Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
13 Cards in this Set
- Front
- Back
|
Start at the base to the ocular lens
|
illuminator, condenser, slide, objective, virtual image, eyepiece, eye
|
|
|
what does the condenser do?
|
collects and concentrates light from the light source
|
|
|
which lens projects the virtual image?
|
the objective
|
|
|
which lens focuses on the virtual image?
|
the ocular
|
|
|
How does a compound microscope work?
|
the objective lens produces a virtual image(meaning it is 180 degrees turned and flipped) and it is also what has the different magnifications so as the magnification increases then the virtual image becomes larger thus when the ocular lens focuses on the projected virtual image it focuses on a larger image
|
|
|
What does a fine adjustment knob do and where is it located
|
once the specimen is focused the fine adjustment knob does the fine tuning of the specimen - makes it more clear - located on the bottom part of the arm of the microscope
|
|
|
what does the coarse adjustment do and where is it located
|
while looking from the side to avoid collision you bring up the stage using the coarse adjustment and it makes the microscope able to focus on the specimen - located on the bottom part of the arm
|
|
|
stage adjustment knob - what does it do where is it located?
|
used to move the stage vertical and horizontal located beneath the stage (perpendicular)
|
|
|
what does oil do
|
decreases the amount of light that is refracted and lost
|
|
|
What is Brightfield Microscopy
|
that dark objects are visible against a bright background --> light reflected off the specimen does no enter the objective lens
|
|
|
Dark field Microscopy
|
used for specimens that are extremely light or invisible --> the only light that goes into the objective lens is that which is reflected off of the specimen
|
|
|
Phase Contrast Microscopy
|
** for living organisms - no need to fix ( stick by fire)
dont need to stain the specimen, long waves: red, short: blue brightness = amplitude (higher = brighter) out of phase: dim in phase: bright **2 light rays that are combined at the eye - one is affected by the specimen the other is unaltered |
|
|
Fluorescence Microsopy
|
takes advantage of the ability of substances to absorb short WL and give off long WL
|
