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25 Cards in this Set
- Front
- Back
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Conjugation |
Cell-to-cell contact of DNA transfer |
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Transduction |
Viral transfer of genes between mating bacteria |
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Transformation |
DNA acquired directly from environment |
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Plasmid |
Extrachromosomal genetic element separate from chromosome. |
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Peptidoglycan |
composedof N-acetylglucosamine, N-acetylmuramic acid, and a fewamino acids. |
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First antibodyproduced when an invading microorganism is initially encountered. |
IgM |
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Sterilization |
For media , liquids: Requires: 15 lbs pressure/in2, 121◦C (250 F), 15 minutes |
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Purpose of Stuart’s medium and Amie’s medium |
o Maintain viability of microorganisms present in a specimen o Without allowing multiplication o Contains buffers w/carbs, peptones, other nutrients excluding growth factors. |
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SPS |
Sodiumpolyanethol sulfonate, anticoagulant toprevent clotting of specimens |
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Primary plating media for CSF |
BA, CA |
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Primary plating mediafor Urine |
BA, Mac |
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Primaryplating media for Urethral specimen |
BA,CA, Thayer-Martin-forNeisseria gonorrhoeae |
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Primaryplating media for respiratory specimens: sputum, bronchial etc. |
BA, CA, Mac |
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Primary plating media for stool specimens |
BA, Mac, HE, Campy |
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Gram negative diplococci |
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PMNS and Gram (-) diplococci (N. gonorrhoeae) |
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WBC's and Gram (+) bacilli. (C. perfringens) |
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PMNS and Gram (+) cocci (note tapered ends) (S. pneumoniae) |
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PMNS and Gram negative bacilli (small gram negative coccobacilli) |
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PMNS and Gram (+) cocci in chains |
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PMNS and Gram negative bacilli |
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Steps for Acid-Fast Stains |
- Carbolfuchsin (enters mycolic acid wax-containing cell) - Decolorize (3% HCL in 95% ethanol) - Methylene blue (counterstain) |
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Most commonly used acid fast stain |
Kinyoun & Ziehl-Nielson |
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Steps in Auramine - Rhodamine stain |
- A&R (fluorescent stain) - Acid Alcohol - Potassium Permanganate -orange fluorescence = positive result (used for mycobacteria) |
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Steps in Calcofluor white stain |
-Fluorescent stain - Bluish white fluorescence = positive result - (used for yeast and fungi) |
