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50 Cards in this Set
- Front
- Back
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deals with the study of tiny living organisms |
Microbiology |
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an optical instrument that is used to enlarged small objects |
Compound Microscope |
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2 Lens System |
Objective lens Ocular lens / Eye piece |
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total Magnification |
ocular lens ×× objective |
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refers to the ability of the lenses to distinguish two separate objects at a specified distance |
Resolution |
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3 Parts of a Microscope |
Mechanical Magnifying Illuminating |
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Mechanical Parts of a Microscope |
base arm body tube revolving nosepiece course&fine adjustment |
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Magnifying Parts of a Microscope |
Ocular Lens Objectives |
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Kinds of Objectives |
Low Power Objective (LPO) High Power Objective (HPO) Oil Immersion Objective (OPO) |
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Illuminating parts of a Microscope |
Mirror Diaphramn Condenser |
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Cocci |
spherical/violet |
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Algae |
Green |
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Fungi |
filamentous/blue |
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Virus |
Green |
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ciliates |
irregular shape/grayish |
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Yeast |
oval/colorless/transparent |
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Spore |
cell body/blue |
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flagella |
red |
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Major Parts of Microorganism |
Acellular Cellular |
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Acellular |
Virus Prions Viroids |
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Cellular |
Prokaryotes Eukaryotes |
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Prokaryotes |
Archaea Bacilli Cyanbacteria |
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Eukaryotes |
Algae Bacteria Protozoa |
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the nutrient preperation for culturing microbes |
Media |
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Solodifying Agent |
Agar |
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Cultured Agar |
Agar broth deep slant plate |
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Kinds of Sterilizing |
autoclaving dry heat sterilization UV radiation |
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Aseptic Transfer |
Sterilizing - Autoclaving Inoculating Loop/needle |
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salts that are acidic , basic , and neutral |
Stains |
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one of the ions that impart color |
chromophore |
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cytoplasmic stains |
Acidic dyes |
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have ni chromophore that is positively charge |
basic/nuclear stains |
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mixture of acidic and basic stain |
Neutral stain |
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Kinds of Staining |
Simple Stains Differential Stains Special Stains |
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use a single/basic dye to color/highlight the entire organism |
Simple Stains |
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use to determine the reaction of bacteria to a particular stain |
Differential Stains |
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used to color a specific part of a microorganisms (ex. endospores , flagellum , capsule) |
Special Stains |
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Prepared Slide |
Smear |
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it classifies bacteria into two groups |
Gram Staining |
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Gram staining |
1884 Hans Christian Gram |
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Gram + (color) |
crystal violet |
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Gram - (color) |
Red |
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Ex. of Gram + |
Bacillus Clostriduim Corynebacterium |
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Ex. of Gram+ |
Borrelia Enterobacta Escherichia |
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it is more complex; more reliable observation of motility |
Hanging Drop |
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tends to dry quickly ; simplier to perform |
Wet mount |
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useful for short term observation |
Wet mount |
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useful for longer-term observation |
Hanging drop |
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vibrate ; move little |
Brownian Motion |
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move ⬆⬇⬅➡↗↖↘↙ |
True motility |
