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237 Cards in this Set

  • Front
  • Back
Staph aureus color, Staph epidermidis color
yellow, white
S aureus virulence factors
Protein A, teichoic acid (adherence), Capsule (inhibits phagocytosis)
S aureus penicillin resistance?
yes
S aureus preferentially colonizes the ?
nares
For culturing staph, what is a nonselective media, and a selective media?
sheep blood and chocolate agar are none selective, mannitol salt agar is selective
Strep mode of metabolism?
Oxygen tolerant anaerobic
Strep A, hemolysis, bacitracin, pyrase,
Beta, sensitive, positive (red)
Pneumonia
lecture
Most common causitive agent
Bacterial
pathophysiology, 4 steps
1. Infection of lung parenchyma by organism
2. Neutrophils migrate: pulm capiliaries  alveolar spaces
3. Neutrophils phagocytose & kill injested microbe
4. Inflammation  neutrophilic exudate in alveolar spaces – prevents O2 exchange (SOB)
Most common cause of pneumonia
S. pneumoniae
X-ray finding for inhalation of anthrax
wide mediastinum
S. pneumoniae penicillin resistence?
yes
Strain associated with COPD and alcoholism
Hemophilus influenzae (penicillin resistant)
Describe Hib vaccine
Conjugate vaccine w/Type B polysacch
Aspiration pneumonia, Abscess in lungs, Alcoholics, diAbetics” think....
Klebsiella pneumoniae
CXR presentation of Klebsiella
CRX: upper lobe w/bulging fissure – classic presentation
Sputum in Klebsiella
red curant jelly
This bacteria commonly follows H. Influenzae
S. aureus
Staphylococcal
Pneumonia
Pneumonia with high fevers and fiegets sign think....
Legionella
CXR appears worse then symptoms, walking pneumonia think....
Mycoplasma pneumoniae
Pneumonia in college students think....
Chlamydophilia
Pneumoniae
Are the majority of bacteria in aspiration pneumonia anaerobic or aerobic.
ANaerobic AspiratioN
Nosocomial pneumonia is due to _____ gram ____ bacteria
aerobic gram negative
Characteristics of empyema

