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32 Cards in this Set
- Front
- Back
- 3rd side (hint)
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trophozte |
_vegetative feeding stage -motile -watery stool -stain: quensel, bufferd methyene blue |
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cyst |
-nonmotile -resistant -formed stool -d antonis, lugols |
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Amoeba generalities |
Encystation except e. Gingivalis Cyst is infectious except e. Gingivalis Have peripheral chromatin |
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Entamoeba histolytica (troph) |
MOT: Ingestion FH : man Ingest rbc 1 nucleus Central karyosome Habitat: L. Intestine Pseudopodia: hyaline finger like |
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Entamoeba histolytica (cyst) |
4 nucleus Sausage shape chromatoidal bar |
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Entamoeba coli (troph) |
Larger than e. Histolytica Eccentric dirty karyosome Non pathogenic Pseudopodia : blunt granular |
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Entamoeba coli(cyst) |
8 Witchbroom chromatoidal bar |
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Other pathogenic intestinal protozoan |
Balamuthia mandrillaris Acanthamoeba Naegleria fowleri |
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Pathology of E.histolytica |
1.intestinal amebiasis -flask shape ulcer -bloody mucoid diarhea -colitis~ infection of colon -amebomas~ granulomas; mistaken for carcinoma 2. Extraintestinal -amebic liver abses ~anchovy sauce appearance -liver lung brain |
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Virulence factor e histolytica (3) |
Lectin- attachment Amebopore- form pore Cysteine proteinase- spread; tiddue destruction |
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Stain for lab diagnosis |
Trichrome with pva Iron hematoxylin (spencer monroe and tompkins miller |
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Laboratiry dx e hisolytica |
Confirmatory: pernanent stained slodes observe charcot leyden crystal |
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Charcot leyden crystal |
Breakdown of eosinophil |
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Other commensal ameba |
Entameba hartmanni Entamoba polecki Iodamoeba butschlii Endolimax nana Entamoeba gingivalis |
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Entamoeba hartmanni cyst |
Same with e. Histolytica |
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Entamoeba polecki cyst |
Pointed or angular ended karysome |
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Iodameba butschlii |
Basket of flower karyosome Large glycogen mass satains with iodine |
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Endolimax nana cyst |
Cross eyed cyst Blot like karyosome |
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Naegleria fowleri |
Ameboflagellate Ameba- lobate psedopodia~limax form Flagellate form Mot: olfactory respiratiry skin sinus IS: Troph Dss: PAM primary amebic meningoencephalitis Amebostomes: food cup Dx: postpartum csf |
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Acanthamoeba |
Mot: skin nose is: troph cyst Troph: acanthapodia~ spiny projection Cyst: double walled Dss: GAE granulomatous amebic encephalitis, Amebic keratitis Dx:brain biopsy, corneal scraping calcoflour white, stain cyst with pas |
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Balamuthia madrillaris |
Mot: skin nose Is: troph cyst Troph: fingerlike broad irregular pseudopodia Cyst: double wall Dss: GAE Dx: not grow with agar, brain biopsy, IF |
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Sappinia pedata |
Encephalitis Troph: binucleated, ingest rbc Stain: giemsa, pas |
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Types of flagellate |
Urogenital, Intestinal flagellate Hemoflagellate |
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Intestinal urogenial flagellate generalities |
-L.intestine except g.lamblia t.vaginalis t.tenax -commensal except g.lamblia t.vaginalis d.fragilis -asexual through binary fision -encystation except trichomonas spp |
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Pathogenic intestinal flagellate |
Giardia lamblia |
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Giardia lamblia generalities |
Reservoir beaver Is cyst Mot ingest cyst low dose 8-10 Dx stage troph cyst |
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Giardia lamblia troph |
Pear or pyriform shape Bilaterally symetrical Appearance old man with glasses Ventral sucking disc 1 for attachment Flagella 8 Median body/parabasal 2 energizing Axostyle support locomotion Motility falling leaf |
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G.lamblia cyst |
Football shape Nucleus 4 Parabasal body Axoneme |
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G.lamblia dss |
Beaver fever Traveller diarhea Gay bowel syndrome Giardiasis |
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G.lamblia pathogenicity |
Ventral sucking disc |
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G.lamblia dx |
Enterotest ~ beale's string test |
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Chilomastix mesnili |
Commensal, l.intestine |
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