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152 Cards in this Set

  • Front
  • Back

Solute potential of soil


(numbers)

-0.02 MPa (negligible)


-0.2 MPa in saline soil

Pressure potential for Soil


(numbers)

Wet soil- close to 0


As soil drys, becomes more negative

How does water move through soil

Bulk flow in response to pressure gradient

How does the water move?
How does the water move? (what type of potential)

Soil- pressure potential


Across root- water potential


In xylem- pressure potential


Leaf air spaces- water vapor concentration

Root Hairs


(definition)

Filamentous outgrowths of root epidermal cells that increase the surface area of the root

Explain the picture

Explain the picture

Mature regions of the root are less permeable. This allows most of the water uptake to occur in new growth in new areas!

Pathways from epidermis to endodermis

Apoplast


Symplast


Transmemebrane

Apoplast


(definition, function)

water moves through cell walls and extracellular spaces w/o crossing any membranes as it travels to the root cortex

Symplast


(definition, function)

Water travels across root cortex via plasmodesmata in the cell

Transmembrane pathway


(definition, function)

water enters a cell on one side and exits the cell on other


Crosses plasma membrane twice in each cell. May also cross tonoplast

Tonoplast


(definition)

Membrane that surrounds vacuole



Caspian strip


(definition, function)

Hydrophobic strip between cell walls of endodermis. Forces water to go through endodermis membranes and aquaporins

Root pressure


(definition)

Positive hydrostatic pressure in the xylem of roots.


Caused by build up of solutes and low transpiration eg- night time and dew drops

Guttation


(definition)

Exudation of liquid from leaves due to positive root pressure

Xylem Parts


(types, function)

Tracheids- elongated hollow dead cells with highly lignified walls


Vessel Elements- Dead cells, shorter and wider than tracheas, have perforation plates at each end of the cell. Stack and form a longer tube called a vessel

Conifer Xylem pit

Conifer Xylem pit

A- Margo


B- Torus


C- Pit Cavity

Other Vascular Plants

Other Vascular Plants

A- Secondary cell walls


B- Pit Membrane


C- Pit Cavity


D- Primary cell walls

Pressure-driven Bulk Flow


(function in plant)

Responsible for long-distance transport of water in xylem


Independent of solute concentration gradients


Xylem provides low resistance pathway for water

Cohesion-tension theory of sap ascent


(explain ESSAY QUESTION)

As water evaporates, it pulls more water up to the top of the plant. Requires cohesive properties of water to sustain high tension in the xylem water columns

How much pressure to move water to the top of a tree?


(number)

2MPa

Xylem cavitation and how the plant minimizes the problem

-The loss of tension in the xylem due to the formation of a gas bubble


-positive pressure can sometimes break bubble


-Water flows around bubble and new xylem forms

Major factors for transpiration

1- difference in water vapor concentration between the leaf air spaces and external bulk air


2-Diffusional resistance (r) of this pathway

Diffusional Resistance (r)

Two components- leaf stomatal resistance and boundary layer resistance

Leaf stomatal resistance

associated with diffusion through stomatal pore


resistance of CO2 diffusion through stomatal pore

Leaf boundary layer resistance

resistance to diffusion of water vapor due to layer of unstirred air next to leaf surface

What is the driving force for transpiration?

-Difference in water vapor concentration!


-Must be lower outside of cell than inside cell so water moves out

Stomata- function

-Leaf transpiration


-Changes in stomatal resistance important for water loss regulation


-Temporal regulation


-When water is not abundant, stomata will close to retain water



Abscisic Acid


(Definition, function)

Hormone that helps control opening and closing of stomata pores


Works with specialized epidermal cells and guard cells

Stomatal Complex

(parts)



Guard cells, subsidiary cells, and pore


Guard cells photosynthesize to create energy for opening


Subsidiary cells are specialized epidermal cells


Grass-Like Stomata


A-epidermal cells


B- Radially arranged cellulose microfibrils


C- Guard Cells


D- Pore


E- Subsidiary Cells


F- Stomatal complex

Kidney shaped stoma (dicots and non-grass monocots)


A- epidermal cells


B- Radially arranged cellulose microfibrils


C- Guard cells


D- Pore


Subsidiary cells are often absent!

Transpiration Ratio

Amount of H2O transpired divided by amount of CO2 assimilated by photosynthesis




C3 plants- 400


C4 Plants- 150


CAM Plants- 50

Water Use Efficiency

1/Transpiration Ratio


Bigger number=more efficient plant


C3 plants- 0.0025


C4 plants- 0.006


CAM Plants- 0.02

What causes large ratio of H2O efflux to CO2 influx?

