Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
44 Cards in this Set
- Front
- Back
|
where is the site for G protein interaction on GPCR's?
|
at the intracellular C-terminus and C3 loop
|
|
|
where are the ligands recognized in GPCR's?
|
at the 3D pocket made by the extracellular N-terminus and loop domains
|
|
|
what is wrong in familial male precocious disease?
|
autosomal dominant
aspartate to glycine in H6 of LH GPCR always active |
|
|
what is wrong in thyroid adenoma?
|
hyperthyroidism
two mutations in third cytoplasmic loop, aspartic acid to glycine and alanine to isoleucine independent activation |
|
|
how are subfamilies of G proteins categorized?
|
by amino acid sequence of G alpha subunit
|
|
|
how many subunits do G proteins have
|
3
alpha, beta and gamma |
|
|
what are the actions of the G alpha subunit?
|
binds to activating sequence of GPCR
binds and hydrolyzes GTP to GDP |
|
|
what turns off the G protein signaling pathway?
|
GTPase activity of the G alpha subunit
GTP to GDP |
|
|
effect of cholera toxin on G protein?
|
inhibits GTPase, persistent activation
|
|
|
effect of pertussis toxin on G protein?
|
blocks the interaction of subunit with receptor
|
|
|
what cytosolic proteins contain SH2 domains?
|
PI3-kinase
PLC-gamma GTPase-activating protein |
|
|
what is the ligand for soluble guanylyl cyclases?
|
NO
|
|
|
what is the ligand for membrane-bound guanylyl cyclases?
|
ANF
|
|
|
what site binds ligands in the hormone receptor?
|
C-terminus
|
|
|
what site controls gene transcription in the hormone receptor?
|
N-terminus
|
|
|
what do non-hormone receptors do?
|
respond to lipid levels in the cell
|
|
|
examples of steroid hormone receptors?
|
sex hormone receptor
glucocorticoid receptor vitamin D receptor thyroid hormone receptor retinoid receptor |
|
|
examples of non-hormone receptors?
|
PPARgamma
LXR SXR |
|
|
how do non-hormone receptors monitor lipid levels?
|
control transcription of genes for cytochrome P450(inactivate lipids), intracellular lipid binding proteins(buffer and transport), and ATP binding cassette transporters( transport)
|
|
|
what cytosolic proteins contain SH2 domains?
|
PI3-kinase
PLC-gamma GTPase-activating protein |
|
|
what is the ligand for soluble guanylyl cyclases?
|
NO
|
|
|
what is the ligand for membrane-bound guanylyl cyclases?
|
ANF
|
|
|
what site binds ligands in the hormone receptor?
|
C-terminus
|
|
|
what site controls gene transcription in the hormone receptor?
|
N-terminus
|
|
|
what do non-hormone receptors do?
|
respond to lipid levels in the cell
|
|
|
examples of steroid hormone receptors?
|
sex hormone receptor
glucocorticoid receptor vitamin D receptor thyroid hormone receptor retinoid receptor |
|
|
examples of non-hormone receptors?
|
PPARgamma
LXR SXR |
|
|
how do non-hormone receptors monitor lipid levels?
|
control transcription of genes for cytochrome P450(inactivate lipids), intracellular lipid binding proteins(buffer and transport), and ATP binding cassette transporters( transport)
|
|
|
what segment of the voltage channel recognizes the ion?
|
P segment
|
|
|
where is the voltage sensor located?
|
cytosolic side
|
|
|
where are the recognition sites in ligand gated channels?
|
alpha subunits between alpha gamma and alpha delta
|
|
|
what do glucose carriers not transport?
|
sucrose and lactose(disaccharides)
|
|
|
what is the initial step of K+ transport into the cell?
|
dephosphorylation
|
|
|
where are Ca2+ ATPases located?
|
plasma membrane and ER
|
|
|
what transporters are driven by the Na+ gradient?
|
Na+-glucose symporter
Ca/Na antiporter |
|
|
what is a common competitive antagonist for ligand-gated acetylcholine receptors?
|
curare
|
|
|
what is the mechanism of local anesthetic?
|
binding to phe and tyr residues of the Na channel in S6 of the fourth subunit
|
|
|
how do anti-arrhythmics function?
|
block open channels, reduce excitability
|
|
|
how do anti-epileptics work?
|
block inactivated channels, slows recovery rate, limits repetitive firing
|
|
|
what is used in heart failure?
|
cardiac glycosides(digitoxin)
bind to and block the Na/K ATPase increase intracellular Ca increase contraction |
|
|
what is cochicine used for?
|
killing abnormally rapidly dividing cells by binding to tubulin and preventing microtubule formation
|
|
|
what is taxol used for?
|
binds to and stabilizes microtubules arresting cells in mitosis
|
|
|
what are benzimidazole derivatives used for?
|
binding to parasitic tubulin
|
|
|
what is cochicine used to treat?
|
gouty arthritis
inhibits leukocyte migration and release of inflammatory mediators |
