Micro Lab Midterm

Front 1

List the first 4 cardinal rules of microbio safety:

Back 1

1. Do not mouth-pipette
2. Manipulate infectious fluids carefully
3. Restrict usage of needles and syringes
4. Use protective lab clothing and safety glasses

Front 2

List the last 3 cardinal rules of microbio safety:

Back 2

5. Wash hands after any overt contact and when leaving the lab
6. Decontaminate work surfaces
7. Don't eat, drink or put on make-up

Front 3

What are the two general forms of media:

Back 3

1. Liquid (broth)
2. semi-solid (agar)

Front 4

Two forms of general purpose media:

Back 4

1. Nutrient broth/agar (NB/NA)
2. Tryptic Soy broth/agar (TSB/TSA)

Front 5

What does an autoclave NOT kill?

Back 5

Prions

Front 6

List the four types of sterilization used in the microlab:

Back 6

1. Autoclave
2. Gamma radiation
3. Bunsen burner
4. Electric loop/needle sterilizer

Front 7

The ________ _________ method is the most important isolation tool.

Back 7

Streak Plate

Front 8

Streaking results in _________ __________ to a single bacterium. The resulting colony will be _______ of one another.

Back 8

Limiting dilution
Clones

Front 9

Agar deep stabs are used to test for ______________.

Back 9

Aerotolerance

Front 10

Fluid thioglycollate medium tests for ___________ microbes.

Back 10

Anarobic

Front 11

Sodium thioglycolate binds ________, thus preventing its use by microbes.

Back 11

Oxygen

Front 12

When using oil immersion, only the _____X objective should be used.

Back 12

100

Front 13

The ______ stain is the most important stain in microbiology.

Back 13

Gram

Front 14

Gram ____ bacteria appear purple while Gram ____ bacteria appear pink.

Back 14

Positive = Purple
Negative = Pink

Front 15

Acid fast stains are primarily used to ID ____________ species.

Back 15

Mycobacterial

Front 16

The ______ stain is a differential stain based upon ______ _____ composition

Back 16

Gram
Cell wall

Front 17

Crystal violet is a _______ dye.

Back 17

Basic

Front 18

Gram's Iodine is a fixing agent, AKA a ___________.

Back 18

Mordant

Front 19

Gram's Iodine covalently links crystal violet to a Gram _______ bacteria's cell wall.

Back 19

Positive

Front 20

_________ alcohol is used to rinse away unbound dye and to __________ Gram negative cells.

Back 20

Acetone
Decolorize

Front 21

A counter stain, _________ is used to stain Gram ________ bacteria pink.

Back 21

Safranin
Negative

Front 22

Mycobacteria have _________ acid in their cell walls, making the ______ _______ staining technique very useful.

Back 22

Mycolic Acid
Acid Fast

Front 23

_______ ________ dye binds to mycolic acid and is acid-alcohol resistant.

Back 23

Carbol Fuchion

Front 24

___________ _________ is the counter stain in acid fast staining procedure.

Back 24

Methylene Blue

Front 25

________ Alcohol is te decolorizer in acid fast stain procedure.

Back 25

Acid

Front 26

List the primary, mordant, decolorizer and counter stain for Gram Staining:

Back 26

1. Crystal Violet
2. Gram's Iodine
3. Acetone Alcohol
4. Safranin

Front 27

List the primary, mordant, decolorizer and counter stain for Acid Fast Staining:

Back 27

1. Carbol Fuchion
2. Heat
3. Acid Alcohol
4. Methylene Blue

Front 28

Mannitol Salt Agar

Back 28

High salt concentration
Mannitol fermentation

Front 29

Phenol Ethanol Agar

Back 29

Tolerance to Phenol Ethanol

Front 30

Desoxycholate Agar

Back 30

Tolerance to desoxycholate
Lactose fermentation

Front 31

Eosin Methylene Blue Agar

Back 31

Tolerance to eosin and MB dye
Lactose and sucrose fermenta.
Acid production

Front 32

MacConkeys Agar

Back 32

Tolerance to crystal violet and bile
Lactose fermentation
Acid production

Front 33

Hektoen Agar

Back 33

Tolerance to bile
Tolerance to acid fuchion and bromthymol blue dyes.
Lactose fermentation
Acid production
Reduction of sulfur source (to hydrogen sulfide)

Front 34

Xylose Lysine Desoxycholate

Back 34

Tolerance to desoxycholate
Xylose fermatation and/or acid.
Reduction of sulfur source
Metabolism of lysine amino acid

Front 35

Immersion oil constrains __________ (light-scatter)

Back 35

Defraction

Front 36

Which bacteria appeared yellow on the NA plate and the Mannitol Salt Agar?

Back 36

Staphylococcus aureus

Front 37

What two bateria appeared to be fermenting acid from mannitol on the Mannitol Salt Agar?

Back 37

Staphylococcus aureus and
Enterobacter aerogenes

Front 38

Which bacteria was inhibited by the high salt concentration in the Mannitol Salt Agar?

Back 38

Escherichia coli

Front 39

Which bateria was inhibited by the phenol ethanol on the Phenol Ethanol plate?

