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20 Cards in this Set
- Front
- Back
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examples: radiation
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UV radiation
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Lesion name & description: radiation
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pyrmidine dimer formed when e- vacate double bond and make 2 sing covalent bonds
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Mutations: radiation
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Sub/Del
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Direct repair: radiation
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Photolyase breaks 2 sing cov bonds using solar energy b4 replication
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Excision Repair: radiation
name enzymes involved |
NER makes 2 nicks on either side of phosphodiester bond
enzymes: UVRabc endonuclease, helicase, DNAPI, and ligase |
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mutation examples: alkylating agents
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nitrosamines, BaP
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lesion name and descr: alkylating agents
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nitrosamines (small ring): AP site causes problem binding sequence
nitrosamines (large ring): methyl guanine BaP: alkyl guanine |
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mutation: alkylating agents
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AP site = sub/del
meth guanine = transition alkyl guanine = transversion |
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direct repair: alkylating agents
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suicide enzye removes ethyl/methyl then is degraded in proteosome
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Excision repair: alkylating agents
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AP endonuclease fix AP sites
NER fixes alkyl guanine |
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examples: deaminating agents
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nitrous acid
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lesion name & descr: deaminating agents
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normal base is turned into rare base which occurs when exocyclic amine turns into a carbonyl
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Mutation: deaminating agents
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Transitions
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Direct repair: deaminating agents
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none
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Excision repair: deaminating agents
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Glycosylase detects rare base and removes it. AP endonuclease is repair system
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examples: intercalators
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ethidium bromide
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lesion name & descr: intercalators
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slide b/w bases and backbone stretches which causes new strand to not stay together which increases frequency of slippage
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mutation: intercalators
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ins/del
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direct repair: intercalators
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none
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excision repair: intercalators
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mismatch repair fixes slippage. mutS binds to mismatch and brings mismatch to mutH that is bound to methyl, mutH binds to mutS activating mutH's endonuclease act, mutH nicks @ methylation site, then exonucl removes until mismatch, mutS indicates where it should stop
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