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18 Cards in this Set
- Front
- Back
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C- elegans strains can be frozen
Hermaphrodites have --- somatic cells Male has --- somatic cells How many neurons? |
959
1031 302 |
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when hermaphrodites self fertilize they produce ----
when they mate with males.. they produce --- |
hermaphrodites
half hermaphrodites, half males |
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Wild type worms are very active and move ---
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sinusoidally
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bli-1 c.elegans has what distinctive about it?
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larger clear area on the side of the worm (blister in the cuticle)
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dpy - 10 (dumpy worms) c. elegans are ---- and wider than wild type
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shorter and wider
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rol-6, roller worms have --- bodies and roll in circles
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twisted,
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Unc-22 what are some characteristics?
shape? how do they move? |
lie still, outstretched not s-shaped, and they twitch
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RNAi amplification/transitive signal
Primary siRNA bind to it complementary mRNA sequence and recruited ---- to the region. ---- will generate ---- siRNAs that are of ---- orientation. This will base pair to the mRNA sequence located ----- 5' from where the primary siRNA is bound. Both primary and secondary siRNAs lead to ----- gene silencing. |
RdBP , RdBP, secondary, antisense, upstream, specific
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siRNAs are transported from cell to cell via ---- proteins called...
Transport is active or passive? Found in what kind of tissues? No present in other worm species |
membrane, SID (systemic RNAi Defective)
passive non-neuronal |
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C. elegans can be used for?
3 things |
development
cell fate control gene/protein function screens (RNAi) |
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xenopus laevis is a
used to study --- eggs used to study --- and --- regulation |
frog
developement cell cycle, apoptosis |
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Nude mice have no -- cells
they do not reject ----- good for studying ------ |
T
xenografts tumorigenesis |
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Danio rerio is a
used for ----- studies have small, transparent --- |
fish
developemental eggs |
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Zea mays is ....
Oryza sativa is... |
maize
rice |
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Arabidothopsis thaliana has a small genome of --- base pairs
Can it be transfected? |
150 million
yes also has small seeds, lots of variation, lots of seed, and short life cycle |
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Which organism has the highest number of protein coding genes?
orthologs to human genes? Which have the least orthologs and least protein coding genes? |
aradiopsis thaliana
M.musculus e.coli |
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Sort the number of protein coding genes from low to high in model organism
elegans cerevisae musculus coli thaliana sapiens melanogaster |
coli
cerevisae elegans melanogaster musculus sapiens thaliana |
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sort the # of orthologs of human genes of model orgs from low to high
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coli
cerevisae thaliana elegans melangogaster musculus |
