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26 Cards in this Set
- Front
- Back
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Light Microscopy |
The use of any kind of microscope that uses visible light to observe soecimens
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Ocular Lens
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Re-magnifies the image formed by the objective lens |
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Objective lenses
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Primary lenses that magnify the speciman
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Diaphragm
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Controls the amount of light entering the condenser
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Coarse adjustment knob
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Raises the stage up and down; use with 4x and 10x objective
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Fine adjustment knob
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Focuses the speciman without moving the stage; use with 40x and 100x objective |
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stage adjustment knobs
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adjusts the (slide) on the stage either horizontally or vertically |
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Total magnification =
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(objective lens)(ocular lens)= |
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Refractive index
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The degree of bending that the light undergoes as it passes through mediums
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Darkfield Microscope
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Light objects are visible against a dark background
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Light reflect off the specimen enters the objective lens |
Darkfield Microscope |
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Phase contrast
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Disc partially blocks direct light allowing for direct and diffracted light to be reinforced. INTERNAL STRUCTURES BECOME MORE SHARPLY DEFINED
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Differential interference contrast micriscopy |
Uses 2 beams of light
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Fluorescence microscopy
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Uses UV light, cells studied may also be stained with fluorescent dye
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Confocal microscopy
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The light illuminates each plane in a specimen to produce a 3-d image
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Electron Microscopy
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Uses electrons instead of light, the shorter wavelength of electrons give greater resolution |
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Which type of microscopy gives the greatest resolution? |
Electron Microscopy
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This provides Ultra-thin sections of specimens |
Transmission Electron Microscopy |
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1,000x-10,000x; resolution 20nm; SURFACE VIEW OF SPECIMEN ONLY |
Scanning Electron Microscopy
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uses various types of probes to examine the surface of a specimen using electric current which does not destroy the organism
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Scanned-Probe Microscopy
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This uses a metal and diamond probe inserted into the specimen, produces a 3-d image of internal structures and can account for the chemical composition and temperature of a specimen |
Atomic Force Microscopy
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SMEAR technique (Robert Koch) |
1.Smear
2. Air Dry 3. Heat Fix 4. Stain |
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Simple stain
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use of a single, basic dye
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differential stain
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uses primary dye and counter-stain to distinguish between cell types or parts
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Gram positive bacteria |
tend to be killed by penicillin and detergents |
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Gram negative bacteria |
tend to be more resistant to antibiotics |
