Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
7 Cards in this Set
- Front
- Back
|
SNP - single nucleotide polymorphisms |
Single base-pair differences between individuals - many genetics disorders are due to a single base-pair change in the DNA of the related disease gene |
|
|
RFLP - restriction fragment length polymorphism |
Variation in the lengths of fragments generated by treatment of DNA with a particular restriction enzyme. They result from point mutations that create or destroy restriction enzymes cleavage sites. |
|
|
PCR-RFLP Analysis |
1) Design primers to PCR-amplify a region containing the point mutation 2) Digest the amplified fragment with the RE (BamHI) 3) Use gel to determine the sizes of fragments produced 4) Mutant DNA will give a different digestion pattern |
|
|
STR - Short tandem repeats |
Aka microsatellites PCR detection; for forensics A type of DNA polymorphism involving variation in the number of short, identical sequences that are tandemly repeated at a particular locus in the genome. |
|
|
VNTR - variable number of tandem repeats |
Aka minisatellites RE digest and southern blotting; paternity testing A type of DNA polymorphism involving variation in the number of identical sequences (7 bp+) that are tandemly repeated at a particular locus in the genome |
|
|
Unique VNTR |
Only one copy present in the genome (each person has 2 bands) |
|
|
Multicopy VNTR |
Many copies scattered around the genome A probe that detects a family of this loci may reveal multiple bands in an autoradiogram |
