Hematology Lab Techniques

Front 1

The battlement scan pattern is employed in an eye-count differential examination of a wedge blood film b/c...

Back 1

Eosonophils occur more frequently in the center of the film

Front 2

The battlment scan pattern is employed in an eye-count differential exam of a wedge blood film b/c

Back 2

Eosonophils occur more frequently in the center of the film.

Hint 2

cell that occurs in the center.

Front 3

RBCs that vary in size from the normal 6-8 microns are described as exibiting:

Back 3

anisocytosis

Hint 3

not poikilocytosis

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mean cell volume (MCV) is calculated using the formula

Back 4

HCT X 10
________
rbc count

Hint 4

deals with hct and rbc

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ON A STAINED PERIPHERAL BLOOD SMEAR, WHAT TERM DESCRIBES THE CHANGE IN SHAPE OF RBCS:

Back 5

POIKILOCYTOSIS

Hint 5

NOT ANISOCYTOSIS

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SMALL LYMPHOCYTES WILL BE CONCENTRATED ON WHAT PART OF THE WEDGE SMEAR

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CENTER OF THE FILM

Hint 6

SAME AS EOSONOPHILS

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AN RBC CONTAINING IRON GRANULES IS STAINED WITH PRUSSIAN BLUE, THE CELL IS A

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SIDEROCYTE

Hint 7

NOT A SCHISTOCYTE BUT A

Front 8

A 7 ML EDTA TUBE IS RECEIVED IN THE LAB CONTAINING ONLY 2 ML OF BLOOD. IF USING MANUAL TECHNIQUES, WHAT RESULT WILL BE ERRONEOUS

Back 8

HCT

Hint 8

EXCESSIVE ANTICOAGULANT CAUSES RBC SHRINKAGE RESULTING IN

Front 9

MCHC IS THE AVERAGE...

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CONCENTRATION OF HGB IN RED CELLS EXPRESSED AS A PERCENTAGE.

Front 10

MCHC IS THE RATIO OF THE ...

Back 10

WEIGHT OF HGB TO THE VOLUME OF RBC.

Front 11

MCHC CALCULATION

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HGB
_____ x 100
hct

Front 12

IF MCHC IS LOW THIS INDICATES THE RBCS ARE...

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HYPOCHROMIC

Hint 12

HAS MORE CENTRAL PALLOR.

Front 13

1;200 DILUTION OF A PT SAMPLE WAS MADE. 336 RBC WERE COUNTED IN AN AREA OF .2MM2 WHAT IS THE RBC COUNT?

Back 13

RBC COUNT =
336 x 200 x 10 = 672,000
------- = 3.36 X 10 12TH/L
.2

Hint 13

RBC COUNT =
RBC X DILUTION X DEPTH,
_______________________

AREA

Front 14

RBC COUNT FORMULA

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RBC X DILUTION X DEPTH
______________________
AREA

Hint 14

THINK OF HEMACYTOMETER

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WHAT PHAGOCYTIC CELL PRODUCES LYSOZYMES THAT ARE BACTERIALCIDAL...

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NEUTROPHILS

Hint 15

CAN BE SEGMENTED OR BANDS

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CORRECTED RETICULOCYTE COUNT FORMULA...

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% RETIC X HCT
_____________
45 (AVG NML HCT)

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A DECREASED RESULT IN AN OSMOTIC FRAGILITY TEST WOULD BE ASSOC WITH THESE TYPE OF CELLS AND CONDTION CONDITIONS

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SICKLE CELL ANEMIA
TARGET CELLS
POIKILOCYTES ARE PRESENT

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WHAT EFFECT WOULD USING BUFFER AT PH 6.0 HAVE ON A WRIGHT STAINED SMEAR?...

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WHEN THE PH IS TOO LOW <6.4, THE RBC TAKE UP MORE ACID DYE (EOSIN), BECOMING TOO PINK. lEUKOCYTES HAVE POOR NUCLEAR DETAIL.

Hint 18

RBC HAVE TO COMPENSATE, TOO LITTLE BUFFER, GET SOME MORE...

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WHAT RBC INCLUSION CAN BE VISUALIZED WITH SUPRAVITAL STAIN, BUT CANNOT BE DETECTED ON A WRIGHT STAIN SMEAR?

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HEINZ BODIES

Hint 19

IRREGULAR, REFRACTILE, PURPLE INCLUSIONS THAT ARE NOT VISIBLE WITH WRIGHT STAIN.

Front 20

A FALSELY ELEVATED HCT IS OBTAINED FROM USING A DEFECTIVE CENTRIFUGE. WHAT WILL BE AFFECTED.