pH, glucose, gram stain, ldh
low ph, low glucose, gram positive, high ldh
Neisseria mode of metabolism and appearance
aerobic gram negative diplococci
Confirmation of the presence of Neisseria is based on a series of ________ fermentations
carbohydrate
Serogroups of N. Menigintidis based on
capsular polysacchiride
Serotypes based on
outer membrane protein
Only bacterial meningitis associated with epidemics?
Meningococcal Meningitis
Most cases are due to which serogroup
b
Most cases of meningitus are caused by
strep pneumoniae, most common cause of adult menigitis
petechial rash think....
Meningococcemia
Clinical manifestations of Meningococcemia
aprehension, delirium, rapid progression, shock, high mortality
Neck stiffness think...
meningitis in adults, young children may only have fever and vomiting
Classic medium for culturing Neisseria is?
Thayer-Martin, chocolate agar with antibiotics that kill competing bacteria.
Treat Neiserria meningitidis with
cephalosporins
Who gets meningococcal chemoprophylaxis?
only those that come into close contact, give rifampin, ceftriaxone, or cipro
Function of por protein (1) in N. Gonorrhoaea
prevents phagolysosome fusion in neutrophils
Function of Opa Protein (2)
mediates attachment to epithelial cells and invasion into cells
Function of lactoferrin binding protein
abstracts iron
Pathogenesis of N. Gonorrhoae
ciliary stasis
death of ciliated cells
attachment
internalization
intracellular replication
intracellular traffic
exocytosis
N. Gonorrhoae infection in men restricted to
urethra
N. Gonorrhoae infection in women restricted to
cervix
Pustular skin lesion think
N. Gonorrhoae
N. Gonorrhoae treatment
ceftriaxone
Moraxella commonly seen in patients with?
COPD
Otitis media and Sinunitus think?
Moraxella catarrhalis
Treament of Moraxella
very resistant, use broad spectrum
- Lactose fermenters
SCEEK
Serratia, Cirobacter, E. coli, Enterobacter, Klebsiella
Salmonella, Shigella & Pseudomonas aeruginosa DO NOT
- MacConkey Agar
- Lactose fermentors  RED – colonies are red at low pH
Enterobacteriacea generalities
-Doesn’t cause sore-throat
-Think COFFEe
Capsular
O antigen – polysach. of endotoxin
Flagellar antigen
Ferment glucose
Enterobacteriacae
Most common cause of UTI, and travelers diarrhea (adults)
E-Coli
Pneumonia – debilitated PTs
(Red currant jelly sputum cavitary), think
Klebsiella
Bright Red Pigment produced by prodigiosin think
Serratia (Lactose fermentor (SCEEK)
slow fermentor)
-Model: airborne dissemination of bioterrorism
-Can survive some disinfectants & non-bacterial soaps; related to outbreaks
Plague (bubonic, septicemic, and pnemonic)
Yersinia Pestis
OUTBREAK: blood bank, chocolate milk
Yersinia Enterocolitica
Yersinia staining uniqueness?
bipolar staining
ONLY seen in hospital think...
Acinetobacter
-Most frequent wound infection following cat/dog bite/scratch think...
Pasteurella mulocida
Outbreaks cruise ships (japan) think....
Vibrios parahaemolyticus
leeches think...
Aeromonas
New Orleans, oysters – alcoholics (cirrhosis ) think...
v. vulnificus, rapidly progressive cellulitis with bacteremia, abrupt onset fevors, high mortality
Unpasteurized milk, Undulant fever, feral pigs, animal workers think
Brucellosis
Where does Brucellosis live and multiply?
Facultative intracellular parasite – multiplies in macrophages. (requires cell-mediated immunity to control), first infects lymph nodes, disseminates to liver, spleen, kidney
Transmitted via cat flea, Bacillary angiomatosis, think
Bartonella henselae
Trench fever think
Bartonella Quintana
Andes mountains, Oroya Fever,
Bacteria penetrate RBCs (like malaria) – Carrion’s Disease:
Bi-phasic
-Acute: Fever, myalgia, headache
-Chronic: Verruga peruana (cutaneous nodules) after 1-2 mos; may persist for yrs
Bartonella baciiliformis
Psedomonas aeruginosa
Simon
Metabolism, fermentation? oxidase? pyocynanin?
-Gram (–) Bacillus
-Nonsporulating
-Obligate aerobe except w/nitrate.
-Non-fermentor
-Oxidase (+) – has cyt oxidase a3.
-Pyocyanin blue-green
“Be Pseudo"
Burns
Endocarditis (IVDA)

Pneumonia (CF)
Sepsis (↑mortality)
External Otitis (malignant)
UTI
Diabetics get..
Osteomyletis
#2 nosocomial pathogen
Pseduomonas, #1 is E.Coli
4 predisposing factors,
immunosupression, icu, cystic fibrosis, prolonged hospitalization
Colinization
found on skin, moist environments
Function of exotoxin A
inhibits protein syn. (ADP ribosylation of EF-2
Function of exoenzyme S
: inhibits proteins syn.; promotes dissemination (tissues
What allows it to produce reactive oxygen species?
pyocyanin
How does it protect itself from radicals
superoxide dismutase
lesions on butt?
Whirl Pool Folliculitis
Neutropenic Cancer PTs are prone to?

SPEK
E. Coli
Klebsiella
Pseudomonas
S. Arueas
-Endocarditis in IV drug users (Detroit). think....
Think Psuedo
Uti Predisposing factors 4
anatomic (short urethra), sex, foley cathetars, nosocomial
most abundant protein in urine: binds E. Coli and prevents epithelial cell attachment.
Tamm-Horsfall protein
: recognizes bacterial polysaccharides; abundantly expressed in bladder & kidney
TLR11
most common route of infection, ascending or descending
ascending is most common: rectal flora  perineal colonization  vaginal colonization  introital colonization  urethra  bladder ( kidney).
introital colonization is associated with?
vaginal pH, & ↑’d w/antibiotics (esp β-lactams).
Community aquired UTI is most likely
Ecoli or Saprophyticus
Hospital aquired UTI is most likely
Pseudomonas
Is preexisting antibody to a UTI infection preventative?
-pre-existing Ab to the infecting org is NOT protective, immune system has little role in UTIs: NO increased incidence of UTIs in patients w/humoral or cellular immune deficiencies
Most correlated with antibody response, upper tract or lower tract infection?
upper
WBC casts think...
pyelonephritis upper tract
What bacteria causes renal failure?
bacterial infections don’t cause renal failure unless there is an obstruction.
Who should not be treated for asymtomatic bacteruria
don’t treat in adults (esp elderly) b/c tx associated w/higher mortality, treat in pregnant women and kids
T/F ALL UTIs are ANATOMICAL
T
Acute Bacterial Prostatitus most commonly caused by
E.Coli
Prostatitis symptoms
fever, bank pain, chills
appearance of prostate
warm, swollen, tender
T/F you should massage a prostate that is inflammed.
NO PROSTATE MASSAGE
How is chronic prostatitus different from acute?
non-bacterial
may be asymptomatic
massage may be done
Problems with Prostate treatment
hard to penetrate prostate, treat for 3 weeks.
Fever
Fever
Signals received by the pre-optic nucleus are sent from the _____ hypothalamus to the _____ hypothalamus – location of the hypothalamic set point
anterior to posterior
example of a pyrogen
LPS
example of endogenous pyrogen 3
Il-1, TNFa, IFNg
normal fever range
35.6 – 38.2
two pathways to fever
1) IL-1 ant hypothalmus, protaglandins, post hypothalmust, change set point

