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10 Cards in this Set
- Front
- Back
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When doing a __________, genomic DNA from a variety of organisms is cut into small fragments, seperated by aragosegel electrophoresis, and then transferred to a membrane. The membrane is then hybridized with another piece of DNA to determine if the probe is from a gene or a non-coding region.
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Zoo Blot
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_______ are used in chemical synthesis of DNA because the chemistry around the phosphate leaves only one phosphate bond available for linking to the previous nucleotide.
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phophoramidite nucleotides
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A _______ emits a flash of light when a radioactive isotope decays, and these light flashes are detected by a photocell and recorded in a _______.
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scintillant, scintillant counter
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The term _______ refers to a piece of DNA that has been labeled with a chemical tag or radioisotope that is used in hybridization experiments to find complementary sequences among a large complex mixture of sequences
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probe
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______ uses a DNA probe to hybridize to either genomic DNA or cDNA separated in a gel, whereas ______ uses a DNA probe to hybridized to mRNA
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southern blotting, northern blotting
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The ______ for a piece of DNA is a the point where half of the strand is melted or single-stranded and the other half still has hydrogen bonds hold the two strands together.
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melting temperature
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Hairpin DNA probes, which have one end with a fluorescent tag and the opposite end with a quenching group, are called______
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nuclear beacons
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X-phos is a ________ because it releases a blue dye when mixed with alkaline phosphatase.
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chromogenic substrate
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In DNA isolation, ______ is added to digest the cell wall, _____ is added to dissolve the cell membrane, and ______ is used to separate heavier cellular components, like DNA, from the digested parts like cell wall and cell membrane pieces.
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lysomes, detergent, centrifugation
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Chemical tags include _______ (a vitamin) that is bound by _______ and _______ (a steroid from foxglove) that is bound by an ___________
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biotin, avidin, digoxigenin
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