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31 Cards in this Set

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What are the 2 common phenotypes of Staphylococci?
1.coagulase +
2. thermostable nuclease +
(Staphylococcus Aureues)
But not reliable, look for ENTEROTOXIN
Ingestion of Staphylococcal enterotoxin are intoxication or infection?
intoxication >20ng
How many enterotoxins are identified?
13
A.B.C1.C.2.C3.D.E.G.H.I.J.K.L.
NO F
Mode of action of Staphylococcal enterotoxin
1. act as superantigen that mediates cross linkage between T cells receptor (CD4 T cells) and class II MHC (APC)
2.cross linkage stimulates massive inflammatory responses and releases cytokines such as IL2
Staphylococcal enterotoxin are made out of heat resistant protein.....
Which heat resistrance come first
SEC>SEB>SEA
CBA
Treament: Drink a lot of water to maitin fluid balance,rest
Which bacteria it refer to?
Staphylococcal enterotoxin
Gram + , spore forming and aerobic rods is which Bacteria
Bacillus cereus
What are the 2 type of illness for Bacillus cereus gastroenteritis?
Diarrheal
and emetic
Emetic syndrome is similar to which type of bacteria ?
Staphylococcal food poisoning
Diarrhea syndrome is similar to which type of bacteria
C. perfringens food poisoning
what is the key virulence factor for Bacillus cereus enterotoxins?
Hemolysin BL (HBL)
Hbl shows hemolysis, cytolysis, dermonecrosis, vascular permeability enterotoxic activity
What kind of toxin is called cereulide,
short peptide with ring structure,
heat stable, cause vomiting, RICE
EMETIC TOXIN
Bacillus cereus
What type of bacteria are gram positive ,spore forming and anaerobic rods
Clostridium Perfringer
What type of bacteria is highly fermentative and produce gases
Clostridium Perfringer
Which bacteria are classified into 5 groups based on toxins produced
Clostridium Perfringer
Describe Clostridium Perfringens enterotoxin (CPE) with 4 details
1.key virulence factor of type A
2.Synthesized during sporulation
3.Heat sensitive
4.Resistant to trypsin and chymotrypsin
Mode of action of Clostridium Perfringer enterotoxin (CPE)
Bind to host receptor---> form large complex--->change membrane permeability---->death of host cells.
Which bacteria cell are sensitive to pH and are easily killed by the acidity in stomach
Clostridium Perfringer
Which bacteria is among the 3rd 4th foodborne disease in US
Clostridium Perfringer
All c.botulinum stairs produce........ which cause botulism
botulinum neurotoxin
C.botulinum
Bacteria are classified based on their .......... and ............?
1.Toxin type
2. Physiological characteristic
C.botulinum
C.botulinum neurotoxin is the most toxic natural subtance know. It is also an ..... toxin?
A-B toxin
a:activity domain(light chain)
b. binding domain ( heavy chain)
which type of botulism in intoxication?
only 1
Food borne botulism
-consumption of toxin pre - formed in food
Which 3 types to botulism is infection?
1.Infant botulism-growth of c.botu in GI
2.adult botulism-growth and production of toxin in adult intestine
3.wound botulism- grow and produce toxin in would like tetanus
Which bacteria are the most resistant spores?
C.botulinum
which type of bacteris is gram positive and non spore forming rods??
Listeria monocytogenes
which bacteria cause the most number of recalls and hospitalization?
Listeria monocytogenes
Listeria monocytogenes are monitored by which 2 agency
Foodnet and pulse net
which bacteria attack immuno suppressed individuals, newborn and pregnant
Listeria monocytogenes
What are the 3 virulence factors for Listeria monocytogenes
1. internalin - binding and internalization
2.listeriolysin- has beta hemolytic activity and is a cytotoxin
3.ActA triggers host actin polymerization
HowListeria monocytogenes spreads from cell to cell
1.L. mon triggers actin rearrangement in host cell
2. form a tail of actin polymers
3.The actin tails push the bacterial cells through host cells