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27 Cards in this Set
- Front
- Back
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1) Who invented Aseptic
technique? 2) What is Aseptic Tech? |
1)Robert Koch
2)Minimize contamination, kill as many organisms as you can. |
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List 3 reasons we use Aseptic Technique in the lab?
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1) Protect our experiment
2) Prevent contaminating you. 3) Prevent lab contamination. |
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What type of sterilization tecnique do we use in the lab?
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DRY HEAT which uses "oxidation"
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What type of microscope do we use in the lab?
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Compound light microscope
* It has more than 1 lens * 1,000 is highest magnify * oil emersion/decreasing your refractive index so light does not bend. |
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Genetic Transformation
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in nature/ in living cells
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congregation
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pillis/transfer plasmid
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recombient DNA
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An organisim from 2 species
We used ecolii and jellyfish |
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We used a Plasmid
that carried what? |
extra chromosomal DNA
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we used I or --- gene
transformation? |
horizontal
during their life cycle they can change their GENO |
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PLASMID
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Vector that carries genetic material
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With genetic transformation, we increase our odds by doing what?
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changing the temp (Heat Shock) Temp allows plasmids to enter.
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Selective Agent "Media"
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where the only one that grew was the one that glowed.
We changed weather or not the gene was turned on. |
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What was the trigger that we used to turn on the gene for genetic transformation?
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SUGAR/ aribinase
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Define PCR
How does work? |
Pliminary Chain Reaction
1)protected DNA in our cells 2)Softened membrane/temp 3)bursted open membrane/temp |
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PCR Steps
How many cycles did we run? |
A) DENATURATION
B) ANNULING C) EXTENSION 40 Cycles= 1.1 Trillion |
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In PCR LAB what did we use?
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1) yellow dye
2) Enzyeme (pilmirase/non human) 3) Nuclea tides |
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How does electrophoesis
work? |
It takes a negative DNA charge and runs it towards a positive. The smaller pieces go further.
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What is the purpose of a loading DYE in the PCR lab?
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A loading Dye is a heavy liqued that helps us visulize and helps DNA stay in the wall.
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PHAGE
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virus that attacks a bacteria
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What do restriction enzymes do?
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They are specific and they cutt and paste sequences of DNA
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What is important when you are preparing a smear?
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1) spread the smear thin
2 )Heat fix the smear to kill an organism and it sticks the cells onto the slide 3) There are two smears: LIQUED= use 2 loopfulls SOLID= 1 loopfulls |
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STEPS in doing a
GRAMSTAIN |
1)Make smear(Crystal Violet)
2)Add Iodine(Mordant) which attracts stain to cell and makes it bigger. 3) ADD decolorizing Agent (Ethyl Alochol) 4) ADD Safarin |
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GRAM + stain
VS. GRAM - stain |
GRAM + (purple) thick peptiglyed cell wall
GRAM - "pops it out" "Colorless" |
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In the SPORE STAIN:
What color was the spore? WHat did we use? |
GREEN and we used moist heat
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WHat temp do organisms mostly like to live in?
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37 degrees
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What % of alcohol works best?
In our tests that we ran, what didn't kill anything? |
70% alcohol worked best and also 10% of bleach didn't work well
hand sanitisers, clorox wipes didn't kill anything. |
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Every living organism has what?
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PH and a TEMP that it likes to live in. 37 is ideal for our labs.
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