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18 Cards in this Set
- Front
- Back
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COMMON OBJECTIVES
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2.5-4X scanning
10-20X intermediate 40-45X high dry 90-100X oil immersion |
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POLARIZATION OBTAINED BY
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polarizer and analyzer
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TOTAL MAGNIFICATION OBTAINED BY
2 LENS SYSTEMS |
ocular x objectives magnification
ocular and objectives |
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CLEARANCE ANGLE
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3-8
angle between cutting facet of knife and block face, referred as knife tilt too |
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CLEARANCE ANGLE OR BLADE TILT TOO SLIGHT
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missed or skipped sections, alternately thick and thin sections
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CLEARANCE ANGLE OR BLADE TILT TOO GREAT
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chatter, microvibration, washboarding and undulations
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PARAFFIN MELTING POINT INCREASES
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paraffin harder, more support for hard tissue, easier for thin sections, hard to ribbon
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PARAFFIN MELTING POINT DECREASES
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paraffin softer, less support for hard tissue, harder to obtain thin sections, easier to ribbon
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HIGH PROFILE BLADE
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used for frozen sections
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TEMPERATURES
REFRIGERATOR FREEZER LABORATORY INCUBATOR FLOTATION BATH |
4
-20, long term storage -70 37 5-10 below melting point |
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PH MEASURES
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acidity or hydrogen ion concentration
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WASHBOARDING AND UNDULATIONS CAUSED BY
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worn microtome parts, cutting too fast or too large clearance angle
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PARCHET EARTH
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overdehydration or overstretching on waterbath
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ADDED TO SLIDES FOR ADHESION
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poly-L-lisine, chromate potassium sulfate, amino alkylsilane or positive charge slides
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ADDED TO FLOTATION BATH FOR ADHESION
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agar, gelatin and elmers glue
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TRANSMISSION ELECTRON MICROSCOPE USE WHICH KNIVES
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diamond knives
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PLASTIC EMBEDDED MATERIAL USE WHICH KNIVE
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glass knives
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PARAFFIN AND FROZEN KNIVES
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disposable blades or steel blades
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