Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
20 Cards in this Set
- Front
- Back
|
RNA and DNA are neg charged, migrate to pos poles
sep based on size |
Electrophoresis
|
|
|
s blot
|
DNA
for HPV |
|
|
N blot
|
RNA
|
|
|
Dot blot
|
type of S blot
DNA not sep by size/ electrop |
|
|
DNA chip
|
holds array of ss DNA
glass slide add unknown se qdetect with mass spectro |
|
|
FISH
|
DNA
fluor molecule |
|
|
MALDI TOF
|
n.a desorbed from crystalline matrix, ionized by laser, and subj to elecrtic field
measure time it takes frags > detector |
|
|
PCR
|
denature at 94
anneal at 55 extension at 72 |
|
|
RT PCR
|
makes cDNA with RT
for RNA virus |
|
|
LCR
|
probe
no DNA synth 2 primers linked by ligase |
|
|
TMA
|
target
isothermal ampl of RNA/DNA mRNA mc transc mediated |
|
|
NASBA
|
target
isothermal method for RNA/DNA n.a. seq based |
|
|
SDA
|
probe
isothermal uses HincII 2 stage process |
|
|
CATCH
|
probe
ampl 2 target seq |
|
|
W blot
|
for ag/ab and proteins
2ndary ab used |
|
|
signal ampl
|
bDNA and HC2
|
|
|
lyses cells
|
detergent, lysis buffer
|
|
|
isolates DNA
|
protease and salt buffer
|
|
|
SDS PAGE
|
to sep proteins based on size and electrophoetic ability
gives proteins and overall negative chg |
|
|
PCR reagents
|
template, dNTPs, DNA poly, Mg ions, primers, NaCL buffer
|
