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29 Cards in this Set
- Front
- Back
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IgG
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37*C
Crosses placenta RBC |
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IgM
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4-22*C
NRBC |
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Zeta potential
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-difference in electrostatic potential between inner and outer layers of ionic cloud that surrounds red cells in solution
-enzs. removes salsilic acid |
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Albumin potentiators
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Increases dielectric constant
-Reduces zeta potential |
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LISS
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Low Ionic Strength Solution
-Decreases ionic strength of reaction medium -Reduces zeta potential Increases uptake of antibody molecules -Shortens incubation period |
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PEG
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Polyethylene Glycol
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DOSAGE EFFECT
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A phenomenon whereby an antibody reacts more strongly with a red cell carrying a double dose than with a red cell carrying a single dose of an antigen
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Location of ABO alleles
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Chromosone 9
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Codominant
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A and B
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amorph
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o
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Distribution O
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W:45%
B:49% A:43% |
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Distribution A
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W:41%
B:27% A:27% |
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Distribution B
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W:10%
B:19% A:25% |
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Distribution A,B
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W:4%
B:4% A:5% |
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ABO and H genes code for_____
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glycosyltransferases enzymes
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H gene
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-Chromosome 19
-affects the formation of ABO ag -Fucosyl= Transferase -fucose= sugar -must inharit H |
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Bombay phenotype
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No H gene inharited- hh(Oh)
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A gene
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N-acetylgalactosaminyl= trans
GALNAC= sugar |
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B gene
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-galactosyl= transferase
-galactose= sugar |
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O gene
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Just the H substances
-nothing extra |
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Type 1 precursor
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-found in secretions, body fluids, plasma
-beta 1--->3 linkage -glycoproteins |
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Type 2 precursor
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-found on RBCs
-beta 1--->4 linkage -glycolipids |
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Secretors
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H, A, B water soluble substances found in saliva
-78% controlled by Se gene (SeSe or Sese) -codes for the production of fucosyltransferase -utilizes Type 1 precursors |
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non-secretors
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-homozygous for se se
-do not secrete ABH substances |
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A1 cells
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-80%
-Reacts w/ anti-A and A1 -attaches Galnac to branched chains -Low/High level of H/A |
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A2 cells
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-20%
-Reacts w/ only anti-A -attaches Galnac to stright chains -High/Low level H/A |
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Anti-A1
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1-8% of A2 have anti-A1
22-35% of A2B have anti-A1 -Dilichous Biflorous= source |
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Anti-H
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-produced by bombay
-ulex europaeus |
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Wharton's Jelly
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-Viscous mucopolysaccharide
-Cord blood -Spontaneous rouleaux -Wash cord cells 6-8 times with saline |
