Micro 140 Written Final

Front 1

40x lens can get oil on it

Back 1

true

Front 2

the shorter the wavelength the better the resolution

Back 2

true

Front 3

agar is made from the cell walls of marine algae, is solid at room temp neither repulsive or attractive to microbes, we then add components to grow microbes

Back 3

true

Front 4

Light does what

Back 4

1. limits resolutiondue to its sizeand so it limits magnification.
2.is larger than electrons and gives less resolution and magnification than electrons for this reason.
3. is focused on the specimen by the substage condenser.

Front 5

The iris diaphram is:

Back 5

1. part of the lens system of a compound light microscope.
2.can be used to enhance or vary brightness in a compound microscope.
3.is part of the illumination system of a compound microscope.

Front 6

The lens system contains:

Back 6

1. the objective lens
2.contains the ocular or eye piece
3.contains immersion oil
4. contains electromagmentic lenses in an electron miscroscope.

Front 7

state the prefix
1. 3000
2. .08
3. .000008
4. .008

Back 7

1.kilo
2.centi
3. micro
4. milli

Front 8

what level of biohazard is the lab

Back 8

level 2

Front 9

why use tsb

Back 9

to culture one type of bacteria or grow anarobes.

Front 10

where is bacillus antraxis found and is it a pathogen
what are its characteristics and how would you treat it.

Back 10

1.It can be found in air , soil, inside people, food, cut. It is a pathogen.
2. treatment- penicillin inhibits the enzyme that helps in cell wall production.

Front 11

what is listeria and how is it treated?

Back 11

infection occurs through digestion. it causes fever muscle aches and and diarrea.
2. treatment- gram pos bacteria treat with penicillin.

Front 12

Soil contains:

Back 12

1. Pseudomonas aeruginosa
2.contains agents that can be brought into hospitals on flower arrangements and cause special problems in burn wards.
3. contains endospore forming organisms

Front 13

colonies:

Back 13

on a plate can be counted to find out the original number of organisms in your sample since one organism gives rise to one colony

Front 14

how many micrograms are in 5 grams of salt

Back 14

5,000,000 ug

Front 15

how many milliliters in 1 liter

Back 15

1000 ml

Front 16

how many microliters in 1 milliliters

Back 16

1000 ul

Front 17

what are the physical and chemical properties of skin

Back 17

1.the skin epidemins is made up of dead karatin cells on the top layers
2. its dry and acidic
3.sebum

Front 18

who is dr semmelweiss

Back 18

he introduced handwashing to medical procedures.

Front 19

what is a nosocmial infection
name an organism

Back 19

1. A nosocmial infection is one that is aquired at a hospital.
2.staph aureus causes infectons and disease if entered inside a would or internal to body like bones.

Front 20

why was the handwashing expirment an expiriment

Back 20

1. because there was a control and variables in the test.
2. allowed information to be obtained from a hypothisis.

Front 21

what is the relationship between OD and %T

Back 21

1. Percent T is the ratio of the amount of light passing through the sample to the light applied to the sample mult by 100.
2. OD is optical density it represents the concentration of solute in log base 10 form
3. relationship is becasue OD is calculated using % T.
4. when using calibrate to blank tube first.

Front 22

Why use spectrometry

Back 22

it shows the absorbance of the solute.

Front 23

what is microbial opportunist and microbial antoagonism

Back 23

1. opportunist microbes that find and adapt to microbe communities.
2.microbes that fight to dominate or join microbe communities.

Front 24

what is gambierdiscus toxicus

Back 24

it is the protist that causes ciguatara

Front 25

what depths are red, green, and brown algae found

Back 25

red lives deep down
green on top in tide pools
brown- intermediate depth

Front 26

what is mycelium

Back 26

it consists of a lot of hyphae

Front 27

what is the difference between sporangia and conidia

Back 27

sporangia is a round ball with spores inside. conidia has spore fingers branching off.

Front 28

what are the bubbles that come out of yeast

Back 28

it comes from fermentation that causes co2

Front 29

name a couple helmiths

Back 29

round and flat worms

Front 30

algae characteristics are

Back 30

1. are photosynthetic eukaryotes that looses electrons when kight strikes and accessory pigments that differ in red, green, brown as well as dinoflagellates.
2.grow where they have pigments to absorb the available light.
3. have light dependent and indepentdant reactions like plants.

Front 31

In photosynthesis

Back 31

1.co2 is the source of carbon for glucose which provides energy for much of the biosphere directly or indirectly.
2.light and water in the presence of chlorophyll a and other pigments is harvested into ATP and NADPH.
3. Carbon from CO2 is fixed into glucose.

Front 32

photosynthesis

Back 32

1. chlorophyll a is common to both photo synthetic bacteria, algae and terrestrial plants as the molecule that looses its electrons when light strikes directly or indirectly.

Front 33

name a type of fungi

Back 33

rhizopus

Front 34

what is a bacteria found in milk

Back 34

listeriam staph aureus

Front 35

what is an organism found in poultry

Back 35

salmoneela

Front 36

where can cholera be found

Back 36

tofu water, poi,

Front 37

what organism can be found on beef

Back 37

escherichia coli

Front 38

how can you treat a endotoxin caused by botulism

Back 38

with an anti-toxin

Front 39

what is the difference between internsic and extrinsic preservation methods

Back 39

internsic is factors inherant in food,.
extrensic is enviromental factors

Front 40

what is a capsule made oF

Back 40

it is made up of a polysaccarides or polypertides used for protection and adhesion.

Front 41

is an endopsore a vegitative cell

Back 41

its in a dormate state but when the conditions are right it will turn into a vegitative cell.

Front 42

Does bacillus megaterium form endospores

Back 42

yes

Front 43

does klebsella pnemonia form capsules

Back 43

yes

Front 44

how does tetracycline work

Back 44

it inhibits protien sysnthesis

Front 45

what are the sub units of polysaccharides

Back 45

monosaccarides / disaccarides

Front 46

what are the sub units of protiens

Back 46

amino acid poly peptides

Front 47

what are the sub units of lipids

Back 47

gyserol, fatty acids

Front 48

what indicator was in the sprig experiment

Back 48

phenol red