Concentration gradient driving H2O loss is 50 times larger


CO2 diffuses 1.6 times slower through air


CO2 must cross membranes before assimilation

Essential Element


(definition)

Component in the structure or metabolism of a plant, or whose absence causes severe abnormalities in plant growth, development and reproduction

Macronutrients


(definition, list)

According to relative concentration


Nitrogen, potassium, calcium, magnesium, phosphorus, sulfur, silicon

Micronutrients


(definition, list)

According to relative concentration


Chlorine, iron, boron, manganese, sodium, zinc, copper, nickel and molybdenum

Classification by Biochemical function

Group 1- carbon compounds


Group 2-Energy storage or structural integrity


Group 3-Ionic form nutrients


Group 4-Redox reaction nutrients

Biochemical classification Group 1


(Definition, list)

Carbon compounds


N- amino acids, proteins, etc


S- amino acids, proteins, oils. Chlorophyll formation, develop and activate certain enzymes and vitamins

Biochemical Classification Group 2


(definition, list)

Every storage or structural integrity


P-Phospholipids and ATP


Si-Cell wall


B-Cel wall. Cell elongation and nucleic acid metabolism

Biochemical Classification Group 3


(definition, list)

Ionic Form


K+ Cofactor and cell turgor and cell electroneutrality


Ca+ middle lamella of cell walls. Cofactor for hydrolysis of ATP and phospholipids. Messenger metabolic regulation


Mg Chlorophyll molecule


Cl- required for photosynthetic reaction involved in O2 evolution


Zn alcohol dehydrogenase, and other ases


Na+ Phosphoenolypyruvate regeneration in C4 and CAM plants

Biochemical Classification Group 4


(definition, list)

Redox Reactions


Fe- cytochromes and nonhdme iron proteins in photosynthesis. N2 fixation and respiration


Mn- photosynthesis O2 evolution


Cu- Ascorbic acid oxidase, helps with photosynthesis


Ni- constituent of urease


Mo- constituent of nitrogenase, nitrate reductase and xanthine dehydrogenase

Hoagland Solution


(definition, use in experiments)

Solution created for plant growth in systems. Provides all necessary nutrients for growth. Can be adjusted as needed

Iron chelation


(how it happens)

Iron bonds to DTPA with ionic forces to make it accessible to plants. Inside the plant it is chelated with organic compounds like citric acid

Deficiency Symptoms

Difficult to diagnose. occurs with improper nutrients. Mobile and immobile nutrients.



Mobile Elements


(definition, list)

Nitrogen, potassium, magnesium, phosphorus, chlorine, sodium, zinc, molybdenum


Symptoms appear on older leaves first- move nutrients to new leaves

Immobile Elements


(definition, list)

Calcium, sulfur, iron, Boron, copper


Symptoms appear on younger leaves first- nutrients can't move around plant

Soil analysis

Chemical determination of nutrient content of soil sample from root zone

Three nutrient zones

Deficiency, adequate, toxic


Must reach critical concentration or plant does very poorly. Also hard to recover from toxicity

Explain the figure
Explain the figure

pH and availability of nutrient elements in soils


Redbox is area where all the nutrients are available. pH between 5.5-6.5 is ideal for all nutrient uptake




Fungi like acidic soils and bacteria like alkaline soils which is important for nutrient uptake

Mineralization

Breakdown of organic compounds into nutrient elements by soil microorganisms

How do roots sense the environment?


(list)

Gravitropism (gravity), thigmotropism (touch), chemotropism (chemicals) and hydrotropism (water)

How many roots does a plant need?

Not that many. They can sufficiently supply nutrients to a plant as long as there are nutrients to be found

Cation exchange


(what and why?)

Replacement of mineral cations adsorbed to the surface of a soil particle


provides a nutrient reserve available to plant roots

Cation exchange capacity (CEC)

Degree to which a soil can adsorb and exchange ions and depends on soil type


Light colored sands- 3-5 men/100g soil


Organic soils- 50-100 men/100g soil



Longitudinal section of the apical region of root


(parts)

Maturation zone- root hairs absorb water and solutes, xylem translocates water and solutes


Elongation zone- 0.7-1.5 mm from apex, cells elongate rapidly


Meristematic zone- cells divide in both directions (base and apex)

Mucigel

Like gelatin. Helps protect root tip as it moves through dirt. Helps with nutrient absorption and microbes

Nutrient Depletion Zone

Rate of nutrient uptake exceeds rate of replacement by bulk flow and diffusion

Arbuscular Mycorrhizal Symbiosis and land plant evolution

AM symbiosis around for >400MYA


Probably helped make the transition to land


Not all plants can make this association

Ectomycorrhiza


(definition)

Outside the roots. Trees and shrubs. Doesn't penetrate cells, but can go between cells.