2)local inflammation, thermosensitive neurons, signal to ant hypothalus
In what situation is it better to treat the fever over the infection
if a cns injury is present
Fever of unknown origin
> 3 weeks of fever (>38.4oC)
Infection most common cause
Avoid “therapeutic” trials of antibiotics
Make a diagnosis first
Acetaminophen in children may promote
Reyes syndrome
_
_
_
_
Sepsis
Sepsi
Define Bacteremia
bacteria in blood (bad)
Define Sepsis
Bacteremia PLUS signs of systemic inflammatory response
Fever, tachycardia, hypotension, etc
Define Septic Shock
Sepsis PLUS signs of end-organ hypoperfusion
Septic shock more likely to be cause by Gram- or Gram+
Gram Negative
Sepsis caused mostly by Gram- or Gram+
Gram +, Used to be Gram -
Most common cause of bacteremia?
Ecoli
Most common cause of death due to bacteremia is caused by what organism?
Psuedo due to debilitated host
the TWO MAIN mediators of the pro-inflammatory response in sepsis.
Interleukin-1 (IL-1)
Tumor necrosis factor-α (TNFα)
Toxins that cause sepsis similar to LPS are known as?
Superantigens
D-dimers are markers for what process?
DIC
DIC is treated with?
anticoagulative factors
Describe the anti-inflammatory response seen in sepsis
Shift from a Th1 to a Th2 response to infection
See apoptosis of lymphocytes and secondary infection due to immunosuppresion
Clinical Manifestations of Shock on cardiovascular system 4
reduced tpr
Compensatory increase in cardiac output
Direct suppressive effect of LPS on myocardial contractility
Shock results when heart cannot compensate for reduced SVR
Shocks effect on respiratory system
Acute Respiratory Distress syndrome which is caused by increased microvascular permeability
What is an anticoagulant used to treat sepsis?
Protein C
A prime function of IL-1 and TNFα
up-regulate adhesion molecules in neutrophils and endothelial cells and cause their activation
upregulation of this stimulates coagulation
tissue factor
Gram negative most common site of infection
UTI
Organs most affected by hypotension in order from first to last
Hypotension first hits the kidneys, then the lungs, then the gut, then the heart, brain
What Interlekins shift TH1 to TH2
Il-6 and Il-10
Most common bug acute endocarditus
Staphylococcus aureus most common bug
Most common bug subacute endocarditus
Viridans Streptococci
Endocarditus pathogenesis, 2 steps
non-bacterial thrombotic endocartitus, transient bacteremia
Strep. bovis: think....
Colon Cancer
Prosthetic valve endocarditis causes, 2, one is early, one is late
Early (<60 days post-surgery): S. aureus, CNS
Late: viridans Streptococci
Valve commonly effected in IV drug uses?
tricupsid (right heart)
IV drug users are susceptible to what bacteria? 2
s. aureus, pseudo
Classic presentation of endocarditis
fever, anemia, changing murmor
Peripheral Manifestations of Endocarditis beginning with, C,S,O,J,R
Conjunctival petecchiae
Splinter hemorrhages: nail beds
Osler’s nodes: painful nodules on pads of fingers and toes
Immune complex vasculitis
Janeway lesions: non-tender erythematous macules on palms and soles
Micro-abscesses
Roth’s spots: retinal hemorrhages
Immune complex vasculitis
Three lab finding in endocarditis beginning, A,T,M
Anemia
Thrombocytopenia
Microscopic hematuria
Echocardiogram with more sensitiviy for detecting endocarditis
Transesophageal (TEE) has higher sensitivity than transthoracic (TTE) therefore is preferred
Two categories of people that should be given antibiotic prophylaxis for endocarditis
those having dental procedures,

