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17 Cards in this Set
- Front
- Back
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RecBCD |
-enters at DSB -involved in initiation of DNA recombination and processing -has helicase, exonuclease and endonuclease activity |
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RecA |
-involved in strand invasion, homologous pairing and formation of Holliday-type structure -forms Nucleoprotein Filaments -promotes strand-exchange reaction -performs homology searching -Euk homologs are Rad51 and Dmc1 which both function in meiotic recombination |
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RuvAB |
involved in branch migration in prokaryotes |
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RuvC Resolvase |
-catalyzes the resolution of Holliday Junctions -dimer that clips 2 DNA strands to yield either patch or splice recombinant products -clipping occurs at 5'-(A/T)TT(G/C)-3' |
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Eukaryotic Recombination Proteins |
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RAD52 |
-and BRCA2 are both recombination mediators -it forms a complex with RAD51, and delivers RAD51 to RPA-coated ssDNA, polymerization of more RAD51 molecules results in distancing of RPA from DNA |
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BRCA2 |
Human Breast Cancer Susceptibility Gene 2 -interacts with RAD51 and controls genome stability -mutations are thought ti be responsible for half of familial breast cancers, and also ovarian cancer and the DNA damage syndrome Fanconi Anemia -composed of BRC repeats, which each bind a Rad51 monomer -theorized that BRCA2 functions in targeting Rad51 specifically to the ssDNA region of a resected DSB |
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Stabilization of RAD51 filaments by the C-terminal region of BRCA2 |
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Proteins involved in ssBreaks |
Spo11 in Eukaryotes processing proteins: MRX (Euk) and RecBCD (Prok) assembled by: Rad52/59 (Euk) and RecBCD (Prok) |
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Pairing homologous DNA's and Strand Invasion |
RecA (Prok) Rad51, Dcm1 (Euk) |
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Introduction of DSB |
Spo11 (Euk) |
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Processing DNA Breaks to generate ss for invasion |
RecBCD (Prok) MRX=Rad50/58/60 (Euk) |
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Assembly of strand-exchange proteins |
RecBCD, RecFOR (Prok) Rad52/59 (Euk) |
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Holliday Junction recognition and branch migration |
RuvAB (Prok) |
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Resolution of Holliday Junction |
RuvC (Prok) Rad51c-XRCC3 complex, WRN, BLM (Euk) |
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Rat1 |
involved in the Torpedo Model of transcription termination in Eukaryotes where its loaded by RNA Pol and released in Poly-A sites to cleave the RNA product and terminate transcription |
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GreB |
Elongation factor that stimulates the 3' --> 5' exonuclease activity of RNA Pol in the Hydrolytic Editing form of proofreading |