Often gymnosperms


Often woody plants

Endomycorrhiza


(definition)

Penetrates cells and goes intercellular


Form arbuscules near vascular tissue

Arbuscular mycorrhizae


(what is it)

Arbusculum= small tree


mycos= fungus




Glomeromycota fungus


80% of land plants have it


Obligate symbionts


AM gives plants phosphate and nitrogen


AM fungus gets ~20% of photosynthetic carbon



Arum-type AM

Forms dichotomously (two) branched arbuscules


Travels between cells in intercellular space

Paris-type AM

Form coil-like branched arbuscules


Travels between cells by piercing cell membranes

Development of AM Symbiosis

Spore attaches to root- has enough carbon to germinate


-will die without root


Plants break down vacuole into small compartments so they can hold arbuscule

Label the AM Symbiosis

Label the AM Symbiosis

A-vesicle


B-arbuscules


C-appressorium


D-external hyphae


E-spore

Label the arbuscule

Label the arbuscule

A-cortical cell


B-plasma membrane


C-peri-arbuscular membrane (proteins for nutrient exchange)


D-peri-arbuscular space


E-arbuscule cell wall


A2- arbuscule membrane

Myc factor

"i'm a friendly fungus" chemical


causes calcium spiking in cell with changes gene expression to let AM in cell

Strigolactone

Induce spore germination for fungus- good if its AM fungus


Also causes germination of parasitic seeds, which is bad- seeds 'eavesdrop' on signal


Increased production when soil nutrients are low, which causes more root growth



Hyphopodium

Attaches fungus to root and prepares it for root penetration




Formation of PPA

Pre-penetration apparatus


Breech in cytoplasm that is made of cytoskeleton pieces that guide the fungus into the cell

Fungal penetration


(how it happens)

Fungi hypha enter the PPA, which guides fungus toward inner cortex cells


Fungus enters apoplast (area between cells near inner cortex)


form PPA like structures in inner cortex cells and then make arbuscules

Explain what is happening?

Explain what is happening?

A- mineral nutrients P, N, Zn, and S are leaving the fungus and moving into the plant


B- sugars, which provide carbon are leaving the plant and going to the fungus.




There are proteins to help nutrients move across cell membranes



Efflux

'flowing out'


material leaving a cell

Phosphate transport in Mycorrhizal plants

-Hyphae grow beyond P depletion zone and bring back P that the plant couldn't reach


-Phosphate can enter the cell in two ways- plant from soil, or mycorrhizae from soil to plant


-P is actively transported across the cell membrane in synport- protein that does this is ESSENTIAL for symbiosis

Explain the figure

Explain the figure

Initially strigolactone and salicylic acid increase. Salicylic acid is plant defense and strigolactone stimulates fungus development




Then strigolactone and salicylic acid decrease and jasmonates increase. The plant doesn't need to cause germination or protect itself from the fungus, but jasmonates help protect the plant from insects

Explain the figure

Explain the figure

In plants with no AMF- parasitic plants grow, insects attack the roots and shoots, there are lots of diseases




In plants with AMF- plant growth, repel insects at roots, stop parasitic plants, prime defenses against other nasty things. Plant attracts good insects. Also attract phloem feeders and fungal/viral biotrophs

Photosynthesis overall reaction

6CO2+6H2O+light energy---> C6H12O6+6O2

Nature of light

Wave and particle


Wavelength- distance between crests


Frequency- number of wave crests


Light is a photon


Quantum is the energy of a photon

Amount of energy in a photon

E=hv




Energy= planck's constant x frequency

Electromagnetic spectrum

Purple, blue, green, yellow, red


400----------------------------------700

What happens when molecule absorbs a photon?

molecule in ground state is raised to excited state with more energy. Will fall back to ground state after it passes energy to next molecule

Absorption spectrum of chlorophyll

Absorbs in the purple and blue, and red


reflects green

Label the chloroplast

Label the chloroplast

A- Grana lamella (stacked thylakoids)


B- Thylakoid


C- Stroma lamella


D- Inter membrane space


E- Outer envelope


F- Stroma lamella


G- Granum (stack of thylakoids)


H- Thylakoid lumen


I- Thylakoid


J- Inner envelope

What is a pigment?


