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35 Cards in this Set
- Front
- Back
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Psychrophiles |
less than 20, not pathogen |
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Psychrotrophs |
0-30, pathogen |
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Mesophiles |
15-45, pathogen |
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Thermophiles |
40-70, not pathogen |
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Hyperthermophiles |
65-110, not pathogen |
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Acidophile |
less than 5 |
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Neutrophile |
5-8 |
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alkaphile |
8 and up |
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Thioglycollate medium contains: |
Sodium thioglycollate, L-cysteine, resazurin... aerotolerance |
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Anaerobic Jar indicator colors |
white=non-oxygen... blue=oxygen |
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OF test purpose |
shows whether organism uses fermentation or oxidation |
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OF Media components |
bromethmol blue, carbohydrate source |
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Phenol Red Broth contains |
phenol red, durham tube, add carb of choice |
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purpose of phenol red broth test |
which carbohydrates a bacteria can ferment |
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Nitrate reduction test purpose |
Determines whether an organism can use nitrate to make ammonia (ammonification) or nitrogen (denitrification) |
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Nitrate broth contains... |
potassium nitrate, durham tube |
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purpose of decarboxylation test |
determine presence or absence of specific decarboxylase |
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Decarboxylase medium contains |
proteins, carbohydrates, pyridoxal phosphate, bromocresol purple |
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Decarboxylase coenzyme |
pyridoxal phosphate |
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End product of decarboxylase |
polyamines |
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condition for decarboxylase to work |
acidic and anaerobic to make acidic |
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MR VP broth |
peptone, glucose, phosphate buffer |
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MR reagent |
methyl red |
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VP reagent |
a-napthol and KOH |
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Citrate test purpose |
to determine which organisms can grow in citrate (C-source) and ammonium phosphate (P-source) |
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Ammonium phosphate broken down into what? |
citrase test.... alkaline products |
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Indole test purpose |
to determine which organisms have specific deaminase for tryptophan |
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reagent for indole reaction |
kovac's reagent |
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indole reacts with what component of kovac's reagent |
p-DMAB |
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p-DMAB reactions with indole to make what? |
Red Rosindole Dye complex |
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Indole test broth contains |
tryptophan (no pH indicator) |
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IMViC tests used for what |
differentiate between enteric bacteria and/or coliforms |
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Purpose of urea test |
to determine if organism has urease to break down urea to ammonia and co2 |
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urea broth components |
yeast extract, urea, potassium phosphate buffer, phenol red pH indicator |
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a-amylase breaks what bonds |
glycosidic bonds |
