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3 Cards in this Set
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Recombinant DNA (rDNA) |
*taking DNA from different organisms and combining it *usually incorporating foreign DNA into a plasmid *plasmid = vector *gene of interest is cleaved by enzyme, inserted in plasmid, taken up by bacterium, and that bacterium is cloned Ex: Human growth hormone, insulin |
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5 tools of biotechnology |
1. selection 2. mutation 3. restriction enzymes 4. vectors 5. PCR (makes many copies of DNA) |
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Restriction Enzymes |
*cut DNA at specific points *common in bacteria *use same enzyme to cut donor & recipient DNA *Use specific set of bases as code to cut (blunt ends & sticky ends) |