(definition)

substance that absorbs light


Plants have a mixture of pigments to increase absorbed light

Chlorophyll

Chlorophyll a and b most abundant in plant


light absorption, energy transfer, electron transfer


Chlorophyll a- has methyl group and magnesium


Chlorophyll b- has aldehyde and magnesium

Explain the image

Explain the image

A shows how the molecule drops energy levels. First heat is emitted, then light is emitted for the final drop to the ground state. Spectra absorption happens at the same place

Action spectrum


(definition)

Represents the magnitude of a response of a biological system to light as a function of wavelength

Antenna Complex


(form, function, location in cell)

Majority of pigments serve in it. Collect light and transfer energy to a reaction center complex, where redox reactions lead to long-term energy storage take place


PSI and PSII

Reduction reaction


(definition)

gain of electrons, hydrogen or the loss of oxygen


decrease charge of moledule

Oxidation reaction


(definition)

loss of electrons, hydrogens, or gain of oxygen


increase charge of molecule

NAD+ and NADP+


(definition)

NAD+ nicotinamide adenine dinucleotide


NADP+ nicotinamide adenine dinucleotide phosphate




Two strong oxidizing agents- oxidize materials they react with

NADP and NADPH


(definition)

two strong reducing agents, with give the hydrogens

Redox potential


(definition)

tendency to accept or donate electrons

Light/Thylakoid reactions


(input, output, location)

Input- H2O, NADP+, ADP


Output- O2, NADPH, ATP




In the thylakoid

Light-independent reactions


(input, output, location)

Input- ATP, NADPH, CO2


Output- ADP, NADP+, Carbohydrates




In the stroma

Quantum Yield


(definition)

Number of photochemical products/ total number of quanta absorbed

Photosynthesis and red light


(what types of red light?)

Need both red and far red light


photosynthesis works best when light is absorbed by chlorophyll, not accessory pigments

Describe image

Describe image

Z scheme of photosynthesis.


-Red light is absorbed by PSII and produces a strong oxidant and a weak reductant


-Far red light is absorbed by PSI and produces a weak oxidant and strong reductant


-Strong oxidant from PSII oxidizes H2O, while strong reductant produced by PSI reduces NADP+


Light Reaction!

Explain the image

Explain the image

-How energy flows through pigments to the reaction center


-95-99% of photons absorbed by antenna pigments have their energy transferred to the reaction center


-Pigments closer to the reaction center are lower in energy to create a energy gradient so it flows to the center

Fluorescence resonance energy transfer


(definition)

physical mechanism by which the excitation energy is transferred from one molecule to another

Photosystems I and II


(location, connector, ratio)

PSII is in grana lamellae and PSI is in the stroma lamellae and edges of grana lamellae




Cytochrome b6f complex connects photosystems




PSII to PSI ratio is usually 1.5:1

Four major protein complexes in light reactions

Photosystem 2, cytochrome b6f complex, photosystem 1, ATP synthase

Photosystem 2 reaction center


(parts, proteins)

Multisubunit pigment-protein


Two complete reaction centers and some antenna complexes


Core reaction center two membrane proteins D1 and D2


Primary donor chlorophyll, carotenoids, pheophytins, and plastoquinones are bound to D1 and D2

PS2 Water oxidation


(what, cofactor)

PS2 oxidizes H2O and releases protons into lumen


Occurs in Oxygen evolving complex


Manganese is essential cofactor


But we don't know why

Election acceptors from PS2


(pathway through cell)

Pheophytin, a chlorophyll with two hydrogens instead of Mg, acts as first acceptor


To Plastoquinones PQa and PQb are bound to reaction center and receive electrons from pheophytin


PQB is reduced to PQB-2, then takes two protons from stroma, making PQH2, fully reduced


PQH2 disassociates from reaction center and transfers electrons to cytochrome b6f

Cytochrome b6f


(parts!)

Large, multisubunit protein


Two b-type hemes and one c-type hemes


Risk iron-sulfur protein


Q cycle for proton flow


PQH2 is oxidized and one of the two electrons is based down linear process to PS1, other electron enters cyclic cycle

Linear Q cycle


(pathway)

First PQH2 is oxidized, so two H+ are released into lumen.


