it

Back 8

Nuclear bubbling

Cause: incomplete fixation

Fix: reprocess

Front 9

What is the problem and how do you fix it

Back 9

Crosshatching Sponge artifact

Cause : Placing the tissue between 2 dry sponges

Front 10

What is the problem

Back 10

Washed out epithelial nuclei

Cause: contaminated(water) clearing/infiltration

Front 11

What is the problem

Back 11

Micro chatter

Cause: over dehydration

Front 12

1. What is the problem shown, how to prevent it

2. Best corrected by removing cover glass and ..

3. The problem demonstrated is

Back 12

1. Dehydrating the section completely

2. Re-dehyrating and reclearing with fresh solution

3. Incomplete dehydration of section

Hint 12

There are water droplets

Front 13

1. What is the problem and what is one possible cause

2. How to correct this

Back 13

1. Incomplete deparaffinization

Causes:

- Incomplete removal of paraffin

- water left from incomplete drying

2. Treat w/ xylene and alcohol and decolorize and restain

Hint 13

Notice White spots

Front 14

1. What is the problem and what is it caused by

2. How to correct in the future by decreasing ..

3. This problem would not occur if aluminum hematoxylin type had been

4. What is the tissue

5. What is the epithelium shown

Back 14

1. Inadequate differentiation

2. Hematoxylin

3. Mayer (difficult to overstrain)

4. Skin

5. Keratinizing stratified squamous

Hint 14

Notice top is too Dark and nuclei is not reddish-purple or acidophilic

Front 15

1. The cells showing intense red cytoplasm are

2. The stain shown is

3. The tissue shown is

Back 15

1. Erythrocytes

2. Giemsa

3. Bone marrow

Hint 15

blood cells resemble little dots

What stain is used for hematopoietic tissue

Front 16

1. What is the problem

2. How can it be prevented

3.what is the tissue structure in the upper left part of the

Back 16

1. Eosin not showing 3 shades (not properly differentiated )

2. Differentiating the eosin better in the lower dehydrating alcohol

3. Blood vessel

Front 17

1. What is the problem

2. What could cause this

3. The problem can be corrected by using a mounting medium that is …

Back 17

1. Evaporation of the mounting medium

2. Causes:

- warped cover glass

- mounting medium too thin

3. Corrected by using fresh mounting medium

Front 18

1. The cells that show intense pink cytoplasmic staining are most likely

2. the stain shown is

3. The intense rose color of the cytoplasm is due to

Back 18

1. Plasma cells

2. Methyl green - pyronin

3. Rough endoplasmic reticulum

Hint 18

Stain shows

red - RNA ( ribosome)

Blue - DNA ( nuclei)