Hematology Ii Final Study Slides

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Punch-out vac blasts (L3 burkitt's conversion)

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HgB S (sickle cell trait/disease)

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Rouleaux (MM, plasma cell leukemias)

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Plasma cell (Ig's are blue)

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Motte cell (russel bodies Ig's)

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Flame Cell (IgA, MM, plasma cell leukemia)

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Auer rods

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M3 (Promyelo)

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M3 (Auer rods, Promyelo)

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M3m (associated with DIC granules activate PLT)

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Schistocytes (Hemolytic anemia, DIC)

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Pappenheimer (Unused iron)

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Ringed sideroblast (prussian blue) may see in MDS

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Basophillic stippling (coarse=lead poisoning/sideroblastic anemia)

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Megaloblastic anemia (look for B12/anti-IF/anti-PC/folate)

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Left shift (CML vs leukomoid)

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LAP stain (increased in leukomoid, lowered in CML)

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Hairy cell leukemia (TRAP POS)

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TRAP pos (Hairy cell) acid phosphatase isoenzyme #5

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Hypochromic microcytic (Thal, Hgbopathies, sideroblastic anemia, AOI, IDA)

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Elliptocytosis (spectrin-ankyrin defect horizontal)

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M5a (MPO+ SBB+ Spe= Nsp +++ NaF neg)

No mature component

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Malaria

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Malaria (lyses cells to cause problems)

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Many blasts and NRBC in M6a

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Granulocytes and nucleated RBC in M6a

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Nucleated RBC in M6a

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Pas +, coupled with myelocyte component makes M6a

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HgB SC

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Promyelocytes/Blasts

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Heinz bodies on Supravital stain (denatured Hgb). Inidcate G6PD def. Alpha thal (H and barts)

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Smudge cells (ALL/CLL) clear smudging with ALBUMIN

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Pelger hewit cells

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Blasts (LYMPH)

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PAS + (ALL)

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Sphereocytes and Retics=hemolysis likely

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Spherocytes. Confirm with osmotic fragility (increased)

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Beta thal hemolysis, Alpha-only HgB are unstable and destroy cells.

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ET or M7 (too many PLT)

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ET or M7, too many megakaryocytes

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Kleihauer betke stain=look for FMH. HgB F doesn’t elute in acid.

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Blasts

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MPO +

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SBB +

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PAS =

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Spe +

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Nsp w+ likely M1

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M2, increased BASO in blood likely

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M4 (MPO + SBB+ SE +, NSE +++ NaF w+)

SE and NSE indicate myeloid component

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Reed Sternberg cells, Hodgkins lymphoma (see only in lymph)