Micro Lab

Front 1

TSI

Some bacteria break down the ______. ______

________> _______ +________+_________

Back 1

Amino acid Cysteine, cysteine,

Cysteine desulfurose, pyruvic acid, NH3,

H2S

Front 2

There is ____ in the media. The ___________( ) will

bind to _______ & _______ it ( must like ____ _____

______) only _____ causes ________ to turn into a ________ ___________ precipitate

Back 2

Iron, hydrogen sulfide, (H2S), iron, rust, 02,

Rust, iron, H2S, iron, black, iron sulfide

Front 3

Result or TSI

_______________ _____________

Back 3

+H2S= black fuzzy caterpillar

-H2S=no black fuzzy caterpillar

Front 4

Urea

Some bacteria break down urea. Urea ______>

________ +_______. There is __________ already in the media. Phenol Red turns ______ in alkaline

conditions.

Back 4

urease NH3 + CO2. Phenol Red, Phenol Red turns _________ in alkaline conditions.

Front 5

Urea Results:

______________ _______________

Back 5

Urease += Pink

Urease-=Salmon

Front 6

Citrate

Tests to see if bacteria can use ______ (instead of

_______) as a source of ______. When bacteria
use Citrate, it converts the ____________ into

______. NH3 raise the ph causing Bromthyma Blue to change color from _____ to ___________.

Back 6

Citrate, glucose, carbon, ammonium salt,

ammonia, ___________ to __________

Front 7

Citrate result:

______________ _________________

Back 7

Citrate + = blue

Citrate - = Green

Front 8

VP (2,3 butanedoil test

Some MR- organisms make _________________

instead of ________. During fermentation we can't directly detect ______________. It convert it back to _________& directly test for it. Add ______________of ____________. Into the vortex every _______ for up to ________.

Back 8

2,3 butanediol , mixed acids, 2,3 butanediol,

acetoin, 15 drops Barritt's A & B. 5 min ,30 min

Front 9

VP result:

_______________ __________________

Back 9

VP + = Brick red

VP - = orange

Front 10

Oxidase

_________organisms use _________________ as __________ in the ETC. This test to see if

bacteria use __________, specifically, as opposed to some other oxidase. Use sterile swabs to pick up some of each bacteria.

Back 10

Aerobic, proteins/enzyme , e- carrier ,

Cytochrome oxidase

Front 11

Oxidase test: Add ___ drop of ____________ to each swabs.

Back 11

one, oxidase reagent

Front 12

Oxidase results and color:

___________ ____________ ______________

Back 12

color Blue is positive

Oxidase + = immediate blue

Oxidase - = no blue

Front 13

Catalase This test is done after the Oxidase.

Back 13

This test is done after the Oxidase

Front 14

Catalase

Test to see if bacteria use Catalase as an _______ to neutralize _______ _______ of ____________.

_________ _________> _________ + ___________. Then add a few drops of Hydrogen peroxide to each bacteria on the plate & observe.

Back 14

enzyme, toxic forms of oxygen,

2H2O2 → Catalase →2H2O + O2

Front 15

Catalase result:

_______________ ______________

Back 15

Catalase + = bubbles

Catalase - = no bubbles

Front 16

Amylase

Tests to see if bacteria can break down ______ into __________ __________(____________).

_______>___________> ______ ________ (_______). Add

_____________ to plate & swirl and dump excess down the drain. If there a ______ zone around the colony. The bacteria _______ starch.

Back 16

starch , simple sugars (hydrolyze), Gram's iodine,

clear, "ate"

Front 17

Amylase results:

___________ _____________

Back 17

Amylase + = clear zone

Amylase - = no clear zone