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13 Cards in this Set

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Start at the base to the ocular lens
illuminator, condenser, slide, objective, virtual image, eyepiece, eye
what does the condenser do?
collects and concentrates light from the light source
which lens projects the virtual image?
the objective
which lens focuses on the virtual image?
the ocular
How does a compound microscope work?
the objective lens produces a virtual image(meaning it is 180 degrees turned and flipped) and it is also what has the different magnifications so as the magnification increases then the virtual image becomes larger thus when the ocular lens focuses on the projected virtual image it focuses on a larger image
What does a fine adjustment knob do and where is it located
once the specimen is focused the fine adjustment knob does the fine tuning of the specimen - makes it more clear - located on the bottom part of the arm of the microscope
what does the coarse adjustment do and where is it located
while looking from the side to avoid collision you bring up the stage using the coarse adjustment and it makes the microscope able to focus on the specimen - located on the bottom part of the arm
stage adjustment knob - what does it do where is it located?
used to move the stage vertical and horizontal located beneath the stage (perpendicular)
what does oil do
decreases the amount of light that is refracted and lost
What is Brightfield Microscopy
that dark objects are visible against a bright background --> light reflected off the specimen does no enter the objective lens
Dark field Microscopy
used for specimens that are extremely light or invisible --> the only light that goes into the objective lens is that which is reflected off of the specimen
Phase Contrast Microscopy
** for living organisms - no need to fix ( stick by fire)
dont need to stain the specimen,
long waves: red, short: blue brightness = amplitude (higher = brighter)
out of phase: dim
in phase: bright
**2 light rays that are combined at the eye - one is affected by the specimen the other is unaltered
Fluorescence Microsopy
takes advantage of the ability of substances to absorb short WL and give off long WL