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15 Cards in this Set
- Front
- Back
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What are the differnt stains?
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1) Gram stain
2) Acid fast stain 3) Endospore |
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What is the purpose of stains?
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To increase the contrast of bactyerial cellsand their environment
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Wat is differential staining?
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It is to distinguish between different types of bacteria because of physical and chemical differences
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What is the gram stain
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Differential stain :
1) Crystal violete (30 sec) stains the gram+ cell 2) Iodine(1 min) interacts with the crystal violete 3) 95%ethanol or 70%ethanol 30% acetone (G+ shrink murien to keep crystal violete) &(G- extract lipids from cell wall allowing porosity) 4) Safarin(1min) = counter stain makes colorless cells pink (G-) |
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What is the best age for gram + cells to be stained?
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14 Hrs
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What is acid fast stain?
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Differential stain: i.e: Micobacteria (stain for Micolic acid in cell wall)
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What is the components of the acid fast stain?
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1) Carbolfuchin (primary stain)= lipid soluble stain micolic acid
2) Acid alcohol (decolorizes non-acid fast cells) 3) Methelene blue(secodary stain stain non acid fast cells blue) |
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What is the endospore stain for?
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Differencial stain: locating the presence and position of endospores.ie: Bacillus & clostridium
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Waht is an endospore?
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1)A dormand for of the bacterium that helps it withstand the environment, and when favored it germinates to vegitative cells
2) Endospores have a hard keratin cover |
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What is the components of the endospore?
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1) Malachite green (primary) stain endospore with heat
2) water decolorize 3) saffranin counterstain the vegetative cells |
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What is the two types of acid fast stains?
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1) Ziehl-neelsen stain
(Methylene blue)- Non acid 2) Kinyoun Acid-fast stain (Brilliant green- Non acid |
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What is a nother way other than motility agar that one can see if a cell is motile?
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Phase contrast
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What is a good way to look at a capsule through the microscape?
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Through phase contrast
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What is the differnt consistencies of agar media?
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Liquid, solid(1.5 % agar) & semi-solid (.4% to 1% agar)
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What is the different ways to paur agar solids?
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–Plates
–Deeps to pour plates –Deeps for anaerobic cultures –slants |