and Give to high risk ONLY:
Prior endocarditis
Prosthetic valve
Heart transplant with valve abnormalities
Complicated congenital heart disease
What time should surgery be performed in a patient with endocarditis that requires surgery?
ASAP
-
-
-
-
Gram Positive Rods
Gram Positive Rods
Bacillus antracis spore forming or non spore forming
spores
Three virulence factors of anthrax
lethal toxin, edema toxin, capsule
CXR or anthrax
wide mediastinum
Clinical phases of Respiratory Anthrax
initial 1-3 days - maliaise
secondary 1-2days, sudden progression to death
Two types of GI Anthrax
abdominal and oropharnygeal
Best way to control antrax
controll it in animals first
Bacillus cereus spore forming, or non spore forming
spore forming
Rice contaminated with bacteria think
Bacillus ceres
Two types of B. Cereus food poisoning
Emetic and Diarrheal
C. diptheriae shape on gram stain
club shaped
C. diptheriae transmission
airborne droplets, colder months, crowded conditions
C. diptheriae produces inflammation first in what location
exudate in pharynx/trachea
Soft tissue of neck swells, called a bull neck.... think
C. diptheriae
describe C. diptheriae exudate
tough, leathery, grey
C. diptheriae will travel two places after setting up camp in the pharynx
cardiac toxicity, neurologic toxicity
Listeria monocytogenes mode of transmission
veritical
Population at risk for Listeria monocytogenes
old, young, pregnant, immunosuprresed
Listeria monocytogenes incubation period
11-70 days
Listeria monocytogenes is enhaced by what medication
H2 blockers
Listeria monocytogenes lives
intracelluarlly
What is Granulomatosis Infantiseptica and what causes it
Listeria monocytogenes, granulomas in infants
Swine worker, skin lesion, diffuse rash, endocarditus think....
Erysipelothrix
95% of anthrax infections are of what manifestation
cutaneous
Bacillus ocular infections are caused by antracis or cereus, and what patient population is at risk
cereus, IV drug users
Bacillus Cereus rice causes
emetic food posinening
Bacillus Cereus meats or vegetables cause
diarrheal food posinening
C. Diptheriae rods are shapped like?
clubs, chinese characters
C. Diptheriae transmission takes place via?
airborne droplets
Listeria Mono horizontal transmission occurs via
zoonosis, unpasterized milk
Listeria Mono lives inside what type of cell?
Macrophages
Mycoplasma P. pathogenesis
affinity for epithelium, contributes to loss of cillia from host
Mycoplasma and Ureaplasma appearance on agar
fried egg, except Mycoplasma P. which has a mulberry appearance
Mycoplasma P. is spread via
aerolized droplets, single cases or mini epidemics are common
Mycoplasma P. two clinical presentations
Tracheobronchitis and atypical pneumonia
Mycoplasma P. feature that may distinguish it from other atypical pnuemonias
bullous myringitis
What is responsilbe for causing Raynauds Phenomenon in Mycoplasma P.
cold agglutinins
Chlamydia morphology
gram negative, intracelluar
Chlamydia form that is infectious
elementary body
Chlamydia form that replictates
reticulate body
Chlamydia trachomatis clinical manifestations,
Urethritis/Cervicits
Lymphogranuloma venerum
ocular infection
Chlamydia trach infects what type of cells
non-cilliated columnar epithelial cells
birds are natural resovior, pneumonitis think
Chlamydia psittaci
Bordetella Pertussis transmitted via
inhalation of aerolized bacteria
Bordetella Pertussis attached to what cells
cilliated epithelial cells of the bronchial tree
3 viruluence factors of Bordetella Pertussis
Filamentous hemagglutin - blocks phagocytosis, binds to cillia

