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42 Cards in this Set
- Front
- Back
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McMaster Egg Counting Technique |
Quantitative analysis |
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McMaster Egg Counting Technique |
uses a counting chamber which enables a known volume of fecal suspension to be examined microscopically |
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Eggs Per Gram |
unit used to describe the parasitic burden |
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Simple Fecal Smear |
Usually done in clinic. Disadvantage: too few eggs may mislead diagnosis |
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Qualitative |
Check quality of eggs •sugar floatation |
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Pseudoparasites |
Looks like parasite that may confuse and mislead. This may or may not be present in a sample. |
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spurious |
parasites that are not meant to be present but is just a product of predation/ cannibalism/ prophagy |
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4 |
1st step Weigh ________ grams of feces and place into a container |
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56ml (28ml) |
Add __________ of your chosen flotation fluid |
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fork tongue depressor spatula |
stir the contents of the beaker thoroughly with ___________ |
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Tea strainer Double layer of cheese cloth Dental napkin |
4. Filter the fecal suspension through a _________, or _________, or _________ into the second container |
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Pasteur pipette |
Stir the filtrate in containee two with a _________ |
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T |
T/F using the pipette, withdraw a sub-sample as the filtrate is being stirred |
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True |
T/F 6. Stir fluid and fill first compartment of the McMaster counting chamber with the sub-sample. Stir fluid again and fill second chamber with another sub-sample |
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5 minutes |
7. Allo the counting chamber to stand for ________ minutes |
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this allows the eggs to float to the surface and the debris to go to the bottom of the chamber |
It is important to leave the chamber to stand because _________ |
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10x10 magnification |
examine the sub-sample of the filtrate under what magnification? |
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(a + b) x 50 |
formula for counting eggs using McMaster Egg Counting Technique |
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60ml fecal sol'n/0.3 ml= 200/4 grams= 50@ |
where did we derive the constant value of 50 |
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low |
fewer than 100 epg |
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high |
higher than han 500 epg |
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moderate |
500 epg |
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similar morphological features |
take note that you should only count all the eggs with ___________ |
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distort or destroy eggs |
do not delay reading the count beyond the recommended time as the flotation fluid may ____________ |
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true |
T/F it is advisable to only process a few samples at a time |
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preserve susing 10% formalin refrigerate |
ways to preserve fecal sample |
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Family Fasciolidae |
body is large and leaf-like ; with suckers close together at the anterior end |
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Family Fasciolidae |
parasites of the liver and bile ducts of herbivorous mammals and man |
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Fasciola Fascioloides Fasciolopsis |
give the genera of family Fasciolidae |
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cattle water buffaloes small ruminants rabbit humans other mammals |
final hosts of fasciola gigantica |
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Lymnaea auricularia rubiginosa Lymnaea philippinensis or Lymnaea viridis |
1st intermediate hosts of fasciola gigantica |
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Liver- immature Bile ducts- mature |
predilection site of fasciola gigantica |
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EGG of fasciola |
yellowish, operculated, 120 um x 90um length, filled with granulated cells |
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Miracidium |
swims in water, with cilia, 3 hours life span |
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sporocyst |
without cilia, with germinated balls |
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5-8 first generation redia |
equivalent of 1 sporocyst |
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redia |
with mouth and digestive organs and eats its way through the snail's tissue, with germinal balls |
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germinal balls |
progenitors of 2nd generation rediae |
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cercaria |
tadpole-like |
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650 cercariae |
equivalent of 1 miracidium |
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cercariae |
Leptocercous/gymnocephalus |
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metacercaria |
encysts in vegetation |
