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37 Cards in this Set

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Metabolism

All of the chemical reactions inside a cell

Exergonic Reactions

Reactions that are spontaneous and release energy

Endergonic Reactions

Require energy to proceed

Anabolism

Endergonic pathways converting simple molecular building blocks into more complex

Catabolism

Exergonic pathways that break down complex molecules into simpler ones

Oxidation Reaction

Reactions that remove electrons from donor molecules leaving them oxidized

Reduction Reaction

Adding electrons to acceptor molecules leaving them reduced

Redox Reactions

Pairing an oxidation reaction with a reduction reaction

Oxidation-reduction Reactions

ATP

Adenosine Triphosphate

Energy Currency

ADP

Adenosine Diphosphate

When the 3rd phosphate falls off of ATP

NAD

Nicotinamide Adenine Dinucleotide

Nicotine Adenine Dinucleotide Phosphate

NADH

Catalyst

Speeds up chemical reaction. Reusable.

Enzymes

Proteins serve as a catalyst for biochemical reactions in a cell.

Activation Energy

Energy needed to form or break chemical bonds and convert reactants to products

Substrates

The chemical reactants than enzymes bind

Catalase

Enzymes that break down hydrogen peroxide into water and oxygen

2 H2O2 -> H2O + O2

True or False:


The new lagging DNA strand is synthesized in the 5' to 3' direction only

True

Capnophiles

Require high carbon dioxide conditions to grow

What enzyme catalyzes:


H2O2 + 2H+ -> 2 H2O

Peroxidase

Label 1-6

1. DNA Polymerase III


2. Single Strand Binding Protein


3. Helicase


4. Topoisomerase/Gyrase


5. DNA Ligase


6. DNA Polymerase III

Label 1-3

1. Amino Acid


2. Start Codon


3. Anticodon

TDT; Thermal Death Time

Time during which all cells in a culture are killed

TDP; Thermal Death Point

The lowest temperature which all cells in a culture are killed in 10 minutes

DRT; Decimal Reduction Time

Time (minutes) to kill 90% of a population at a given temperature

Glycolysis

Glycolysis is the metabolic pathway that converts glucose C₆H₁₂O₆, into pyruvate, CH₃COCOO⁻ + H⁺. The free energy released in this process is used to form the high-energy molecules ATP and NADH. Glycolysis is a sequence of ten enzyme-catalyzed reactions

Steps of Calvin Benson Cycle

1. Carbon Fixation - CO2 binds to RuBP sugar with help from Rubisco


2. Reduction Phase - 3-PGA is converted into G3P


3. Carbohydrate Formation - G3P turned into carbohydrates (sugars)


4. Regeneration Phase - G3P+ATP used to make RuBP

Prion

Will not denatured in an autoclave.

List protein from most resistant

- Prions


- Endospore of Bacteria


- Mycobacterium

Phenotype

Expression of the genes

Genotype

Genes of an organism

3D structure of DNA Discovered in 1950 by

- James Watson


- Maurice Wilkins


- Rosalyn Franklin


- Cricket

Lac Inducible Operon

Catabolic (Breaks Apart)

Tryptophan Operon Repressible

Anabolic (Binds, shuts off replicant)

Process of Translation

1. Translation come together


2. tRNA pairs with start codon


3. Second codon of RNA pairs with tRNA


4. Ribosome moves the mRNA until second tRNA is in P site


5. Second amino acid joins in peptide bond, first tRNA released from E site


6. Ribosome continues along mRNA until reaching a stop codon. Polypeptide is released


7. The last tRNA is released, the ribosome comes apart, and poly peptide forms a new protein

Process of Transcription

1. RNA polymerase binds to promoter.


2. RNA is synthesized


3. Synthesis site moves along DNA


4. Transcription reaches terminator


5. RNA and RNA polymerase are released

Thioglycolate Tube Culture

Reducing medium which oxygen diffuses from the tube opening.