Pertussis toxin - increases mucus secretion, prometes leukocytosis,

Bacterial pili/pertactin - attachment of bacteria to respiratory epithelium
4 stages of Bordetella Pertussis
incubation 7-21 days
catarrhal 1-2wks malaise
paroxsymal 2-4 weeks whoop
convalescent month pneumonia/cns
Bordetella Pertussis main cause of mortality
complication from aspiration pnemonia
Haemophilus influenzae growth requires what 2 supplements
x factor and v factor
HIB vaccine is made against what strain of Haemophilus influenzae
B type capsule
Haemophilus influenzae transmission occurs via
contact secretions or airborne droplets
Haemophilus influenzae virulence factors 4
capsule
LPS
Outer membrane proteins - iron scanvanging
Fimbriae - enhance adherance present in all types
Clinical syndromes of Haemophilus influenzae
5
Arthritus
epliglotitus
meningitis
otitis media
sinusitis
Haemophilus influenzae sinusitis is caused by what type of Haemophilus influenzae
non-encapsulated strain
most common cause of otitis media in children
Haemophilus influenzae
std common in men, tender papule with erthematous base on the genital or perianal area think
H. ducreyi, Chancroid
Legionella pneumophila morphology
gram neg, aerobic, intracellular
Legionella pneumophila grows on this media
charcoal yeast enriched media
Legionella pneumophila most cases are in
summer/fall
Legionella pneumophila pathogenesis
engulfed by macrophages
multiplies
macrophage is killed
bacteria release
Legionella pneumophila clinical manifestation
pulmonary infiltrates multi-lobar
DIC
pleuritus, empyema, cavitary lung disease

essentially intense inflammatory changes
Legionella pneumophila, extrapulmonary legionellosis occurs in?
immunocomprimised hosts via bacteremic spread
-
-
-
-
-
-
Skin/Soft Tissue /Bone
Skin/Soft Tissue /Bone
When dealing with skin infections always keep what bug in mind
Staph A. and MSRA
Impetigo site, bugs, and clinical features
intraepidermal, Group A strep, Staph A, "golden stuck on crusts"
Folliculitis site, bugs, and clinical features
hair follicule, staph a, pustules
Furuncles site, bugs, and clinical features
hair follicule, staph a, deeper nodule that becomes an abscess
Carbuncle site, bugs, and clinical features
hair follicule/subcutaneous fat, staph a, acutley ill, develops from furnuncle, multiple drainage sites for pus
Ecthyma, site, bugs, and clinical features
penetrates epidermis and dermis, group a strep, punched out uclers
Erysipelas site, bugs, comments
Superificial cellulitis with lymphatic involvement, GAS, Lymphatic obstruction is risk factor
Sharp demarcation of bright red lesion
Cellulitis site, bugs, comments
Cut and subcut, GAS, MRSA, Spreading infection
portal of entry
associated lymphangitis with GAS
Necrotizing fasciitis, site, bugs, comments
Subcutaneous soft tissues, fascia, muscle

Type I: polymicrobial (anaerobes, GNB, non-A Strep)

Type II: GAS

LIFE-THREATENING!!
Pain >> physical findings
Rapidly progressing
Urgent surgical intervention
Purplish bullae
Fournier’s gangrene: scrotom/perineum
Clostridial myonecrosis site, bugs
skeletal muscle, Clostridium perfringens
“Gas gangrene”
Contaminated wounds
LIFE THREATENING
Severe pain
Skin crepitus
Foul odour
Gas on plain Xray
Clostridial myonecrosis from C. Perfringens
Osteomyelitis – hematogenous
most common bugs in adults 3
staph a, strep group a or b
Osteomyelitis – hematogenous
most common bug in infants
E-Coli
Osteomyelitis – hematogenous
most common bug in children
H.Flu
Osteomyelitis – hematogenous
most common bug in IVDU
Pseudo
most common etiology of osteo in kids
Hematogenous spread from trauma
Osteomyelitis – contiguous focus no vascular insufficiency common bug and usual cause
Staph A., direct inoculation after trauma, decubitus ulcers (bedsores)
Osteomyelitis – contiguous focus with vascular insufficiency bugs and usual cause
polymicrobial, minor trauma, common in diabetics
Septic arthritis common bug infants 3
Group B strep, GNB, Staph A.
Septic arthritis common bug Adolescents
N. gonorrhoeae
Septic arthritis common bug adults
Staph a.
A patient with septic arthritis experiences pain upon what type of joint motion
extension
Septic Bursitis common bug
Staph A.
Septic Bursitis patients experience pain on what type of motion
flexion