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9 Cards in this Set

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Classification by shape

Bacillus rod shapes


Cocci spherical


Spirillum corkscrew

B


C


S

Gram stain classification

Gram positive or negative bacteria


Due to cell way ( polysaccharides and polypeptides-peptidoglycanPrevents osmotic lysis


and polypeptides-peptidoglycan


Prevents osmotic lysis

Gran negative

More complex cell walls


Peptidoglycan is supplemented by lipoplysaccharides


Dont retain crystal violet


Appear red


Not affected by antibacterial enzyme lysozyme e.g.penicillin

Gram positive

Lack lipopolysaccharide


Retain crystal violet


Appear purple


More susceptible to antibiotics

Conditions necessary for growth

Bacteria can divide once ever 20 minutes in optimum conditions


Conditions vary between bacteria

Micro organisms requirements (nutrients)

Nutrients - supplied by agar in labs


Contain carbon( glucose) nitrogen in organic and non oganic, vitamind and mineral salts

Micro organism requirements ( temperature)

Growth is normally regulated by enzymes


25-45°c is favoured by most bacteria


Optimum for mammal pathogens is 37°c

Microorganism requirements ( pH)

Most prefer slightly alkaline conditions (7.4)


Fungi grow better in slightly acidic


Micro organism requirements (oxygen)

Oxygen is required for metabolic processes in obligate aerobes and facultative anaerobes


Obligate anaerobes cant grow in its presence e.g. clostridium causes gangrene in wounds