One electron is passed to Reiske protein, then to Cyt f, then to plastocyanin


Second is passed to Cytb, then heme c, then the PQ is reduced once

Cyclic Q cycle


(pathway)

Second PQH2 is oxidized, so two H+ are released into lumen.


One electron is passed to reissue, then cut f then plastocyanin


Second is passed to cytB, then heme C, then to the once reduce PQ. This fully reduces the PQ. It takes two H+ from the stroma which brings more into the lumen!

Mobile Carriers between PS2 and PS1

Plastoquinone and plastocyanin

Plastocyanin


(definition)

Passes electrons from cytochrome b6f to P700

Photosystem 1


(parts)

Large multisubunit complex


Core antenna consisting of about 100 chlorophyll is integral part of PS1 reaction center


Core antenna and P700 are bound to two proteins PsaA and PsaB


Core antenna pigments surround electron transfer cofactors

Electron acceptors in PS1


(pathway)

A0, pqhylloquinone


Fe-S centers are also electron acceptors


Order- A0, A1, FeSx, FeSa, FeSb to Ferredoxin (fd) to ferredoxin NADP reductase (FNR) (do not need to memorize!)

How electrons move through photosystem 1


(pathway)

From PsaA to PsaB to Ferrodoxin to ferredoxin NADP reductase

Photophyosphorylation


(what is this?)

Light-dependent ATP synthesis


Requires electron flow

Chemiosmotic mechanism


(definition)

Difference in ion concentration and electric potential across membrane are sources of energy to be used. Lower concentration in stroma makes energy flow out of cell through ATP synthase

ATP synthase


(parts, what it does)

Found only in stroma lamella and at edges of grana stacks


Consists of a hydrophobic membrane bound protein (CF0) and a portion that sticks out (CF1)


CF1 rotates to make ATP, and one rotation makes three ATP

Repair and regulation of photosynthetic machinery


(definition, three carotenoids used)

Too much light can lead to production of toxic O2 species.


Carotenoids can take excess energy and protect cell from making toxic O2.




Violaxanthin-low


Antheraxanthin- intermediate


Zeaxanthin- high light




PS2 is most affected because it produces 02 specie

Photo inhibition


(definition, what it does)

Complex set of molecular processes the inhibit photosynthesis in excess light




Reversible in early stages, but later on not


Prolonged inhibition requires total replacement of PS2


D1 protein most targed

Plastocyanin creation


(location)

Made in cytoplasm by nuclear DNA and imported into chloroplast.

Calvin-benson cycle


(input, output, location)

Input- ATP, NADPH, CO2


Output- ADP, NADP+, Carbohydrates




Stroma

Stages of Calvin-benson cycle


(list)

Carboxylation, reduction and regeneration

Ribulose 1, 5-bisphosphate carboxylase/oxygenase (Rubisco)


(function, metal ion)

Slow enzyme that breaks RuBP into 3-phophoglycerate




Needs Mg2+ to function

Carboxylation step of Calvin-benson cycle


(molecules, enzymes, reactions)

3 RuBP (5 carbon sugar) combine with 3CO2 and 3H2O in Rubisco to make 6 3-phosphoglycerate molecules

Reduction step of Calvin-benson cycle

(molecules, enzymes, reactions)


6 3-PGA are phosphorylated by 6 ATP molecules in 3-phosphoglycerate kinase to make 1,3-bisphosphoglycerate




6 NADPH +6 H+ interact with 1,3-bisphosphoglycerate in NADA-glyceraldehyde 3 phosphate dehydrogenase to make Gylceraldehyde 3-phosphate (G3P)

Regeneration step of Calvin-benson cycle


(molecules, enzymes, reactions)

1 G3P is made into starch and sucrose




5 G3P react with 3 ATP to make 3 RuBP over 10 different enzymatic reactions


3 RuBP return to start of cycle

Photorespiration


(definition, location)

When Rubisco uses O2 instead of CO2 and forms a toxic byproduct and how it gets rid of the byproduct




Takes place in Chloroplast, peroxisome, and mitochondria

Calvin-benson cycle reactions net inputs and products

3CO2 + 5H2O + 6NADPH + 9ATP ----->


Glyceraldehyde 3-phosphate + 6(NADP+) + 3(H+) + 9ADP + 8Pi

Number of ATP to NADPH needed for fixation of CO2 in calvin-benson reaction

3:2

Initial mitochondrial steps of photorespiration

Rubisco uses 2 O2 instead of 2 CO2 and 2 RuBP to make 2 3-Phosphoglycerate and 2 phosphoglycolate (BAD!)




