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73 Cards in this Set

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List the first 4 cardinal rules of microbio safety:
1. Do not mouth-pipette
2. Manipulate infectious fluids carefully
3. Restrict usage of needles and syringes
4. Use protective lab clothing and safety glasses
List the last 3 cardinal rules of microbio safety:
5. Wash hands after any overt contact and when leaving the lab
6. Decontaminate work surfaces
7. Don't eat, drink or put on make-up
What are the two general forms of media:
1. Liquid (broth)
2. semi-solid (agar)
Two forms of general purpose media:
1. Nutrient broth/agar (NB/NA)
2. Tryptic Soy broth/agar (TSB/TSA)
What does an autoclave NOT kill?
Prions
List the four types of sterilization used in the microlab:
1. Autoclave
2. Gamma radiation
3. Bunsen burner
4. Electric loop/needle sterilizer
The ________ _________ method is the most important isolation tool.
Streak Plate
Streaking results in _________ __________ to a single bacterium. The resulting colony will be _______ of one another.
Limiting dilution
Clones
Agar deep stabs are used to test for ______________.
Aerotolerance
Fluid thioglycollate medium tests for ___________ microbes.
Anarobic
Sodium thioglycolate binds ________, thus preventing its use by microbes.
Oxygen
When using oil immersion, only the _____X objective should be used.
100
The ______ stain is the most important stain in microbiology.
Gram
Gram ____ bacteria appear purple while Gram ____ bacteria appear pink.
Positive = Purple
Negative = Pink
Acid fast stains are primarily used to ID ____________ species.
Mycobacterial
The ______ stain is a differential stain based upon ______ _____ composition
Gram
Cell wall
Crystal violet is a _______ dye.
Basic
Gram's Iodine is a fixing agent, AKA a ___________.
Mordant
Gram's Iodine covalently links crystal violet to a Gram _______ bacteria's cell wall.
Positive
_________ alcohol is used to rinse away unbound dye and to __________ Gram negative cells.
Acetone
Decolorize
A counter stain, _________ is used to stain Gram ________ bacteria pink.
Safranin
Negative
Mycobacteria have _________ acid in their cell walls, making the ______ _______ staining technique very useful.
Mycolic Acid
Acid Fast
_______ ________ dye binds to mycolic acid and is acid-alcohol resistant.
Carbol Fuchion
___________ _________ is the counter stain in acid fast staining procedure.
Methylene Blue
________ Alcohol is te decolorizer in acid fast stain procedure.
Acid
List the primary, mordant, decolorizer and counter stain for Gram Staining:
1. Crystal Violet
2. Gram's Iodine
3. Acetone Alcohol
4. Safranin
List the primary, mordant, decolorizer and counter stain for Acid Fast Staining:
1. Carbol Fuchion
2. Heat
3. Acid Alcohol
4. Methylene Blue
Mannitol Salt Agar
High salt concentration
Mannitol fermentation
Phenol Ethanol Agar
Tolerance to Phenol Ethanol
Desoxycholate Agar
Tolerance to desoxycholate
Lactose fermentation
Eosin Methylene Blue Agar
Tolerance to eosin and MB dye
Lactose and sucrose fermenta.
Acid production
MacConkeys Agar
Tolerance to crystal violet and bile
Lactose fermentation
Acid production
Hektoen Agar
Tolerance to bile
Tolerance to acid fuchion and bromthymol blue dyes.
Lactose fermentation
Acid production
Reduction of sulfur source (to hydrogen sulfide)
Xylose Lysine Desoxycholate
Tolerance to desoxycholate
Xylose fermatation and/or acid.
Reduction of sulfur source
Metabolism of lysine amino acid
Immersion oil constrains __________ (light-scatter)
Defraction
Which bacteria appeared yellow on the NA plate and the Mannitol Salt Agar?
Staphylococcus aureus
What two bateria appeared to be fermenting acid from mannitol on the Mannitol Salt Agar?
Staphylococcus aureus and
Enterobacter aerogenes
Which bacteria was inhibited by the high salt concentration in the Mannitol Salt Agar?
Escherichia coli
Which bateria was inhibited by the phenol ethanol on the Phenol Ethanol plate?
Enterococcus faecalis
What does an inhibition from Phenol Ethanol tell you about the bateria that is inhibited?
