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38 Cards in this Set
- Front
- Back
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Trypticase soy agar
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General purpose
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Brain heart infusion agar
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Highly nutritious general purpose
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Eosin methylene blue
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Differential and selective, eosin and methylene dyes are ph indicators. Select for enterobacteriaceae. Lac+ (high acid and low acid) and lac -
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Mannitol salt agar
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Selective and differential, 7.5% salt. Selects for halophiles, inhibits most bacteria. Staphylococcus epidermidis is positive, streptococcus lactis is negative
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Sabouraud dextrose agar
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Selective and differential. Medium used to grow fungi
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Blood agar
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Enrichment agar
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Most commonly used buffer in micro
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Phosphate buffer
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3% koh test
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Used to determine whether a bacteria is gram negative or gram positive
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What can cause a failure of transfer?
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Loop or needle has not cooled
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Button
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Obligate anaerobes
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Pellicle
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Obligate aerobes
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Uniform turbidity
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Facultative anaerobes
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Autoclave
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121/15 psi/15-20 min
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Brownian motion
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Not true motility
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Peritrichous
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Escherichia coli
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Monotrichous
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Pseudomonas aeruginosa
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Lophotrichous
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Pseudomonas marginalis
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Amphitrichous
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Spirillum volutans
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Axial filaments
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Borellia burgdorferi
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Microaerophiles
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Catalase test
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Catalase breaks hydrogen peroxide down to water and oxygen
Positive - staphylococcus epidermidis Negative - streptococcus lactis |
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What additional enzymes must organisms possess if they are able to ferment sucrose and lactose ?
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Sucrase and beta galactosidase
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What is happening when the butt turns black in an h2s experiment?
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Sulfur ions combine with iron ions to form iron sulfide
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Hydrogen Sulfide Test
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Black butt + proteus mirabilis
No black - E.coli Yellow butt only -only glu fermented Yellow butt and slant - glu and suc/lac fermented Cracks - gas produced during fermentation |
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Alkaline without gas, A. Faecalis
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B. Subtilis, all acidic but lactose
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E.coli ferments lac and glu and produces gas. Not with suc!
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Emb
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Selective ingredients are eosin and methylene blue, inhibits gram + and fastidious gram -, selects for enterobacteriaceae, differential ingredients is dyes and lactose
Lac + high fermenter e.coli Lac + low fermenter e. Aerogenes Lac - salmonella |
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Blood agar
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Enrichment medium, inhibits none, selects for growth of fastidious organisms, differential ingredient blood
Beta hemolysis - staphylococcus. Aureus Alpha hemolysis - streptococcus pneumoniae |
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Msa
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Selective ingredient 7.5 % nacl, inhibits most bacteria, selects for salt tolerant skin microbes like staphylococcus aureus, ph indicator is phenol red
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Sabouraud dextrose agar
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Low ph 5.6, inhibits many bacteria, selects for fungi
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H antigens
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Flagellar antigens in seri typing procedures
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Motility
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Ability of organism to move on its own, increasing it's chance for survival
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Phase variation
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Type of flagellar proteins can be altered
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Hanging drop slide
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Depression slide used with petroleum jelly to observe motility
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Taxis
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Directed movement of bacteria toward or away from a stimulus
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Difference between gram - and gram + flagellar structure
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Gram- have 4 basal rings, gram + have 2
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Advantage of solid media
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Permits isolation of cultures
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