2 Phosphoglycolate and 2 water turn into 2 glycolate

Initial peroxisome steps of photorespiration

2 Glycolate interacts with 2 O2 to make 2 H2O (which is then split) and 2 Glyoxylate




2 Glyoxylate are turned into 2 Glycine

Mitochondrial steps of photorespiration

2 Glycine and NAD+ work with H2O to make CO2, NH3+, NADH and Serine (nitrogen cycling)

Final peroxisome steps of photorespiration

Serine is turned into Hydroxypyruvate




Hydroxypyruvate interacts with NADH to make NAD+ and Glycerate

Final chloroplast steps of photorespiration

Glycerinate and ATP make 3-phosphoglycerate and ADP which can return to the Calvin-benson cycle

Recovery of Fd (for calvin-benson cycle)


(location, enzyme, ingredients)

In stroma of chloroplast


Uses GOGAT


Reduced Fd recovers glutamine to glutamate and Fd becomes oxidized in process to return to CB cycle

Photorespiration and photosynthesis protection

Minimizes photo inhibition


Prevents over reduction of photosynthetic chain by taking excess reducing equivalents


Make H2O2, which is a stress signaling molecule

Types of Photosynthesis


(list)

C3, C4, CAM

C4 Carbon cycle


(why does it exist?)

Reduce water loss by minimizing photorespiration




Add-ons to separate uptake of CO2 and supply to Rubisco to prevent oxygenase from working

C4 Leaf anatomy

Mostly spongy mesophyll


Large and well developed bundle sheath


Almost no airspaces in mesophyll

C4 plants


(what type of environment do they do best in?)

Hot and high illumination because they can make a high concentration of CO2 around bundle sheath cells

Label the parts

Label the parts

A- CO2


B- PEP carboxylase


C- Oxaloacetate


D- Malate


E- CO2


F- Calvin Cycle


G- Sugar


H- Pyruvate


I- ATP


J- ADP


K- PEP


L- Mesophyll Cell


M- Bundle-sheath cell


N- Vascular tissue

C4 Photosynthetic Carbon cycle


(pathway)

PEP Carboxylase catalyzes HCo3- with PEP to make Oxaloacetate


Oxaloacetate is converted by NADP-malate dehydrogenase to malate


Malate flows to bundle sheath cells and NAD(P)-malic enzyme released CO2 from malate, creating pyruvate


CO2 goes to calvin cycle


Pyruvate flows back to mesophyll, reacts with pyruvate phosphate dikinase and ATP to regenerate PEP

Amount of ATP used in C4 carbon cycle

Two ATP per mole of fixed CO2

C4 in single cells

C4 can be in one cell


Depends on diffusion gradients, with different types of chloroplasts in different parts of cell

CAM Plants


(where do they live?)

Arid environments, good at concentrating CO2 at site of rubisco to save water

CAM plants and how they minimize water loss

thick cuticles, large vacuoles, stomata with small apertures, tight packing of mesophyll cells

24-hour CAM cycle with 4 phases


(Phase times and enzyme activity)

Phase I: night


Phase II: early morning- increase Rubisco activity


Phase III: daytime


Phase IV: later afternoon- PEPCase activity increase

CAM Dark cycle

Stomata open for CO2 intake


CO2 combines with H2O in cell to make HCO3-


PEP Carboxylase takes HCO3- and PEP to male oxaloacetate


OAA reduced with NADH by NAD-malate dehydrogenase to make malate and NAD+


Malic acid (low pH in vacuole) stored in vacuole for Light cycle

CAM Light cycle

Stomata closed no CO2 uptake


Malic acid made back into malate


Malate decarboxylase makes malate release CO2 for Calvin cycle


Resulting pyruvate turned into starch



Obligate vs Facultative CAM

Obligate must always use CAM


Facultative use C3 when safe but otherwise use CAM. Can change by leaf

Sucrose vs Starch


(Location in plant, use)

Sucrose: translocated in phloem and used for energy. Flows from source to sink tissues. Created in cytosol




Starch: end product in place it needs to stay i.e. tuber or chloroplast to be used the next day. stored in granules in chloroplast. Long term storage. made in chloroplast

Partitioning of Photosynthates


(overnight? what happens in sink leaves?)

Overnight, CO2 assimilation stops, but plant still respires. Uses stored starch to make sugars for energy




Low sugar in sink leaves stimulates photosynthesis and mobilizes carbs from storage organs