Parasitology Diagnosis Exam 2

Front 1

Back 1

Plasmodium vivax trophozoite/ring form
- One chromatin dot with ring on it
- Schuffner's dots in cytoplasm
- Yellow-brown material pigment (malarial pigment)

Front 2

Back 2

Plasmodium vivax schizont
- 12-24 merozoites

Front 3

Back 3

Plasmodium vivax gametocyte
- Round with "granules"

Front 4

Back 4

Plasmodium falciparum trophozoite
- Headphone ring with to chromatin dots
- Mauer's clefts (dark blue staining of headphones)
- Applique form: ring on membrane of RBC

Front 5

Back 5

Plasmodium falciparum gametocyte
- Cigar shaped (sausage or crescent)

Front 6

Back 6

Plasmodium ovale comet form
- Chromatin dot with comet shaped ring

Front 7

Back 7

Plasmodium ovale trophozoite
- Shuffner's dots
- Oval shaped
- Ragged edges

Front 8

Back 8

Plasmodium ovale schizont
- Oval shaped
- 4-12 merozoites clustered around malarial pigment

Front 9

Back 9

Plasmodium malariae trophozoite
- elongated "band" form in RBC
- Ziemann's stippling (no malarial pigment)

Front 10

Back 10

Plasmodium malariae schizont
- 6-12 merozoites
- Daisy appearance
- Large nuclei with malarial pigment

Front 11

Back 11

Plasmodium malariae gametocyte (left) and ring (right)

Front 12

Back 12

Babesia sp. ring form
- Extracellular rings distinguish from malaria
- Maltese cross with 4 rings (looks like a chromosome)

Front 13

Back 13

Toxoplasma gondii tachyzoite

Front 14

Back 14

Toxoplasma gondii bradyzoite

Front 15

Back 15

Enterobius vermicularis (pinworm) ova
- Oval, flattened on one side
- Thich, colorless shell
- 50-60 micrometers by 20-30 micrometers

Front 16

Back 16

Eneterobius vermicularis (pinworm) adult
- Long, pointy tail
- 7-13 mm female (males smaller)

Front 17

Back 17

Fertilized (left) and Decorticated (right) Ascaris lumbricoides (Large intestinal roundworm) ova
- Fertilized ovum
* Rounded
* Yellow-brown mammillated shell
- Decorticated ovum
* Same but without mammillated shell

Front 18

Back 18

Ascaris lumbricoides (Large intestinal roundworm) unfertilized (left) and fertilized (right) ova
- Unfertilized ovum
* Elongated
* Yellow-brown mammillated shell
- Fertilized ovum
* Rounded
* Yellow-brown mammillated shell

Front 19

Back 19

Anisakis sp. endoscopy
- Pink and tightly coiled larvae

Front 20

Back 20

Trichuris trichiura (whipworm) ova
- Barrel shaped
- Thock smooth shell
- Clear "plugs" at each end
- 45-55 micrometers by 20-23 micrometers

Front 21

Back 21

Hookworm primitive embryo ova
- 55-75 micrometers by 35-40 micrometers
- Thin, clear, smooth colorless shell
- Clear space between eggshell and embryo

Front 22

Back 22

Hookworm embryonated ovum
- Embryo growing inside
- Same as primitive embryo ovum

Front 23

Back 23

Hookworm rhabditiform larvae
- Long buccal cavity
- Small genital primordium

Front 24

Back 24

Ancylostoma duodenale (Old world hookworm) (left)
- 4 teeth for mouth
Necator americanus (New world hookworm) (right)
- 4 cutting plates for mouth

Front 25

Back 25

Strongyloides tercoralis (threadworm) rhabditiform larvae
- Short buccal cavity
- Large genital primordium

Front 26

Back 26

Wuchereria boncrofti microfilariae
- Sheathed
- 245-300 micrometers
- Nuclei DO NOT extend to tip of tail

Front 27

Back 27

Brugia malayi microfilariae
- Sheathed
- 200-275 micrometers length
- Nuclei extend to tip of tail
- Two terminal nuclei may be seen separate from the other nuclei at the end of one tail

Front 28

Back 28

Loa loa microfilariae
- Sheathed
- 250-300 micrometers
- Body nuclei continuous to tip of tail

Front 29

Back 29

Onchocerca volvulus microfilariae in skin nodule
- Only seen from a skin biopsy where infection is

Front 30

Back 30

Mansonella spp.
- Unsheathed
- M. perstans
* Seen in blood
* No shepherd's hook
- M. ozzardi
* Seen in blood
* Shepherd's hook at end of tail
- M. streptocerca
* Seen in skin biopsy
* Shepherd's hook seen in tail

Front 31

Back 31

Diphyllobothrium latum ovum
- Operculated egg
- Boss (knob) at opposite end of operculum
- Develop embryo when shed

Front 32

Back 32

Taenia sp. ovum
- Radially striated shell
- Onchosphere with 6 hooklets in 3 pairs (hexacanth embryo)
- INFECTIVE (cysticercosis)

Front 33

Back 33

Taenia scolium (pork tapeworm) proglottid
- 7-14 branches on each side

Front 34

Back 34

Taenia scolium (pork tapeworm) scolex
- 4 suckers
- 2 rows of hooks

Front 35

Back 35

Taenia saginata (Beef tapeworm) proglottid
- 15-25 uterine branches on each side

Front 36

Back 36

Taenia saginata scolex
- 4 suckers
- NO TEETH

Front 37

Back 37

Hymenolepsis nana (dwarf tapeworm) ovum
- Inner envelope
- 6 hooked oncospheres contained inside
- INFECTIVE

Front 38

Back 38

Hymenolepsis diminuta (rat tapeworm) ovum
- Appear like nana but are LARGER (60-80 micrometers)

Front 39

Back 39

Dipylidium caninum ovum packet
- Membrane-bound sacks with 5-15 eggs with oncosphere embryos

Front 40

Back 40

Dipylidium caninum proglottid
- 2 genital pores
- Resemble maggots when passed

Front 41

Back 41

Echinoccocus granulosus (hydatid tapeworm) hydatid cyst
- Laminated outer layer
- Inner germinal center (daughter cysts and brood capsules)
- Fluid with hydatid sand (loose germinal tissue with inverted scolices)

Front 42

Back 42

Echinococcus multilocularis (fox/coyote tapeworm) cyst