Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
15 Cards in this Set
- Front
- Back
|
What is ELISA?
|
A solid phase immunoassay in which protein is absorbed to plastic and antibody-antigen interactions are qualitatively or quantitatively measured, based on light absorbance
|
|
|
What is Radioimmunoassay (RIA)
|
Essentially an ELISA that uses radioactivity in place of the enzyme to allow detection of the bound antibody
|
|
|
What is RIA useful for?
|
RIA is highly sensitive, so it is particullarly useful for the quantification of low-abundance substances
|
|
|
What is FACS (flow cytometry)
|
Most frequently used for the analysis of leukocytes, although any cell type that can be prepared as a suspension can be analyzed
|
|
|
What makes FACS (flow cytometry) powerful?
|
Ability to quantitatively analyze large numbers of cells, and to accurately identify complex or small populations. Also possible to separate specific populations of cells, based on specific phenotypes
|
|
|
What is immunofluorescence?
|
Similar to FACS, but fluorescence detection is by microscopy. Only a small number of cells can by analyzed
|
|
|
When would immunofluorescence be used?
|
To analyze or identify subcellular structures, to identify specific populations of cells, or to analyze a biopsy in which the available cell population is very small
|
|
|
What does Western blot do?
|
Fractionates proteins based on size
|
|
|
What is the Coombs test used for?
|
Testing for anti-Rh antibodies and to diagnose immune-mediated hemolytic anemia. Specifically detects antibodies against red blood cells
|
|
|
What is the direct Coombs test
|
Red blood cells are recovered from a patient via blood draw, and mixed with the reagent. Positive test is agglutination
|
|
|
What is the indirect Coombs test
|
Red blood cells are mixed with the patient and then with the Coombs reagent. Commonly used for pregancy
|
|
|
Name three sources of antibodies
|
Human Serum (used to measure a patient's antibody response)
Polyclonal antibodies (used as detection reagents) Monoclonal antibodies (hybridomas) |
|
|
What is a proliferation assay used for
|
To measure the ability of B cells or T cells to proliferate in response to mitogenic stimuli
|
|
|
What is a functional assay used for
|
To measure specific effector functions of leukocytes
|
|
|
Name two nucleic acid-based assays
|
Southern blot and Microarrays
|
