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15 Cards in this Set
- Front
- Back
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Light microscopy |
A typical bright field microscope such as used in lab |
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Oil immersion and light refraction |
Different media air, water, glass, oil. Bend the light to different degrees |
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Bright vs. Dark field microscopy |
Only light reflected by the specimen will enter the objective lens |
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Phase contrast microscopy |
Reveals internal detail without staining. Useful for live specimens |
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Differential interference contrast microscopy (DIC) |
A variation on phase contrast microscopy involving a more complex combination of filters and prisms. Create an image with even greater detail and contrast. Image has a three-dimensional appearance as if it was eliminated from the side. |
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Fluorescence microscopy |
Fluorescent dyes or antibodies with the fluorescent tag stick to specific targets. Under UV light, dye fluorescences, only labeled cells or structures are seen |
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Confocal fluorescence microscopy |
Only light from a given depth or plane is transmitted, out of focus light is excluded |
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Electron microscopy |
Electromagnetic lenses focus electron beam onto metal stained specimen. Allows for greater resolution then with light microscopy. |
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Simple stain |
Dye that nonspecifically stains all organisms, features |
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Differential stain |
Dye that binds various structures or organisms differently |
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Counterstain |
May 2nd dye added that is a different color than original dye |
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Negative stain |
Dye that stains backgrounds not specimen |
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Special stain |
Dye that specifically stains certain subcellular structures |
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Mordant |
Is any chemical added to enhance stain |
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Taxonomic hierarchy |
Domain. Kingdom. Phylum. Class. Order. Family. Genus. Species. |
