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29 Cards in this Set
- Front
- Back
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diffuse growth radiating outward from stab line (interpretation and bacteria)
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motile; Escherichia coli
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growth only along stabline (interpretation and bacteria)
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non-motile; Klebsiella pneumoniae
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growth radiating from the streak line usually covering the entire surface of the plate (interpretation and bacteria)
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swarming motility; Proteus mirabilis
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Protues mirabilis exhibits what type of flagella arrangement?
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peritrichious
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SIM tube contains __% agar
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0.35
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TSA plate contains __% agar
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1.5
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this bacterium contains a high concentration of mycolic acid in the cell wall
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mycobacterium
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mycobacterium require long exposure to what kind of dye
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carbolfuchsin
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what two bacterium were used for acid fast stain
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Mycobacterium smegmatis and Staphylococcus aureus
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Kinyoun acid fast stain for M. smegmatis and S. aureus (interpretation and appearance)
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M. smegmatis: red rods (acid-fast)
S. aureus: blue rods (non--acid fast) |
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Schaeffer-Fulton procedure is used for what kind of stain
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endospore (quick-spore stain)
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Schaeffer-Fulton procedure uses what two types of dye, and what is the interpretation
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malachite green (spores) and safranin (vegetative cells)
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what two organisms were used for the streak plate method
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Serratia marcescens? and E. coli
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For streak plate, how long and what temp is incubation
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48 hours at 25C
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for pour plate method, S. marcescens produces what color pigment at 25C
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red
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TSA
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tryptc soy agar
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what procedure involves sticking a hockey stick in 95% alcohol
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spread plate
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complex polysaccharide isolated from seaweed that is solidifying agent used in media preparation
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agar
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agar usually added in concentrations of __%
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1.5
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medium that causes bacteria to take on an appearance that distinguishes them from other bacteria
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differential medium
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differentiates Staphylococcus species based on ability to ferment mannitol
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mannitol salt agar
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in mannitol salt agar, what indicates fermentation of mannitol that results in a lowering of pH of medium
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color change from pink to yellow
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differentiatesbetween gram-negative rods based on ability to ferment lactose
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eosin methylene blue agar
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in EMB what indicates lactose fermenters other than E. coli
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dark colored colonies
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in EMB what indicates E. coli
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green metallic sheen
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allows certain bacteria to grow but is inhibitory to others
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selective medium
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mannitol salt agar is inhibitory to what due to what
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gram-negative rods, high salt concentration
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EMB is inhibitory to what due to what
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gram positive organisms; eosin and methylene blue
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autoclave involves heating media at ___C for __ minutes at ___ psi
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121C, 15-20 minutes, 15psi
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