Back 20

MCV, MCHC, RDW

Hint 20

NOT MCH

Front 21

THIS REFLECTS THE VARIANCE IN SIZE OF THE RBCS..

Back 21

RDW

Front 22

FORMULA FOR MCV

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HCT X 10
--------
RBC

Front 23

FORMULA FOR MCHC

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HGB X 100
---------
HCT

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FORMULA FOR MCH

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HGB X 10
--------
RBC

Hint 24

NOT AFFECTED BY HCT

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MILLER DISK IS AN OCULAR DEVICE USED TO FACILITATE COUNTING OF

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RETICULOCYTES

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WHAT STAIN METHOD IS USED MOST FREQUENTLY TO STAIN AND COUNT RETICS?

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SUPRAVITAL STAINING WITH METHYLENE BLUE

Hint 26

NOT WRIGHT STAIN

Front 27

THE COULTER PRINCIPLE FOR CELL COUNTING IS BASED UPON

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ISOTONIC SOLUTIONS CONDUCT ELECTRICITY BETTER THAN CELLS

Front 28

A CORRECTION IS NECESSARY FOR WBC COUNTS WHEN NRBCS ARE SEEN IN THE PERIPHERAL SMEAR B/C

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NRBCS ARE COUNTED AS LEUKOCYTES.

Front 29

CORRECTED WBC COUNT

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WBC COUNT X 100
---------------
100 + NRBC

Front 30

COULTER RDW CORRELATES WITH THE DEGREE OF

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ANISOCYTOSIS

Hint 30

SIZE VARIATION

Front 31

REFERENCE RANGE FOR RDW

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11.5%-14.5%

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OSMOTIC FRAGILITY TEST RESULT IN A PT WITH THALASSEMIA MAJOR WOULD MOST LIKELY BY

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DECREASED BC TARGET CELLS ARE SEEN AND THEY HAVE INCREASED SURFACE VOL.

Hint 32

TARGET CELLS

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HYPERSPLENISM IS CHARACTERIZED BY

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LEUKOPENIA

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WHICH MORPHOLOGICAL CLASSIFICATION IS CHARACTERISTIC OF MEGALOBLASTIC ANEMIA

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MACROCYTIC, NORMOCHROMIC

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PERNICIOUS ANEMIA IS A LACK OF ?

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INTRINSIC FACTOR THAT PREVENTS VIT B12 ABSORPTION

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CHARACTERISTICS OF MEGALOBLASTIC ANEMIA

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PANCYTOPENIA
HYPERSEGMENTED NEUTROPHILS
MACROCYTIC RBC
DECREASE RETIC COUNT

Front 37

A PT WITH B12 DEFICIENCY ANEMIA IS GIVEN A HI DOSE OF FOLATE. WHAT IS THE EXPECTED RESULT

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AN IMPROVEMENT IN THE HEMATOLOGICAL ABNORMALITIES.

Hint 37

IMPROVEMENT IN....

Front 38

WHICH ORGAN REMOVES RBC INCLUSIONS WITHOUT DESTROYING THE CELL

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SPLEEN

Hint 38

SUPREME FILTER

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MORPHOLOGICAL CHARACTERISTICS OF IRON DEFICIENCY ANEMIA INCLUDE

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MICROCYTIC,HYPOCHROMIC
PLATELETS SMALL AND INCREASED
INCREASE IN TIBC
INCREASE IN RBC PROTOPORPHYRIN

Front 40

FE DEFICIENCY ANEMIA MAY BE DISTINGUISHED FROM ANEMIA OF CHRONIC INFECTIONS BY

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TIBC

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IN FE DEFICIENCY ANEMIA THE SERUM IRON LEVElS IS ___ AND THE TIBC IS ___

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DECREASED, INCREASED

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IN CHRONIC FE DEFICIENCY ANEMIA THE IRON IS

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TRAPPED IN THE RE CELLS AND ARE UNAVAILABLE TO RBCS. SERUM FE AND TIBC ARE DECREASED.

Front 43

IRON DEFICIENCY ANEMIA RBC MORPHOLOGY

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MICROCYTIC,HYPOCHROMIC

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FE DEFICIENT ANEMIA IS CAUSED BY DEFECTIVE

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HEME SYNTHESIS

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THALASSEMIA IS CAUSED BY

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DECREASED GLOBULIN CHAIN SYNTHESIS.

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STORAGE OF IRON IS USUALLY BEST DETERMINED BY

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SERUM FERRITIN LEVELS