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10 Cards in this Set
- Front
- Back
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what is recombinant dna? |
artificial dna |
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what is a restriction enzyme? |
cuts dna in palindromic sequence and selects specific genes |
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how do restriction enzymes recognize dna? |
by methyl group |
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what does the PCR do? |
amplifies dna |
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what are the 3 steps of the invivo PCR? |
1. dna helicase 2. primer 3. DNA polymerase |
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what are the 3 steps of invitro PCR? |
1. denaturation 2. dna primers 3. taq polymerase |
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what is denaturation? |
95 degree heat breaks double stranded dna |
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what is annealing? |
at 60 degrees primers can add on |
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what is elongation? |
at 72 degrees taq polymerase elongates to make a daughter strand |
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what is taq polymerase? |
adds dna nucleotides |
