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26 Cards in this Set
- Front
- Back
- 3rd side (hint)
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CMV
Laboratory diagnosis
How many? |
3
List |
Cell culture Detection of CMV Ab Detection of CMV Ag |
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CMV
Laboratory diagnosis
3 List |
Cell culture Detection of CMV Ab Detection of CMV Ag |
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CMV
Laboratory diagnosis
_________ Detection of CMV Ab Detection of CMV Ag |
Cell culture
Description |
Definitive method |
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CMV
Cell culture
Description |
Definitive method
Notes: obs and time |
Look for cytopathic effects
Takes several days to weeks to get results |
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CMV
Cell culture ______________ Detection of CMV Ag |
Detection of CMV Ab
Most common methods? |
ELISA Latex agglutination |
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Detection of CMV Ab
Most common methods? How many? |
2
List |
ELISA Latex agglutination |
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Detection of CMV Ab
2 common methods |
ELISA Latex agglutination |
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Detection of CMV Ab
What Antibodies are detected? |
IgM Total Ab
How they are used? |
IgM: Infants IgM or Total Ab: Adults |
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Detection of CMV Ags
Method |
Direct immunofluorescence
How many steps? |
3 |
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Detection of CMV Ags Method: Direct immunoflourescence
How many steps? |
3
List |
Clinical specimen is inoculated into cell line
Incubate and fix onto slide
Add Ab to CMV Ag-fluor. dye
Incubate and wash
Observation with fluorescent microscope |
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Detection of CMV Ags Method: direct immunoflourescence
3 steps |
Clinical specimen inoculate into cell line
Incubate and fix on slide
Add Ab to CMV Ag-Flour. dye
Incubate and wash
Observe with fluor. microscope |
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Detection of CMV Ags Method: direct immunoflourescence
Interpretation |
+=Flour. in nuclei of infected cells
Results are obtained ______ after inoculation |
16-24 hours |
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EBV
Types of test |
Screening Confirmatory |
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EBV
Two types of test |
Screening COnfirmatory |
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EBV
___________ Confirmatory |
Screening test
Description |
Heterophile Ab |
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EBV
Screening test
Description |
Heterophile Ab
Notes |
Screening for infectious mono is performed by looking at the heterophile Ab, which reacts with horse, bovine and sheep rbcs |
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EBV
Screening test ____________ |
Confirmatory test
Description |
Detection of Ab for EBV-specific Ag by ELISA or IFA |
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EBV
Confirmatory test
Description |
Detection of Ab for EBV-specific Ag by ELISA or IFA
Notes |
Ab for EBV-specific Ag can be detected by ELISA or IFA in heterophile Ab-negative cases of infectious mono or in other EBV-associated disorders. |
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Detection of Rubella, Rubeola or Varicella Abs in Pt. serum
How many types? |
3 types
List |
Test for Immunity Test for acute infections Test for congenital infection |
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Detection of rubella, rubeola, or Varicella Abs in Pt. serum
3 types
_________________ Test for acute infections Tests for congenital infection |
Test for immunity
Description |
Virus specific IgG |
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Detection of rubella, rubeola, or Varicella Abs in Pt. serum
3 types
Test for immunity ________________ Test for Congenital infection |
Test for acute infections
Description |
Virus-specific IgM 4-fold rise in virus specific IgG Abs |
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Detection of rubella, rubeola, or Varicella Abs in Pt. serum
3 types
Test for immunity Test for acute infection _____________ |
Test for congenital infection
Description |
IgM |
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Detection of Rubella, Rubeola, or Varicella Abs in Pt. serum
It can be detected by? |
ELISA Latex agglutination |
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Hepatitis A
Serologic markers |
IgM-anti-HAV
Clinical significance |
Current infection |
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Hepatitis B
Serological markers |
HBsAg Hepatitis B surface antigen
Anti-HBs |
HBsAg +: Pt is infectious
Anti-HBs +: Pt has immuntiy |
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Hepatitis C
Serologic markers |
Anti-HCV
Clinical significance |
Exposure to Hepatitis C |