Back 39

Enterococcus faecalis

Front 40

What does an inhibition from Phenol Ethanol tell you about the bateria that is inhibited?

Back 40

It is probably a Gram neg. bacteria (like Enterococcus faecalis)

Front 41

Describe the Staphylococcus aureus that was sreaked onto the deoxycholate agar:

Back 41

Staphylococcus aureus: Inhibited by desoxy. Probably Gram pos.

Front 42

Which bacteria was Pink/red indicating that it was inhibited by desoxycholate and fermented lactose to acid?

Back 42

Escherichia coli

Front 43

Which bacteria was not inhibited by desoxycholate, but did not ferment?

Back 43

Salmonella typhimurium

Front 44

Which three bacteria were streaked onto the darker red desoxycholate agar?

Back 44

Escherichia coli
Staphylococcus aureus
Salmonella typhimurium

Front 45

Descirbe staph. aureus's growth on the eosin methylene blue agar:

Back 45

Colorless, no pink, purple or metallic sheen

Front 46

Describe E. coli's growth on the EMB agar:

Back 46

Growth was pink and mucoid

Front 47

Describe Proteus hauseri's growth on the EMB agar:

Back 47

Growth was dark (purple to black with or without green sheen).

Front 48

Which bacteria was inhibited on the MacConkey agar and what was it inhibited by?

Back 48

Staphylococcus aureus,
Inhibited by crystal violet and/or bile.

Front 49

What two bacteria were not inhibited on the MacConkey agar and produced acid thru fermentation?

Back 49

Escherichia coli and
Enterobacter aerogenes

Front 50

Which organism was not inhibited on the MacConkey agar but did not ferment (shown thru colorlessness)?

Back 50

Proteus hauseri

Front 51

What color of bacteria on the MacConkey agar indicates the bacteria is fermenting acid?

Back 51

Red/pink growth

Front 52

On the Hektoen agar, E. coli was:

Back 52

A blue-green growth without black ppt.

Front 53

On the Hektoen agar, S. typhi was:

Back 53

Blue-green with a black ppt

Front 54

What two bacteria did not grow on the Hektoen agar indicating an inhibition to bile and/or acid fuchion or bromthymol blue dyes?

Back 54

Staphylococcus aureus and
Shigella flexneri

Front 55

What does an inhibition from Phenol Ethanol tell you about the bateria that is inhibited?

Back 55

It is probably a Gram neg. bacteria (like Enterococcus faecalis)

Front 56

Describe the Staphylococcus aureus that was sreaked onto the deoxycholate agar:

Back 56

Staphylococcus aureus: Inhibited by desoxy. Probably Gram pos.

Front 57

Which bacteria was found to be not inhibited by desoxycholate and fermented lactose to acid?

Back 57

Escherichia coli

Front 58

Which bacteria was not inhibited by desoxycholate, but did not ferment?

Back 58

Salmonella typhimurium

Front 59

Which three bacteria were streaked onto the darker red desoxycholate agar?

Back 59

Escherichia coli
Staphylococcus aureus
Salmonella typhimurium

Front 60

What does an inhibition from Phenol Ethanol tell you about the bateria that is inhibited?

Back 60

It is probably a Gram neg. bacteria (like Enterococcus faecalis)

Front 61

Describe the Staphylococcus aureus that was sreaked onto the deoxycholate agar:

Back 61

Staphylococcus aureus: Inhibited by desoxy. Probably Gram pos.

Front 62

Which bacteria was found to be not inhibited by desoxycholate and fermented lactose to acid?

Back 62

Escherichia coli

Front 63

Which bacteria was not inhibited by desoxycholate, but did not ferment?

Back 63

Salmonella typhimurium

Front 64

Which three bacteria were streaked onto the darker red desoxycholate agar?

Back 64

Escherichia coli
Staphylococcus aureus
Salmonella typhimurium

Front 65

Which bacteria was inhibited on the XLD agar?

Back 65

Staphylococcus aureus

Front 66

Which bacteria appeared red with a black center on the XLD agar and what does this indicate?

Back 66

Salmonella typhimurium,
Indicates that it is reducing sulfer to hydrogen sulfide

Front 67

Which bacteria appeared yellow on the XLD agar and what does this indicate?

Back 67

Enterobacter aerogenes,
Produces acid from xylose fermentation.

Front 68

Which bacteria was inhibited on the XLD agar?

Back 68

Staphylococcus aureus

Front 69

Which bacteria appeared red with a black center on the XLD agar and what does this indicate?

Back 69

Salmonella typhimurium,
Indicates that it is reducing sulfer to hydrogen sulfide

Front 70

Which bacteria appeared yellow on the XLD agar and what does this indicate?

Back 70

Enterobacter aerogenes,
Produces acid from xylose fermentation.

Front 71

Which bacteria was inhibited on the XLD agar?

Back 71

Staphylococcus aureus

Front 72

Which bacteria appeared red with a black center on the XLD agar and what does this indicate?

Back 72

Salmonella typhimurium,
Indicates that it is reducing sulfer to hydrogen sulfide

Front 73

Which bacteria appeared yellow on the XLD agar and what does this indicate?

Back 73

Enterobacter aerogenes,
Produces acid from xylose fermentation.