Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
13 Cards in this Set
- Front
- Back
|
How many microns are each ocular division on the respective settings?
4x, 10X, 40X, 100X |
4x=25 microns
10X= 10 microns 40x= 2.5 microns 100x = 1 micron |
|
|
3 steps for wet mount:
|
1-place drop of water on slide
2-place specimen in water, 3-drop cover slip onto drop of water |
|
|
3 steps for hanging mount
|
1-place coverslip on table
2-place liquid w/ microorganism on cover slip 3- press upside down slide onto slip |
|
|
Steps to Simple and Differential Gram Stains
|
1.the smear is flooded with crystal violet for 60
seconds 2.rinsed with distilled water (End of Simple Stain steps) 3.flooded with an iodine solution for 30 seconds (which is a mordant and forms a complex with crystal violet) 4.rinsed 5.flooded with the 50-50 acetone alcohol for 3 to 4 seconds 6.rinsed 7.counter stained with Safranin for 45 seconds 8.rinsed. |
|
|
why does Decolorizer wash crystal violet iodine from gram-negative cells?
|
it's 25% lipid walls, and 1% lipid is in gram-positive.
|
|
|
Gram-postivies ____ (more/less) susceptible to penicillin and are _____ (more/less) likely to be inhibited by dyes
|
more
more |
|
|
Gram-positives have a more _____ nutritional requirement and are _____(more/less) susceptible to physical disruption.
|
complex
less |
|
|
Gram-Negatives are ____ (more/less) susceptible to penicillin and are ____(more/less) likely to be inhibited by dyes.
|
less
less |
|
|
Gram-negetives have ____ (more/less) nutritional requirements and are ____ (more/less) susceptible to physical disruption.
|
less
more |
|
|
Steps in the acid fast test:
|
1.the smear is flooded with hot carbol
fuchsin for 3 to 5 minutes over heat. 2.rinsed with distilled water 3.flooded with acid alcohol 4.rinsed with distilled water 5.flooded with methylene blue for 60 seconds 6.rinse with distilled water |
|
|
Acid fast bacteria stain is used primarily for the detection of tuberculosis and leprosy? T/F
|
True
|
|
|
How is Negative staining is done?
|
1.mixing bacterial cells with india ink.
2.This suspension is smeared on a slide and allowed to dry. |
|
|
What is negative staining used for?
|
Detecting bacterial capsules and for cells that are difficult to stain.
|
