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24 Cards in this Set
- Front
- Back
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Which of the following is largest?
nanometer decimeter micrometer millimeter |
decimeter
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A millimeter is?
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1/1,000 of one meter
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0.000003m (or 3x10^-6) is equivalent to?
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3 micrometers
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What unit of measure is used to measure the size of viruses and cellular organelles?
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nanometer
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How is an object magnified by light passing through a lens?
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Lens refracts light rays and focuses them on a focal point; the opject appears larger as the rays of light pass through the focal point
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Define wavelength?
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distance between two corresponding parts of a wave of radiation
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What do you call a microscopes ability to distinguis between two objects that are close together?
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resolution
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What quality of lenses used in a microsope improves resolution of the microscope?
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numerical aperture of the lens
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Contrast is best described as?
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differences in intensity between an object and its background
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What type of microscopy is most common in microbiology laboratories?
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bright-field
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What is an advantage of using dark-field microscopy instead of a bright-field?
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contrast is better and allows better visualization of details
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What type of light microscopy increases the contract to such an extent that the image of the specimen appears three-dimesional
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differentail interference contrast
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What type of light microscopy is used to view the interal structures of microbes?
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phase-contrast
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What type of microscopy uses an electron beam to create a three-dimensional image of cell surfaces?
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scanning electron
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Which of the following is NOT a reason why scientists use stains to view microbes?
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to observe the specimen while it is still alive
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Which of these is NOT a differential stain?
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flagellar
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Differential staining can be used for what? (three things)
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-differentiate types of cells
-differentiate structures within cells -detect presence of certain chemicals in cells |
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Why are some bacteria, such as genera Mycobacteria and Nocardia, NOT effectivel stained with Gram stain?
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they have large amount of waxy lipid in their cell walls
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If a student accidentally skipped the destain step, what color would E. coli appear? (typically gram negative)
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purple
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What taxonomic categorie is most broad?
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phylum
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what is the basis for assigning organisms into domains?
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ribosomal nucleotide sequences
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When performing phage typing, how would one know whether the bacterium is killed by any of the bacteriophages?
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by the formation of a plaque
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What is one way microbiologists can distinguis between different bacteria that are similar to mophology and staining characteristics?
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biochemical testing
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Which two domains in the "3-domain system" are withing the kingong Prokaryotae of the "5 kingdom system"
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Bacteria and archaea
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