It is probably a Gram neg. bacteria (like Enterococcus faecalis)
Describe the Staphylococcus aureus that was sreaked onto the deoxycholate agar:
Staphylococcus aureus: Inhibited by desoxy. Probably Gram pos.
Which bacteria was Pink/red indicating that it was inhibited by desoxycholate and fermented lactose to acid?
Escherichia coli
Which bacteria was not inhibited by desoxycholate, but did not ferment?
Salmonella typhimurium
Which three bacteria were streaked onto the darker red desoxycholate agar?
Escherichia coli
Staphylococcus aureus
Salmonella typhimurium
Descirbe staph. aureus's growth on the eosin methylene blue agar:
Colorless, no pink, purple or metallic sheen
Describe E. coli's growth on the EMB agar:
Growth was pink and mucoid
Describe Proteus hauseri's growth on the EMB agar:
Growth was dark (purple to black with or without green sheen).
Which bacteria was inhibited on the MacConkey agar and what was it inhibited by?
Staphylococcus aureus,
Inhibited by crystal violet and/or bile.
What two bacteria were not inhibited on the MacConkey agar and produced acid thru fermentation?
Escherichia coli and
Enterobacter aerogenes
Which organism was not inhibited on the MacConkey agar but did not ferment (shown thru colorlessness)?
Proteus hauseri
What color of bacteria on the MacConkey agar indicates the bacteria is fermenting acid?
Red/pink growth
On the Hektoen agar, E. coli was:
A blue-green growth without black ppt.
On the Hektoen agar, S. typhi was:
Blue-green with a black ppt
What two bacteria did not grow on the Hektoen agar indicating an inhibition to bile and/or acid fuchion or bromthymol blue dyes?
Staphylococcus aureus and
Shigella flexneri
What does an inhibition from Phenol Ethanol tell you about the bateria that is inhibited?
It is probably a Gram neg. bacteria (like Enterococcus faecalis)
Describe the Staphylococcus aureus that was sreaked onto the deoxycholate agar:
Staphylococcus aureus: Inhibited by desoxy. Probably Gram pos.
Which bacteria was found to be not inhibited by desoxycholate and fermented lactose to acid?
Escherichia coli
Which bacteria was not inhibited by desoxycholate, but did not ferment?
Salmonella typhimurium
Which three bacteria were streaked onto the darker red desoxycholate agar?
Escherichia coli
Staphylococcus aureus
Salmonella typhimurium
What does an inhibition from Phenol Ethanol tell you about the bateria that is inhibited?
It is probably a Gram neg. bacteria (like Enterococcus faecalis)
Describe the Staphylococcus aureus that was sreaked onto the deoxycholate agar:
Staphylococcus aureus: Inhibited by desoxy. Probably Gram pos.
Which bacteria was found to be not inhibited by desoxycholate and fermented lactose to acid?
Escherichia coli
Which bacteria was not inhibited by desoxycholate, but did not ferment?
Salmonella typhimurium
Which three bacteria were streaked onto the darker red desoxycholate agar?
Escherichia coli
Staphylococcus aureus
Salmonella typhimurium
Which bacteria was inhibited on the XLD agar?
Staphylococcus aureus
Which bacteria appeared red with a black center on the XLD agar and what does this indicate?
Salmonella typhimurium,
Indicates that it is reducing sulfer to hydrogen sulfide
Which bacteria appeared yellow on the XLD agar and what does this indicate?
Enterobacter aerogenes,
Produces acid from xylose fermentation.
Which bacteria was inhibited on the XLD agar?
Staphylococcus aureus
Which bacteria appeared red with a black center on the XLD agar and what does this indicate?
Salmonella typhimurium,
Indicates that it is reducing sulfer to hydrogen sulfide
Which bacteria appeared yellow on the XLD agar and what does this indicate?
Enterobacter aerogenes,
Produces acid from xylose fermentation.
Which bacteria was inhibited on the XLD agar?
Staphylococcus aureus
Which bacteria appeared red with a black center on the XLD agar and what does this indicate?
Salmonella typhimurium,
Indicates that it is reducing sulfer to hydrogen sulfide
Which bacteria appeared yellow on the XLD agar and what does this indicate?
Enterobacter aerogenes,
Produces acid from xylose fermentation.