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10 Cards in this Set
- Front
- Back
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microbiology
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study of microorganisms
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Pasteur
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• Established microbial role
in fermentations • Refuted the theory of spontaneous generation • Helped establish the germ theory of disease • Developed vaccination for cholera (poultry), anthrax, rabies |
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Leeuwenhoek
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(1633-1723)
• developed first microscope |
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Robert Koch
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(1843-1910)
• Helped establish the germ theory of disease (Koch’s postulates, 1878). • Made many technological breakthroughs in lab methods. |
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general medium
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Non-selective, all species grow.
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differential medium
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Certain species exhibit a particular characteristic.
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Koch's Postulates
(germ theory of disease) |
1. The microorganism must be found in abundance in all organisms suffering from the disease.
2. The microorganism must be isolated from a diseased organism and grown in pure culture. 3. The cultured microorganism should cause disease when introduced into a healthy organism. 4. The microorganism must be reisolated from the inoculated, diseased experimental host and identified as being identical to the original specific causative agent. |
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isolation
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1. loop dilution
2. streak plate |
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eukaryotes
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• membrane-bound nucleus contains chromosomal DNA
• several chromosomes • 80s ribosomes in cytoplasm, 70s ribosomes in organelles • membrane-bound organelles present • cytoskeleton (microtubules and microfilaments) present • cytoplasmic streaming occurs • larger cell size |
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prokaryotes
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• no true nucleus (no membrane surrounds DNA)
• single, circular chromosome • 70s ribosomes • no membrane-bound organelles are present • no microtubules • no cytoplasmic streaming occurs • small cell size |
