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13 Cards in this Set

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How many microns are each ocular division on the respective settings?
4x, 10X, 40X, 100X
4x=25 microns
10X= 10 microns
40x= 2.5 microns
100x = 1 micron
3 steps for wet mount:
1-place drop of water on slide
2-place specimen in water,
3-drop cover slip onto drop of water
3 steps for hanging mount
1-place coverslip on table
2-place liquid w/ microorganism on cover slip
3- press upside down slide onto slip
Steps to Simple and Differential Gram Stains
1.the smear is flooded with crystal violet for 60
seconds
2.rinsed with distilled water (End of Simple Stain steps)
3.flooded with an iodine solution for 30 seconds
(which is a mordant and forms a complex with
crystal violet)
4.rinsed
5.flooded with the 50-50 acetone alcohol for 3 to 4 seconds
6.rinsed
7.counter stained with Safranin for 45 seconds
8.rinsed.
why does Decolorizer wash crystal violet iodine from gram-negative cells?
it's 25% lipid walls, and 1% lipid is in gram-positive.
Gram-postivies ____ (more/less) susceptible to penicillin and are _____ (more/less) likely to be inhibited by dyes
more
more
Gram-positives have a more _____ nutritional requirement and are _____(more/less) susceptible to physical disruption.
complex
less
Gram-Negatives are ____ (more/less) susceptible to penicillin and are ____(more/less) likely to be inhibited by dyes.
less
less
Gram-negetives have ____ (more/less) nutritional requirements and are ____ (more/less) susceptible to physical disruption.
less
more
Steps in the acid fast test:
1.the smear is flooded with hot carbol
fuchsin for 3 to 5 minutes over heat.
2.rinsed with distilled water
3.flooded with acid alcohol
4.rinsed with distilled water
5.flooded with methylene blue for 60
seconds
6.rinse with distilled water
Acid fast bacteria stain is used primarily for the detection of tuberculosis and leprosy? T/F
True
How is Negative staining is done?
1.mixing bacterial cells with india ink.
2.This suspension is smeared on a slide and allowed to dry.
What is negative staining used for?
Detecting bacterial capsules and for cells that are difficult to stain.